Spirulina phatensis polysaccharide and cynomorium songaricum polysaccharide preparation and manufacture method thereof and application thereof
The technology of spirulina polysaccharide and Cynomorium polysaccharide is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effects of good taste, enhanced fatigue resistance, and improved smell and taste.
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experiment example 1
[0027] Experimental Example 1 Experiment on the inhibitory effect of different polysaccharides on tumor cells
[0028] Test cells: colon cancer SW620 cells and cervical cancer HeLa cells
[0029] Test materials: Spirulina polysaccharide, Cynomorium polysaccharide, Spirulina Cynomorium mixed polysaccharide (Spirulina polysaccharide and Cynomorium polysaccharide are added in equal proportions)
[0030] Experimental method: Take the tumor cells in the logarithmic growth phase, digest with trypsin, wash with Hank's solution, and adjust the cells to 1×10 with RPMI1640 medium containing 10% calf serum. 5 / ml. Add 100 μl of the above cell suspension to each well of a 96-well culture plate, under 5% CO. 2 After culturing in an incubator with a saturated humidity of 37°C for 24 hours, 100 μl of Cynomorium polysaccharide, Spirulina polysaccharide and Spirulina Cynomorium mixed polysaccharide of different concentrations prepared with RPMI1640 medium containing 10% calf serum were added...
Embodiment 1
[0034] 1) Formula of preparations
[0035] Spirulina polysaccharide 60mg / ml Cynomorium polysaccharide 80mg / ml
[0036] Sodium Carboxymethyl Cellulose 8mg / ml Citric Acid 3mg / ml
[0037] Honey 8 mg / ml Sodium Benzoate 0.1 mg / ml
[0038] 2) Production method
[0039]Weigh 8g of sodium carboxymethylcellulose and 3g of citric acid, add 440g of purified water, stir to dissolve; then add 60g of spirulina polysaccharide, 80g of Cynomorium polysaccharide and 0.1g of sodium benzoate, slowly stir to dissolve completely, add 8g of honey, After the solution is vacuum degassed, add purified water to make the volume to 1L, filter with a double filter, then bottle the oral liquid, lock the cap, and it is a product after passing the inspection.
Embodiment 2
[0041] The same as Example 1, the difference is: the spirulina polysaccharide in the formula is 80mg / ml Cynomorium polysaccharide is 80mg / ml.
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