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A method for extracting d-ribose from fermentation broth using ion exchange and membrane separation technology

An ion exchange and fermentation broth technology, which is applied in the field of extracting D-ribose from fermentation broth by using ion exchange and membrane separation technology, can solve the problems of reduced membrane filtration speed, affected filtration effect, complicated operation steps, etc. The effect of reducing the extraction and separation steps, and the process is simple and reasonable

Inactive Publication Date: 2016-06-01
JIANGXI CHENGZHI BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the D-ribose fermentation broth has passed through membrane filtration four times according to the removal accuracy, and the operation steps are cumbersome and time-consuming, which affects the product yield.
And because the fermentation broth contains a large amount of metal ions, as the concentration factor increases during the membrane filtration process, metal salts will continue to be precipitated from the clear liquid, which will adhere to the surface of the membrane core during the membrane filtration process, resulting in subsequent membrane filtration. The filtration speed is reduced, and even the membrane pores are blocked, which greatly affects the filtration effect

Method used

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  • A method for extracting d-ribose from fermentation broth using ion exchange and membrane separation technology
  • A method for extracting d-ribose from fermentation broth using ion exchange and membrane separation technology
  • A method for extracting d-ribose from fermentation broth using ion exchange and membrane separation technology

Examples

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Effect test

Embodiment 1

[0019] Pass 100L of D-ribose fermentation broth through a microfiltration membrane with a membrane pore size of 1000nm. The membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 8 times, 25L of dialysis water is added. The microfiltrate passes through the ultrafiltration membrane with a membrane molecular weight cut-off of 5000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the microfiltrate is concentrated 15 times, 25L of dialysis water is added. The filtrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. The ionized liquid is chromatographed by HZ-803 resin, the effluent is concentrated under reduced pressure, the temperature is controlled at 50-60°C, and the vacuum de...

Embodiment 2

[0022] 100L of D-ribose fermentation broth passed through a microfiltration membrane with a membrane pore size of 500nm, the membrane entry pressure was 0.2MPa, the membrane exit pressure was 0.1MPa, and the operating temperature was 25°C. After the fermentation broth was concentrated 10 times, 25L of dialysis water was added. The filtrate passes through an ultrafiltration membrane with a molecular weight cut-off of 2000, the operating pressure is 1.4 MPa, and the operating temperature is 30-35°C. After the microfiltrate is concentrated 15 times, 25 L of dialysis water is added. The ultrafiltrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. According to the follow-up extraction method in Example 1, after the D-ribose crystals were vacuum-dried, a total of 4.71Kg of D-ribose product was obtained. The purit...

Embodiment 3

[0025] 100L of D-ribose fermentation broth passes through a microfiltration membrane with a membrane pore size of 200nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 10 times, 30L of dialysis water is added. The microfiltrate passes through the ultrafiltration membrane with a membrane molecular weight cut-off of 1000, the operating pressure is 1.4MPa, and the operating temperature is 30-35°C. After the separation fluid is concentrated by 15 times, 30L of dialysis water is added. The filtrate passes through the 732-type cation exchange resin column at a flow rate of 2BV / H, and the effluent from the positive column is also directly connected to the 717-type anion-exchange resin column at a flow rate of 2BV / H. According to the subsequent extraction method in Example 1, after the D-ribose crystals were vacuum-dried, a total of 4.85 Kg of D-ribose product was obtained....

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Abstract

The invention discloses a method for extracting D-ribose from a fermentation broth through ion exchange and membrane separation technologies. The method comprises the following steps of: (1) micro-filtration of a fermentation broth; (2) ultra-filtration of the fermentation broth; (3) ion exchange of ultrafiltrate; and (4) chromatography, condensation and crystallization of an ion exchange liquid. The method disclosed by the invention, by simultaneously applying the ion exchange and membrane separation technologies to pretreatment of the D-ribose fermentation broth, improves pretreatment capacity of the fermentation broth, brings about convenience to operation, overcomes shortcoming that solids in the material liquid are separated out after condensing multiple is increased by the way of independently applying the membrane separation technology to pretreatment of the D-ribose fermentation broth, and remarkably improves yield and quality of the D-ribose product.

Description

technical field [0001] The invention relates to a method for extracting D-ribose from fermentation broth, in particular to a method for extracting D-ribose from fermentation broth by ion exchange and membrane separation technology. Background technique [0002] D-ribose exists in all biological cells as a component of the genetic material ribonucleic acid (RNA) and deoxyribonucleic acid (DNA). Its reducing body, ribitol, is an important component of ribitol teicolic acid in the cell membrane, and plays an important role in physiology. [0003] D-ribose is industrially synthesized vitamin B 2 It is an important raw material and an important intermediate of many nucleic acid drugs. Especially in recent years, the demand for D-ribose as a synthetic raw material for anticancer and antiviral drugs, riboflavin and flavor enhancers has gradually increased in the pharmaceutical and food industries. With the in-depth research on D-ribose, the role of D-ribose in health care has gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/02C07H1/06
Inventor 杨光郑雄敏杨世传曾乐黄恒述任祥万
Owner JIANGXI CHENGZHI BIOENG
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