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Industrial horseradish planting method

A planting method and factory-like technology, applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve problems such as difficulty in producing a large number of high-quality healthy seedlings, low seedling reproduction coefficient, unfavorable wasabi promotion, etc., and achieve improved The effect of factory production capacity, low utilization rate, and labor reduction

Active Publication Date: 2015-03-18
四川华阳蓝公府实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen from the above that the traditional seedling breeding method leads to low seedling reproduction coefficient and slow propagation speed, it is difficult to produce a large number of high-quality healthy seedlings, and it is also easy to be infected with ink disease during planting, which is not conducive to the promotion of wasabi planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] (A1) Accelerating germination: select the wasabi varieties that need to be cultivated, and keep the seeds at 18°C-22°C and the humidity at 90% to promote germination;

[0054] (A2) Material selection: When the wasabi seed germinates 1 to 2 cm in length, cut the stem tip and the length is 0.3 to 0.4 cm;

[0055] (A3) Washing: After washing the buds arranged in step (A2) with detergent solution, wrap them with gauze, rinse them with tap water for 2 hours, and then soak them in a 1% concentration of Xiaojingling solution for 30 minutes, and finally Bacteria moisture-absorbing paper basically absorbs water;

[0056] (A4) Sterilization: Soak the stem tip of the wasabi bud prepared in step (A3) with 70% volume concentration of alcohol for 28-32 seconds, then use 0.2% mercury for disinfection, and finally wash with sterile water for 5 times ;

[0057] (A5) Primary cultivation: Use culture medium to cultivate sterile stem tips into sterile wasabi seedlings;

[0058] (A) Rooting cultiva...

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Abstract

The invention discloses a horseradish seedling culturing method. The horseradish seedling culturing method comprises the following steps: (A) rooting cultivation, (B) hardening seedling transplanting and (C) filed planting, wherein the rooting cultivation comprises the step that primarily cultured stem tip meristem tissue which undergoes sterilization and detoxification is transferred into a 1 / 2 Murashige and Skoog (MS) culture medium to grow, the hardening seedling transplanting comprises the step of transferring the tissue into a tray after the step (A) lasts for 28-32 days, and the field planting comprises the step of transplanting finished horseradish seedlings to fields after the step (B) lasts for 75-85 days. The method of adding rooting agents into the culture medium for enrichment culture is adopted to replace the method of using cytokinin, so that the problems that rooting is slow and roots are thin and long are solved, and the purpose of increasing the length of the root of a single seedling quickly in a short period is achieved. At the same time, the price of the rooting agents is far lower than that of the cytokinin. Compared with the auxin culturing method which is large in waste quantity and low in use ratio, the horseradish seedling culturing method is smaller in dosage, higher in use ratio and capable of greatly reducing cost.

Description

Technical field [0001] The present invention relates to a seedling wasabi planting method in the agricultural field, and specifically refers to a factory wasabi planting method. Background technique [0002] In the development of my country's wasabi industry, Ink disease (also known as black heart disease) is the most important disease in wasabi planting, which can cause complete failure of wasabi planting in severe cases. Existing studies have found that Ink disease is a unique disease of wasabi, which does not cause harm to other crops, nor does it adversely affect human health. Ink disease is caused by Phoma wasabiae Yokogi (Phoma wasabiae Yokogi) invading the wasabi plant and infecting mainly through vascular bundles, which seriously affects the quality and yield of wasabi, and even causes the wasabi to fail. Ink disease can damage the rhizomes, fibrous roots, leaves and petioles of wasabi. After years of research in Japan, Taiwan, and the mainland, no drugs have been found...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G1/00
Inventor 方季虎
Owner 四川华阳蓝公府实业有限公司
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