Method for extracting tetrahydroxystilbene glucoside from Polygonum multiflorum

A technology of stilbene glycosides and Polygonum multiflorum, which is applied in the field of biomedicine, can solve the problems of high cost, low purity, cumbersome operation methods, etc., and achieve the effects of low reagent and equipment requirements, simple preparation process, and environmental friendliness

Active Publication Date: 2013-05-08
西安岳达生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, it is extremely difficult and expensive to synthesize stilbene glycosides artificially, and the performance of the synthesized stilbene glycosides is not stable enough, and the purity is not high
At present, extracting stilbene glycosides from plants is a commonly used method. Most of the extractions from plants use a large amount of organic solvents for extraction. Still not high, and there is a problem of organic residues

Method used

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  • Method for extracting tetrahydroxystilbene glucoside from Polygonum multiflorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for extracting stilbene glycosides from Radix Polygoni Multiflori, comprises the following steps:

[0024] 1) Dry and grind Polygonum multiflorum, pass through a 20-mesh sieve to obtain Polygonum multiflorum powder, mix 1kg Polygonum multiflorum powder and 10g calcium hydroxide, add 10L water, stir well to obtain Polygonum multiflorum powder suspension;

[0025] 2) Extract the Polygonum multiflorum powder suspension at 60°C under reflux for 3 times, each time for 1 hour, combine the 3 extracts, concentrate to a relative density of 1.1, and obtain a concentrated solution;

[0026] 3) Add 56 L of ethanol with a volume fraction of 90% to the concentrated solution, stir thoroughly for 4 hours, filter to remove the precipitate, and obtain the filtrate;

[0027] 4) Add ethyl acetate of 1 / 3 of the volume of the filtrate to the filtrate each time, extract 3 times, combine the water phases, let stand for 24 hours, and filter the white crystals obtained as stilbene gly...

Embodiment 2

[0029] The method for extracting stilbene glycosides from Radix Polygoni Multiflori, comprises the following steps:

[0030] 1) Dried Polygonum multiflorum, crushed it, passed through a 20-mesh sieve to obtain Polygonum multiflorum powder, mixed 100kg Polygonum multiflorum powder and 1kg calcium hydroxide, added 800L water, and stirred evenly to obtain Polygonum multiflorum powder suspension;

[0031] 2) Extract the Polygonum multiflorum powder suspension at 55°C under reflux for 3 times, each time for 0.5h, combine the extracts for 3 times, and concentrate to a relative density of 1.2 to obtain a concentrated solution;

[0032] 3) Add 2500L of ethanol with a volume fraction of 95% to the concentrated solution, stir thoroughly for 4 hours, filter to remove the precipitate, and obtain the filtrate;

[0033] 4) Add ethyl acetate of 1 / 3 of the volume of the filtrate to the filtrate each time, extract 3 times, combine the water phases, and let it stand for 24 hours. The white crys...

Embodiment 3

[0035] The method for extracting stilbene glycosides from Radix Polygoni Multiflori, comprises the following steps:

[0036] 1) Dried Polygonum multiflorum, crushed it, passed through a 20-mesh sieve to obtain Polygonum multiflorum powder, mixed 50kg Polygonum multiflorum powder and 500g calcium hydroxide, added 450L water, and stirred evenly to obtain Polygonum multiflorum powder suspension;

[0037] 2) Extract the Polygonum multiflorum powder suspension at 60°C under reflux for 3 times, each time for 1 hour, combine the 3 extracts, concentrate to a relative density of 1.1, and obtain a concentrated solution;

[0038] 3) Add 1200L of ethanol with a volume fraction of 95% to the concentrated solution, stir thoroughly for 4 hours, filter to remove the precipitate, and obtain the filtrate;

[0039] 4) Add ethyl acetate of 1 / 3 of the volume of the filtrate to the filtrate each time, extract 3 times, combine the water phases, and let it stand for 24 hours. The white crystals obtai...

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Abstract

The invention discloses a method for extracting tetrahydroxystilbene glucoside from Polygonum multiflorum, and belongs to the technical field of biological medicines. The extraction method comprises the following steps: 1, taking Polygonum multiflorum powder, mixing the Polygonum multiflorum powder with an alkali having a mass being 0.5-3% of the Polygonum multiflorum powder, adding water having a mass 7-9 times higher than the Polygonum multiflorum powder, and uniformly mixing to obtain a Polygonum multiflorum powder suspension; 2, carrying out reflux extraction of the Polygonum multiflorum powder suspension, and concentrating to obtain a concentrate; 3, adding an organic solvent having a volume fraction of 90-95% and having a mass being 2-4 times higher than the concentrate for precipitation, filtering after precipitation completion, and collecting the obtained filtrate; and 4, adding ethyl acetate to the filtrate for extraction, collecting the water phase, allowing the water phase to stand for crystallization, and collecting crystals after crystallization completion to obtain tetrahydroxystilbene glucoside. The method has the advantages of simple preparation technology, low cost, prepared tetrahydroxystilbene glucoside purity of above 95%, no chemical reagent residual of the product, environmental protection of the extraction process, low reagent and equipment requirements, and suitableness for the large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for extracting stilbene glycosides from Radix Polygoni Multiflori. Background technique [0002] Polygonum multiflorum, also known as Shouwu and Chishouwu, is the dry root tuber of Polygonum multiflorum Thunb. Modern medicine shows that Polygonum multiflorum has multiple functions such as enhancing immune function, anti-oxidation, delaying brain aging, improving DNA repair function, prolonging life, lowering cholesterol, and improving fat metabolism. The main components of Polygonum multiflorum are emodin, chrysophanol, rhein and other glucosides, all of which are stilbene compounds. [0003] Stilbene glucoside is a unique bioactive ingredient in Polygonum multiflorum. It has good water solubility and has the function of anti-oxidation and scavenging free radicals. It is of great research value as an effective anti-aging ingredient. Furthermore, stilben...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
Inventor 张天
Owner 西安岳达生物科技股份有限公司
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