Manufacturing method of water-quality acute toxicity high throughput rapid determination reagent kit and using method thereof

Abstract

The invention relates to a manufacturing method of a water-quality acute toxicity high throughout rapid determination reagent kit and a using method of the water-quality toxicity high throughput rapid determine reagent kit. The manufacturing method of the toxicity high throughput rapid determination reagent kit comprises the following steps: (1) preparing photobacteria lyophilized powder; (2) configuring broth dilution water; (3) configuring phenol mother liquid; and (4) producing accessories and packing. The using method of the water-quality acute toxicity high throughout rapid determine reagent kit comprises the following steps: (1) reviving the bacteria lyophilized powder; (2) configuring standard solution of phenol series; (3) measuring residual chlorine; (4) measuring the potential hydrogen (pH) value; (5) preprocessing samples; (6) comparing and preparing dilution of the samples; (7) measuring accuracy of a standard curve of the phenol; (8) adding the photobacteria; (9) measuring light intensity; (10) calculating a result; (11) ensuring and controlling the quality; and (12) expressing the toxicity of the samples. The manufacturing method of the water-quality acute toxicity high throughput rapid determination reagent kit and the using method of the water-quality acute toxicity high throughput rapid determine reagent kit have the advantages of being applicable to rapid measurement of the water-quality toxicity, perfect in manufacturing and using, low in cost, and rapid and accurate in measurement.

Description

technical field [0001] The invention relates to the technical field of a kit, in particular to a method for making and using a kit for measuring the acute toxicity of water substances by using freshwater luminescent bacteria. Background technique [0002] At present, "Water and Wastewater Monitoring and Analysis Methods" (Fourth Edition) provides a method for evaluating water quality toxicity using freshwater Vibrio Qinghai, which requires the use of standard toxic substance phenol (solvent is 10% lactose solution) to test the luminescent bacteria before the test. Quality testing to ensure that the luminescent bacteria respond accurately to water quality toxicity in a good state, and 10% lactose must also be added to the sample to be tested. This method has the following problems in the test process: the first, the 10% lactose solution is unstable, and the phenol mother liquor configured with 10% lactose can only be stored for 2 days at most in a refrigerator at 4 ° C, and s...

AI Technical Summary

Problems solved by technology

This method has the following problems in the test process: the first, the 10% lactose solution is unstable, and the phenol mother liquor configured with 10% lactose can only be stored for 2 days at most in a refrigerator at 4 ° C, and should be measured immediately after configuration; and 100ml Only 63.5ml and 18ml of the 1000mg / L mother solution and 450ml serial standard solutions were used during the experiment, so that the phenol solution could not be fully used, causing a lot of waste, and the collected phenol waste solution increased harmful substances The cost and difficulty of disposal may cause serious pollution to the environment

Second, 10% lactose is difficult to dissolve naturally after being added to the sample, and heating is required during the dissolution process, which will change the properties of the sample and affect the accuracy of the results

Third, since the luminous intensity of luminescent bacteria will change with time, when using a conventional photometer to detect a single light intensity, it is necessary to measure a control for one sample, which is time-consuming and labor-intensive, and increases the cost.

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