Semicarbazide (SEM) derivatization reagent and application method thereof

Abstract

The invention relates to a semicarbazide derivatization reagent and a kit and application thereof in sandwich ELISA (enzyme-linked immuno sorbent assay) detection of semicarbazide. The reagent is aldehyde benzoic acid with a specificity small-molecular marker; and the aldehyde benzoic acid forms a semicarbazide-aldehyde benzoic acid derivative with a specificity small-molecular marker through amino reaction between aldehyde and semicarbazide. The semicarbazide derivatization reagent provided by the invention can simplify the separation and purification steps of semicarbazide derivative, avoids preparation and application of semicarbazide hapten, and can realize detection of a sandwich ELISA method so as to greatly simplify the detection steps, reduce the detection time and operation error and enhance the detection stability, precision and sensitivity.

Description

technical field [0001] The invention belongs to the field of immunochemical detection, and relates to a semicarbazide derivatization reagent, a semicarbazide derivatization method thereof, and a sandwich ELISA detection method of its derivative. Background technique [0002] Nitrofurazone belongs to nitrofuran drugs and is a broad-spectrum antibacterial drug, which is widely used as therapeutic drug and feed additive to control diseases or epidemics in poultry, livestock and aquaculture. Furacillin is clinically manifested as three obvious effects (carcinogenic, teratogenic, mutagenic), which has attracted great attention from various countries. The European Union banned nitrofurazone as a veterinary drug as early as 1995, and Australia and the United States also successively in 2001 and 2002 Relevant laws were promulgated in 2010, and nitrofurazone was used as a prohibited drug for breeding. [0003] Nitrofur is easily degraded in animals, and can be metabolized into furac...

AI Technical Summary

Problems solved by technology

These methods mainly have the disadvantages of requiring large-scale instruments, which cannot be met by general laboratories, long detection time, complicated extraction and purification process of derivatives, low detection throughput, which cannot meet the needs of on-site detection and large sample detection, and can no longer meet the current detection needs.

[0007] In recent years, some enzyme-linked immunoassay techniques for semicarbazides have also been established, but there are widespread problems such as derivative extraction, low purification efficiency, and negative effects of extraction reagents on antibodies, which have a great impact on the stability and accuracy of detection.

And because semicarbazide is a small molecule, it can only be detected by competitive method at present

This method requires the synthesis of haptens. Due to the differences between different batches of haptens and different batches of products, the detection stability is affected.

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