Method for improving yeast beta-D-glucan water-solubility

A dextran and water-soluble technology, which is applied in the field of improving the water solubility of yeast β-glucan by using ionic liquids, can solve the problems of high production cost, damage, and low yield of soluble glucan, and achieve industrial scale production , The effect of simple process operation

Active Publication Date: 2013-04-24
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the methods for modifying solubilized yeast β-D-glucan mainly include sulfation, carboxymethylation, and β-glucanase. D-glucan needs to use chemical reagents such as concentrated sulfuric acid, dimethyl sulfoxide, and chloroacetic acid, which not only cause pollution and damage to the environment, but also cannot achieve industrial scale production; use enzymatic modification to solubilize yeast β-D- Dextran, soluble dextran has a low yield and high production cost, and is not suitable for industrial production

Method used

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  • Method for improving yeast beta-D-glucan water-solubility
  • Method for improving yeast beta-D-glucan water-solubility
  • Method for improving yeast beta-D-glucan water-solubility

Examples

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Embodiment 1

[0033] Example 1. Modified solubilized yeast β-D-glucan

[0034] (1) Add yeast β-D-glucan to 1-ethyl-3-methylimidazole acetate ionic liquid, and heat to 85°C until yeast β-D-glucan is completely dissolved in the ionic liquid , To obtain a glucan-ionic liquid mixed solution, in which the mass percentage of yeast β-glucan is 0.5%;

[0035] (2) The dextran-ionic liquid mixed solution obtained in step (1) is subjected to micro-jet homogenization treatment with a high-pressure micro-jet homogenizer, and the pressure of the micro-jet homogenization treatment is controlled to 170 MPa and the treatment flow rate is 22 mL / min;

[0036] (3) Precipitate the dextran in the dextran-ionic liquid mixed solution after the high-pressure micro-jet homogenization treatment in step (2) with ethanol, and the volumetric amount of ethanol is that of the dextran-ionic liquid mixed solution Precipitate at 4°C for 24h, then centrifuge and evaporate to remove the ethanol in the supernatant, and then heat and ...

Embodiment 2

[0044] Example 2: Modified solubilized yeast β-glucan

[0045] (1) Add yeast β-D-glucan to 1-ethyl-3-methylimidazole acetate ionic liquid, and heat to 85°C until yeast β-D-glucan is completely dissolved in the ionic liquid , To obtain a glucan-ionic liquid mixed solution, in which the mass percentage of yeast β-glucan is 1.0%;

[0046] (2) The dextran-ionic liquid mixed solution obtained in step (1) is subjected to micro-jet homogenization treatment with a high-pressure micro-jet homogenizer, and the pressure of the micro-jet homogenization treatment is controlled to be 140 MPa and the treatment flow rate is 30 mL / min;

[0047] (3) Precipitate the dextran in the dextran-ionic liquid mixed solution after the homogenization of the high-pressure micro-jet in step (2) with ethanol, and the volumetric amount of ethanol is that of the dextran-ionic liquid mixed solution 3 times, precipitate at 25°C for 5h, then centrifuge, rotary evaporate to remove the ethanol in the supernatant, and the...

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Abstract

The invention discloses a method for improving yeast beta-D-glucan water-solubility. The method comprises the steps that: (1) yeast beta-glucan is dissolved by using an ionic liquid, such that a glucan-ionic liquid mixed solution is obtained; (2) the glucan-ionic liquid mixed solution is subjected to a micro-jet homogenization process; (3) an organic solvent is added into the homogenized glucan-ionic liquid mixed solution, such that precipitation is carried out; (4) the precipitate is dissolved by using water, and centrifugation is carried out, such that a supernatant is obtained, and the improvement upon the yeast beta-glucan water-solubility is realized. With the method provided by the invention, modified and solubilized yeast beta-D-glucan is white fluffy cottony solid. With the method provided by the invention, yeast beta-glucan application range can be widened.

Description

Technical field [0001] The invention relates to a method for modifying and solubilizing yeast β-D-glucan, in particular to a method for improving the water solubility of yeast β-glucan by using ionic liquid. Background technique [0002] Yeast β-D-glucan accounts for 30-60% of the dry weight of yeast cell walls, and the diameter is generally 20-80μm. The main chain is connected by β-1,3 glycosidic bonds, and β-1,6 glycosidic bonds are interspersed between the chains. , Is a linear molecule polymerized by 1500 glucose residues. Its molecular weight is about 240KDa. It can enhance immunity, anti-inflammatory, antibacterial, anti-infection, anti-virus, anti-cancer, lowering cholesterol, anti-radiation, healing wounds, etc. Many physiological functions. Due to the interaction of the polyhydroxyl groups in the yeast β-D-glucan molecule to form a dense triple helix structure, it is insoluble in water. Water insolubility not only restricts its application in food, medicine, cosmetics a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C08J3/00C08J3/09C08L5/02
Inventor 王强刘红芝刘丽高洁胡晖
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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