Grape biological fresh-keeping agent and preparation method thereof

A technology for biological preservatives and grapes, which is applied in the fields of fruit and vegetable preservation, food preservation, food science, etc., can solve the problems of single strain of biological preservatives, short-term preservation expectations, and insignificant effects, and achieves low cost and product preparation. and ease of use, the effect of reducing the symptoms of decay

Inactive Publication Date: 2013-06-05
NANTONG ZHONGKE RUIZHI TECH SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are various fresh-keeping technical measures, chemical preservatives, and biological preservatives on the market, there are some problems. For example, the use of low-temperature preservation will be limited by facilities and conditions, and chemical preservatives are strictly controlled by the international community. Eating is also harmful to health. Biological preservatives have factors such as single strains, insufficient viable bacteria, short-term fresh-keeping expectations, and ineffective effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0015] Specific embodiment one: a kind of biological antistaling agent of grape is made of sodium chloride 1-8g, calcium chloride 1-6g, chitosan 3-9g, β-1,3-glucanase 1-10g, nisin Bacterin (concentration is 75 units / 150ul) 0.1-0.5, B-CHD bacillus fermentation broth 15-40g, 1L normal temperature sterile water, preparation method:

[0016] 1. Add sodium chloride 1-8g, calcium chloride 1-6g, chitosan 3-9g, β-1,3-glucanase 1-10g, nisin (concentration is 75 units / 150ul) Add 0.1-0.5g into 1L sterile water at room temperature and stir until the above substances are completely dissolved, then add 15-40g of B-CHD Bacillus fermentation broth and stir for 5-10min.

[0017] Using the antistaling agent of the present invention, the freshly picked grapes were sterilized by ultraviolet rays and immersed therein for 3 minutes, and stored in the same environment (natural environment at normal temperature) with untreated grapes (after the grapes were sterilized by ultraviolet rays). As a resul...

specific Embodiment approach 2

[0018] Specific embodiment two: adopt the formula of embodiment one, use biological antistaling agent 2% solution of the present invention to grape spraying 5 hours before picking grapes, treat that antistaling agent on the grapes dries up and then picks, whole fringe, cuts shriveled grain and Small fruit, put the processed grapes into fruit boxes lined with polyethylene film bags. Put the grape fruit box open in the mechanical cold storage for 24 hours. When the temperature of the fruit drops to 0°C, seal the film bag and cover the box cover, and store it at a temperature of 0-1°C, without spraying fresh-keeping The treated grapes are also whole ears, and the shriveled and small fruit grains are cut off, and the treated grapes are put into fruit boxes lined with polyethylene film bags. Put the grape fruit box open in the mechanical cold storage for 24 hours to pre-cool. When the fruit temperature drops to 0°C, seal the film bag, cover the box cover, and store it at a temperat...

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PUM

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Abstract

The invention relates to a grape biological fresh-keeping agent which comprises 1-8 g of sodium chloride, 1-6 g of calcium chloride, 3-9 g of chitosan, 0.1-0.5 g of nisin, 1-10 g of beta-1,3-glucanase, 15-40 g of B-CHD bacillus fermentation liquor, and 1 L of normal temperature sterile water; the preparation method comprises the following steps: adding 1-8 g of sodium chloride, 1-6 g of calcium chloride, 3-9 g of chitosan, 1-10 g of beta-1,3-glucanase, 0.1-0.5 g of nisin with a concentration unit of 75 units/150 ul into 1 L of normal temperature sterile water, stirring to completely dissolve; then adding 15-40 g of B-CHD bacillus fermentation liquor. The advantages of the invention are that the product is free of pollution, low in cost, safe, and nutritious, has multifunctional characteristics of obviously reducing grape fruit decay and threshing, preventing oxidation and coloration, and preventing fiber decomposition and softening, is suitable for storage and fresh keeping after grape picking, and is an economical and ideal biological fresh-keeping agent.

Description

Technical field [0001] The invention relates to a biological preservative for grapes and a preparation method thereof Background technique [0002] In recent years, due to the severe resistance of various postharvest pathogenic bacteria to chemical fungicides and the harm to the environment and human beings caused by long-term use of chemical fungicides, it is urgent for people to find new methods to control postharvest diseases of fruits and vegetables. The use of living microorganisms and metabolites for biological preservation has become a research hotspot. Yeast biocontrol living agents have obvious defects such as harsh environmental requirements, lagging action, and unstable effect, while microbial secondary metabolites have the advantages of large molecular weight, complex structure, and difficulty in producing resistance to pathogens; at the same time, secondary microbial The production cycle of secondary metabolites is short, and it is not restricted by seasons and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155A23B7/154
Inventor 朱益明
Owner NANTONG ZHONGKE RUIZHI TECH SERVICE
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