The invention relates to the technical field of
biology, in particular to a recombinant vector and a stable
cell strain containing the recombinant vector. The recombinant vector and the stable
cell strain containing the recombinant vector can be used for screening
stem cell homing promotion drugs. The recombinant vector contains a CXCR4
promoter sequence and a luc2
luciferase report
gene; and theluc2
luciferase report
gene is positioned at the downstream of the CXCR4
promoter sequence. The stable
cell strain which contains the recombinant vector and an internal reference
luciferase vector canbe used for detecting the expression
activity level of the CXCR4
gene, and then are used for screening the
stem cell homing promotion drugs at high
throughput. Therefore, the stable
cell strain is added in a candidate
drug for stimulation, through luc2 relative
fluorescence intensity, the activity of promotion of the candidate
drug on expression of the CXCR4
promoter can be evaluated, therefore,the
stem cell homing drugs or drugs for treating
myocardial infarction or assisting in treatment of the
myocardial infarction are screened, and the application prospect is wide.