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Separation and purification method of colistin sulfate

A technology of colistin sulfate and purification method, which is applied in the field of separation and purification of colistin sulfate, can solve the problems of large amount of sulfuric acid, complicated process, long cycle, etc., and achieves the advantages of improving yield, less degradation and avoiding titer reduction. Effect

Active Publication Date: 2014-10-15
SANDA FILM SCI & TECH XIAMEN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above route of the prior art has the disadvantages of large sulfuric acid consumption, complicated process and long cycle time

Method used

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  • Separation and purification method of colistin sulfate
  • Separation and purification method of colistin sulfate
  • Separation and purification method of colistin sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The titer of the fermentation broth is 550,000 units, and the pH is adjusted to 4.0 with 40% sulfuric acid.

[0022] Ceramic membrane filtration: The ceramic membrane is selected as model 19-3.8, the pore size is 200nm, the operating conditions are inlet pressure 3.5bar, outlet pressure 2.0bar, the fermented liquid with pH 4.0 is concentrated twice, the filter residue is treated separately, and the titer of the dialyzed liquid is About 200,000, adjust the pH to 4.5-5, and another batch to 7.5.

Embodiment 2

[0023] Embodiment 2 Continuous ion exchange parameters are determined

[0024] 1. Equipment: The equipment used is a 30-valve continuous ion exchange system provided by Sanda Membrane Technology Co., Ltd., with a single column volume of 900ml, a resin filling volume of 650ml for a single column, and a total volume of 19.5L.

[0025] 2. Feed liquid: The ceramic membrane filtrate in production, adjust pH value 4.5-5.0 and pH value 7.5 or so respectively

[0026] 3. Use of chemicals: H 2 SO 4 Solution (0.5mol / L)

[0027] NaOH solution (1mol / L)

[0028] 4. Water: industrial pure water

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Abstract

The invention discloses a separation and purification method for colistin sulfate. The method adopts the technical scheme that fermentation broth used in production enters a continuous moving bed system after pretreatment; a continuous moving bed comprises 30 resin columns and is divided into three functional zones, the first zone is an adsorption zone comprising columns of No. 1-14, the second zone is a regeneration zone comprising columns of No. 15-24, the third zone is an analysis zone comprising columns of No. 25-30, a mode of multiple columns connected in series is adopted, the feed liquid enters from columns of No. 6-8 and is subjected to primary adsorption, then the feed liquid is subjected to secondary adsorption in columns of No. 9-11, and the feed liquid which has finished the two processes of being absorbed, is mixed with primary feed washing water to enter columns of No. 12-14 to be subjected to final adsorption; and a column No. 30 is an ER column and used for ejecting out pure water in the column by the use of a product so as to prevent water in the column from entering the product. In the process, the flow direction of the liquid is along the resin column number increment direction, and the rotation of the resin columns is along the resin column number decrement direction, so that reverse effect and continuous cycle are realized to achieve continuous production. The yield of the method is 4-5 points higher than that in the prior art.

Description

technical field [0001] The invention relates to the field of chemical industry, in particular to a method for separating and purifying colistin sulfate. Background technique [0002] Colistin Sulphate (Colistin Sulphate, also known as Colistin Sulfate, Anti-Adrene Sulfate, Colistin, Polymyxin E, Anti-Adrene, etc.) molecular formula is C 52 h 98 N 16 o 13 2H 2 SO 4 , the molecular weight is 1352. The pure product is white or off-white powder, easily soluble in water. Heat-resistant, difficult to absorb in the digestive tract, rapid excretion, low toxicity, no side effects, not easy to produce drug-resistant strains, it is one of the safest growth-promoting antibiotics for livestock and poultry. Colistin sulfate is a basic polypeptide antibiotic, which is mainly used to prevent and control the infection of sensitive bacteria and promote the growth of livestock and poultry. Colistin sulfate can bind to the free phosphate of cell membrane lipoproteins, which reduces the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/62C07K1/18
Inventor 许云鹏杜明华何阿云叶亚洲林雄水林丽华方富林蓝伟光
Owner SANDA FILM SCI & TECH XIAMEN
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