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Fast stem cell homing method

A stem cell and fast technology, applied in magnetic therapy, electrotherapy, treatment and other directions, can solve the problem of stem cell reduction, retention, affecting repair function, etc., and achieve the effect of efficient and fast homing and good clinical application prospects.

Inactive Publication Date: 2013-04-24
北京清美联创干细胞科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although the former can ensure that the stem cells can be relatively concentrated in specific tissues, it needs to be realized through open surgery, which is complicated, causes great trauma to the recipient, and many complications; the latter method is simple and easy to operate, but because the selected stem cells circulate with the blood It flows to various organs of the whole body, so it is easy to be swallowed and retained by certain tissues rich in reticuloendothelial system, which makes the number of stem cells located in specific tissues relatively reduced, which seriously affects their repair function

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1, c-Met + Homing of β2M cells

[0016] In this example, the liver of a rat model of liver injury was used as the target tissue. The immunomagnetic beads used in this example were purchased from the School of Materials, Tsinghua University. The magnetic beads were coupled with goat anti-rabbit IgG, with a particle size of about 35 nm and good superparamagnetism.

[0017] 1. Obtaining rat bone marrow mononuclear cells

[0018] Rats sacrificed by neck dissection were sterilized with 75% ethanol, and the femur was taken out by laparotomy. The DMEM culture solution containing 10% fetal bovine serum was used as the flushing solution, and the bone marrow cavity was washed repeatedly with a 5ml syringe, and filtered through a 100-mesh sieve. The obtained cells were made into rat bone marrow single cell suspension, and after centrifugation at 1200rpm, the cells were resuspended in PBS, and rat bone marrow mononuclear cells were collected after density gradient centrif...

Embodiment 2

[0028] Embodiment 2, the detection of cell homing effect

[0029] In vitro enrichment

[0030] The β2M cells labeled with immunomagnetic beads were obtained according to steps 1-2 in Example 1, and washed with PBS. Physiological saline containing 20% ​​dextran was then prepared as a mobile phase, and the resulting cells were suspended therein. Adjust the flow rate of the peristaltic pump to 0.1-15cm / s, so that the cells labeled with magnetic beads flow through the magnetic field (adjust the distance between the two magnetic poles to 1.6-2.5cm, so that the field strength fluctuates at 1.33-0.85T), count the outflow and inflow cells under a microscope The ratio of the magnetic field to the enrichment efficiency of the magnetic bead-labeled cells was obtained. The operation process is as Figure 4 As shown, 15 in the figure is the 20% dextran saline solution of β2M cells labeled with immunomagnetic beads; 16 is the peristaltic pump; 17 is the two magnetic poles of the magnetic...

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Abstract

The invention provides a method which enables stem cells to be homed quickly and effectively. The stem cell homing method includes the steps that immunomagnetic beads are used for marking the stem cells, and the particle size of each immunomagnetic bead is 40-60 nanometers; the marked stem cells are injected into a circulatory system of specific tissue; and a positive magnetic pole and a negative magnetic pole point to body surface portions corresponding to the specific tissue, and in the circulation process of the circulatory system, the stem cells are homed into the specific tissue quickly and accurately, wherein the magnetic field strength between the positive magnetic pole and the negative magnetic pole is 8,400-18,600 gausses. According to the stem cell homing method, the magnetic characteristic of the superparamagnetism magnetic beads is utilized innovatively, the magnetic attraction technology is adopted to enable the stem cells to be homed into the specific tissue, the stem cells can be helped to differentiate into functional adult cells in the specific tissue, and a new direction is exploited for clinical application of the stem cells.

Description

technical field [0001] The present invention relates to a shortcut method for stem cell homing, specifically using a magnetic field to make stem cells homing rapidly and accurately. Background technique [0002] Stem cells refer to a class of cells with self-renewal ability and multi-directional differentiation potential, which can be differentiated into different functional cells under appropriate induction conditions. With the deepening of stem cell biology research, cell therapy using stem cells as seed cells has been widely used in diabetes, liver failure, myocardial infarction, Alzheimer's disease, Parkinson's disease, retinal damage, cancer, hemophilia, spinal cord injury, innate immunity It has increasingly shown good application prospects in the treatment of refractory and end-stage diseases such as chronic diseases. Studies have found that adult stem cells derived from various tissues (including bone marrow, umbilical cord blood, etc.) in mammals have the character...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61N2/00
Inventor 田杰
Owner 北京清美联创干细胞科技有限公司
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