Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody

A VHPSMA-L-VLFITC, bispecific antibody technology, applied in the field of anti-PSMA/FITC bispecific antibody and its preparation, can solve the problems of poor stability of polyploid hybridoma cells, time-consuming and laborious, and achieve large-scale convenience. Production and preparation, strong penetration, small molecular weight effect

Inactive Publication Date: 2013-05-08
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, this method must screen a bispecific BsAb from a large number of similar immunoglobulins, which is time-co

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  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody
  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody
  • Bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of prostatic cancer and preparation method of bispecific antibody

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[0027] The present invention will be described in detail below in conjunction with specific embodiments.

[0028] Bispecific antibody (BsAb) refers to an antibody with two antigen binding properties. The two antigen-binding sites of this type of antibody can simultaneously bind to two different antigens or antigenic determinants. One of the antigen-binding sites can bind to the surface antigen of the target cell, and the other can bind to the effector (such as drugs, effector cells). Etc.) Combine. In fact, a bispecific antibody is a hybrid molecule. The structure between the two heavy chains and the structure between the two light chains is different, and the two Fab fragments are also different.

[0029] PSMA is a type II propria protein that exists in the cell membrane of prostate gland epithelial cells. It has high tissue specificity. As early as 1998, Bostwick et al. found that benign prostate epithelium was found in an immunological study on 187 prostate specimens. To the h...

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Abstract

The invention relates to a bispecific antibody of resisting PSMA/FITC (prostate specific membrane antigen/fluorescein isothiocyanate) for early diagnosis and treatment of a prostatic cancer. A single-chain antibody about the PSMA has been reported more at present, but the bispecific antibody of resisting the PSMA/FITC is researched less. The bispecific antibody related to the invention has a full-length nucleotide sequence shown in SEQ ID No.5. The bispecific antibody is produced by a gene recombination technology, and is convenient to produce and prepare at a large scale, small in molecular weight of an antibody, and strong in penetrating power of tumor tissue; micromolecule antibody can easily achieve the diseased region aiming at the anatomical features that the prostate tissue has thick prostate envelope; and treatment and early diagnosis of the prostatic cancer can be achieved by drugs carried by hapten or a marker by combining with prostatic cancer cells of the diseased region by a pre-targeting technology.

Description

technical field [0001] The invention relates to an antibody, in particular to an anti-PSMA / FITC bispecific antibody for early diagnosis and treatment of prostate cancer and a preparation method thereof. Background technique [0002] Prostate cancer is one of the most common malignant tumors in European and American countries, and it ranks second in the incidence of male tumors in Europe and America. In the United States, the incidence of prostate cancer accounts for the first of all malignant tumors, and the mortality rate is second only to lung cancer. In recent years, the incidence of prostate cancer in China has also shown a gradual upward trend. The early diagnosis of prostate cancer has been paid more and more attention by scholars. Following the discovery of prostatic acid phosphatase (PAP) and prostate-specific antigen (PSA), which were widely used in the diagnosis and treatment of prostate cancer, a prostatic cancer that exists in the LNCaP line The glycoprotein i...

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Application Information

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IPC IPC(8): C07K14/46C12N15/13C12N15/70G01N33/68G01N33/574
Inventor 宦怡魏梦绮袁远郑敏文张劲松杨勇任静黄旭方赵亚超李昕宇
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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