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Enzyme method for extracting ginsenoside and obtained ginsenoside extractive

A ginsenoside and enzymatic extraction technology, applied in the directions of drug combinations, pharmaceutical formulations, plant raw materials, etc., can solve the problems of insufficient extraction of active ingredients, long extraction time, and complicated operations, and achieve full and comprehensive saponin components and high extraction rate. , the effect of simple operation

Active Publication Date: 2013-06-12
安徽丰润生物技术股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing methods for extracting ginsenosides from ginseng still have disadvantages such as cumbersome operation, long extraction time, low efficiency, insufficient extraction of active ingredients, and waste of medicinal materials.

Method used

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  • Enzyme method for extracting ginsenoside and obtained ginsenoside extractive
  • Enzyme method for extracting ginsenoside and obtained ginsenoside extractive
  • Enzyme method for extracting ginsenoside and obtained ginsenoside extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] See figure 1 As shown in the extraction process, the enzymatic extraction of ginsenosides in this embodiment is mainly carried out as follows:

[0040] Weigh 100g of ginseng medicinal material, soak overnight, cut into thin slices, add 400mL of water, add 50mg of cellulase, 100mg of pectinase, react at 45°C for 1 hour, then add 200mL of 95% ethanol to the enzymolysis extract to form The ethanol extract was refluxed at 55°C for 3 hours, and the extract was obtained by filtration; the residue was refluxed at 55°C for 2 hours with 800 mL of ethanol solution of the same concentration, and the extract was obtained by filtration, and the two ethanol extracts were combined. Concentrate the extract under reduced pressure at 50°C to form an extract, recover ethanol during the concentration process, and finally vacuum-dry the extract below 60°C to obtain a powder that is ginsenoside extract.

[0041] The ginsenoside extract of the present embodiment is black brown powder.

[00...

Embodiment 2

[0044] Weigh 100g of ginseng medicinal material, soak overnight, cut into thin slices, add 400mL of water, add 75mg of cellulase, 75mg of pectinase, react at 45°C for 1 hour, then add 200mL of 95% ethanol to the extract to form ethanol extraction solution, reflux at 55°C for 3 hours, and filter to obtain an extract; add 800 mL of ethanol solution of the same concentration to the residue, reflux at 55°C for 2 hours, filter to obtain an extract, and combine the two ethanol extracts. Concentrate the extract under reduced pressure at 50°C to form an extract, recover ethanol during the concentration process, and finally dry the extract under vacuum at a temperature below 60°C to obtain a powder that is the ginsenoside extract of this example. The yield of ginsenosides extracted by enzymatic method determined by colorimetry is 47.6% higher than that extracted by simple hot alcohol reflux method.

Embodiment 3

[0046] Weigh 100g of ginseng medicinal material, soak overnight, cut into thin slices, add 400mL of water, add 150mg of cellulase, react at 45°C for 1 hour, add 200mL of ethanol with a volume concentration of 95% to make an ethanol solution, reflux at 55°C for 3 hours, and filter to obtain Extract solution; add 800 mL of ethanol solution of the same concentration to the residue, reflux at 55°C for 2 hours, filter to obtain the extract solution, and combine the two ethanol extract solutions. The extract is concentrated under reduced pressure at 50°C to form an extract, ethanol is recovered during the concentration process, and finally the extract is further vacuum-dried below 60°C to obtain an extract that is ginsenoside extract. The yield of ginsenosides extracted by enzymatic method determined by colorimetry is 32.3% higher than that extracted by simple hot alcohol reflux method.

[0047] 1. Extraction method and grouping

[0048] Weigh four portions of 100g of ginseng medic...

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Abstract

The invention provides an enzyme method for extracting ginsenoside and an obtained ginsenoside extractive. The method comprises the steps of carrying out enzyme extraction and alcohol extraction on vegetable drug ginseng, wherein the enzyme extraction step is as follows: an aqueous solution with an enzyme preparation is taken as an enzyme extracting solution, the smashed or sliced ginseng is steeped and extracted, the enzyme preparation is cellulase, pectinase or a mixture of the cellulase and the pectinase, and enzymatic hydrolysis is carried out for 0.5-6 hours at 30-50 DEG C in an extraction process; the alcohol extraction step is as follows: alcohol is added to a ginseng solution subjected to enzymatic hydrolysis for preparing an alcohol extract with 30%-60% of volume concentration, and heating reflux is carried out for 2 hours to 10 hours at 50 DEG C-90 DEG C; and residues are refluxed and extracted again by using alcohol with the same concentration for 1-4 hours, and extracting solutions are combined. The ginsenoside extractive obtained by the method has high content of effective components; and the extraction rate is increased by above 30% relative to that of simple alcohol heating reflux. The method has the advantages of mild extraction conditions, operation simplicity and low cost.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting ginsenoside from ginseng by adopting biological enzyme technology and the extracted ginsenoside extract. Background technique [0002] Ginseng (Panax ginseng C.A.Mey) is the dried root of Araliaceae plant ginseng, commonly known as ginseng, ghost cover, goblin, baby ginseng, etc. It is a precious medicinal material in my country and has a medicinal history of thousands of years. Ginseng is mild in nature, sweet in taste, slightly bitter and lukewarm. It has the effects of invigorating vital energy, recovering pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and quenching thirst, calming the nerves and improving intelligence. In recent years, scholars at home and abroad have conducted pharmacological studies, clinical observations and epidemiological examinations on the active ingredient...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P29/00A61P7/02A61P25/00A61P25/28A61P39/06A61P9/10A61P9/06A61K125/00
Inventor 陈勇
Owner 安徽丰润生物技术股份有限公司
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