Preparation method for mulberry leaf polysaccharide hypoglycemic active component

A technology of mulberry leaf polysaccharides and active components, applied in the field of natural medicine extraction, can solve the problem of less research on hypoglycemic active components

Inactive Publication Date: 2013-06-05
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] At present, the research on mulberry leaf polysaccharides at home and abroad mostly focuses on the separation and purification of polysaccharides and the test of lowering blood sugar and lipids of polysaccharide crude extracts on diabetic animal models. less research

Method used

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  • Preparation method for mulberry leaf polysaccharide hypoglycemic active component
  • Preparation method for mulberry leaf polysaccharide hypoglycemic active component
  • Preparation method for mulberry leaf polysaccharide hypoglycemic active component

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Embodiment Construction

[0014] 1. The preparation of the hypoglycemic active component of mulberry leaf polysaccharide is carried out according to the following steps:

[0015] Fresh mulberry leaves are dried and crushed and passed through a 40-mesh sieve (1), weigh 100 g of mulberry leaf powder, wrap it in a filter paper bag and place it in a Soxhlet extractor, add 400 mL of petroleum ether, heat up to 80°C, and then condense and reflux Degrease 3 hours (2), take out the mulberry leaf powder of step (2) gained, rinse 3 times with 95% ethanol, each 3 minutes, mulberry leaf powder is through weighing and ethanol by the mass ratio of 1:8 during flushing, then Rinse 3 times with deionized water, 5 minutes each time, weigh the mulberry leaf powder and deionized water according to the mass ratio of 1:8, and then place it in hot water at 80°C for 4 times, 45 minutes each time (3), using a rotary evaporator to concentrate the extract, deproteinize 3 times with trichloroacetic acid, the concentration of tric...

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Abstract

The invention provides a preparation method for a mulberry leaf polysaccharide hypoglycemic active component, and belongs to the technical field of natural medicine extraction. After water extraction, deproteinization, ethanol extraction, and freeze drying are conducted to dry mulberry leaf powder of 100g, mulberry leaf total polysaccharides of 3.2896g are obtained, obtaining rate is 3.29%, and purity is 92.81%. The obtained polysaccharide sample respectively undergoes diethyl-aminoethanol (DEAE)-52 cellulose column chromatography and SephadexG-100 column chromatography purification so as to obtain three uniform polysaccharide single components, namely a mulberry leaf polysaccharide I (MLPI), an MLP II, and an MLP III. Each component of the mulberry leaf polysaccharide has good effect to weight-losing and improvement of hyperglycemia symptoms of a diabetic rat, can repair injury of pancreas islet, facilitates pancreatic beta cells of the diabetic rat to secrete insulin, is free of toxic and side effect, and can be used for treating diabetes mellitus.

Description

technical field [0001] The invention relates to the separation and extraction of an active component with hypoglycemic effect from mulberry leaves, which belongs to the technical field of natural medicine extraction. Background technique [0002] Moraceae (Morus Morus .L.) In addition to being used as feed for silkworms, the leaves of the plant also have the function of medicine and food. Since ancient times, traditional Chinese medicine has used mulberry leaves as a traditional Chinese medicine for treating diabetes (Institute of Materia Medica, Chinese Academy of Medical Sciences. Chinese Materia Medica [M ]. Beijing: People's Medical Publishing House, 1982). Modern pharmacological studies have shown that Mulberry leaf extract restores arterial pressure in streptozotocin-induced chronic diabetic rats[J]. Nutrition Research, 2009, 29: 602-608). At present, the hypoglycemic active ingredient of mulberry leaves that scholars at home and abroad pay the most attention to is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P3/10
Inventor 牟志美路国兵崔为正王彦文高绘菊张瑶
Owner SHANDONG AGRICULTURAL UNIVERSITY
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