Mosquito-killing gel and preparation method thereof
A mosquito gel, gel technology, applied in the field of mosquito gel, can solve the problems of difficult to meet the practical application, poor vitality, etc., to achieve good application prospects, long duration, and good stability
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Embodiment 1
[0031] The preparation of embodiment 1 mosquito-killing gel of the present invention
[0032] Take 1 / 4KPYG 2 Medium (composition: peptone 0.375g, yeast extract 0.125g, glucose 0.75g, cholesterol 6.25mg, calcium chloride 27.5mg, corn oil 2.5g, add water to 1L), add trehalose 10g, corn oil 25g, OP-100.5 g, adjust the pH value to 7.0~10.0, add 1g of agar powder, dissolve, cool to 60°C, add Pythium sp.GY1938 spores until the number is 5×10 10 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.
Embodiment 2
[0033] Embodiment 2 The preparation of mosquito-killing gel of the present invention
[0034] Take 2×KPYG 2 Medium (composition: peptone 3g, yeast extract 1g, glucose 6g, cholesterol 50mg, calcium chloride 220mg, corn oil 20g, add water to 1L), adjust the pH value to 7.0~10.0, add agar powder 8g, dissolve, cool to 40 ℃, add Pythium sp.GY1938 spores to a quantity of 1×10 11 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.
Embodiment 3
[0035] Embodiment 3 Preparation of mosquito-killing gel of the present invention
[0036] Take 1 / 2KPYG 2 Culture medium (composition: 0.75g peptone, 0.25g yeast extract, 1.5g glucose, 12.5mg cholesterol, 55mg calcium chloride, 5g corn oil, add water to 1L), add 25g trehalose, 30g corn oil, OP-101g, adjust When the pH value reaches 7.0~10.0, add 2g of agar powder, dissolve, cool to 60°C, add Pythium sp.GY1938 spores until the number is 1×10 10 Homogenize and stir to obtain a gel suspension, put it in a mold immediately, cut into pieces to shape after solidification, and package and store at 4-8°C.
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