Semicarbazide (SEM) derivatization reagent and application method thereof

A derivatization reagent, semicarbazide technology, applied in the field of immunochemical detection, can solve the problems of complex extraction and purification process of derivatives, negative effects of antibodies, extraction of derivatives, etc., and achieves good application prospects, economic and social benefits, efficient treatment, High stability effect
CN103105488AActive Publication Date: 2013-05-15北京鑫开元医药科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京鑫开元医药科技有限公司
Publication Date
2013-05-15

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Abstract

The invention relates to a semicarbazide derivatization reagent and a kit and application thereof in sandwich ELISA (enzyme-linked immuno sorbent assay) detection of semicarbazide. The reagent is aldehyde benzoic acid with a specificity small-molecular marker; and the aldehyde benzoic acid forms a semicarbazide-aldehyde benzoic acid derivative with a specificity small-molecular marker through amino reaction between aldehyde and semicarbazide. The semicarbazide derivatization reagent provided by the invention can simplify the separation and purification steps of semicarbazide derivative, avoids preparation and application of semicarbazide hapten, and can realize detection of a sandwich ELISA method so as to greatly simplify the detection steps, reduce the detection time and operation error and enhance the detection stability, precision and sensitivity.
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Description

technical field

[0001] The invention belongs to the field of immunochemical detection, and relates to a semicarbazide derivatization reagent, a semicarbazide derivatization method thereof, and a sandwich ELISA detection method of its derivative. Background technique

[0002] Nitrofurazone belongs to nitrofuran drugs and is a broad-spectrum antibacterial drug, which is widely used as therapeutic drug and feed additive to control diseases or epidemics in poultry, livestock and aquaculture. Furacillin is clinically manifested as three obvious effects (carcinogenic, teratogenic, mutagenic), which has attracted great attention from various countries. The European Union banned nitrofurazone as a veterinary drug as early as 1995, and Australia and the United States also successively in 2001 and 2002 Relevant laws were promulgated in 2010, and nitrofurazone was used as a prohibited drug for breeding.

[0003] Nitrofur is easily degraded in animals, and can be metabolized into furac...

Claims

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