Nondestructive measurement method of chlorophyll in leaf

A measurement method, chlorophyll technology, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of non-quantifiable, limited leaf thickness, low resolution, etc., and achieve the effect of eliminating influence and reducing error

Inactive Publication Date: 2013-06-19
河南农大迅捷测试技术有限公司
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Problems solved by technology

[0004] ①The measured leaf thickness is limited, and the content concentration is limited: this is because the leaf is a detection object with very low transparency; according to the SPAD manual, its maximum measurable thickness is 1.2mm, in fact, when the chlorophyll concentration is high, its resol...

Method used

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  • Nondestructive measurement method of chlorophyll in leaf
  • Nondestructive measurement method of chlorophyll in leaf
  • Nondestructive measurement method of chlorophyll in leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0063] Embodiment 1: first method (2 wavelength method) of the present invention measures the chlorophyll content in the paulownia leaf

[0064] (1) The selected working wavelength is 620nm, and the reference wavelength is 960nm;

[0065] (2) The monochromatic light of the working wavelength and the reference wavelength is sequentially irradiated on the blade to be tested and the reference reflector (the whiteboard is used as the reference reflector, and the reflectivity is 100%)) to cause diffuse reflection, and photoelectric reflection The instrument detects and records the diffuse reflectance R at two wavelengths 工 , R 参1 (the results are shown in Table 1), the following formula obtains the chlorophyll reflectance:

[0066] R=R 工 -r 1 × R 参1

[0067] (3) Substituting the chlorophyll reflectance R obtained in the previous step into the following Kubilka-Munck formula to calculate the reflectance function F of the chlorophyll contained in the leaves to be tested KM (re...

Embodiment 2

[0075] Embodiment 2 first method (2 wavelength method) of the present invention measures the chlorophyll content in the rose leaf

[0076] The detection method is basically the same as in Example 1, except that the selected detection wavelengths are different: the working wavelength is 650nm, and the reference wavelength is 940nm; the relevant detection and calculation results are shown in Table 2.

[0077] The conversion equation for the chlorophyll content in the leaves to be measured is:

[0078] C=0.4478×F KM +0.0456, r=0.834, the correlation is extremely significant.

[0079] Table 2 Relevant measurement parameters and calculation parameters in the detection process of chlorophyll in rose leaves

[0080]

[0081] n=9, r=0.834 (r 0.05＝ 0.602, r 0.01 = 0.735).

Embodiment 3

[0082] Embodiment 3: second method (3 wavelength method) of the present invention measures rape leaf

[0083] (1) The first wavelength is 640nm, the second wavelength is 660 and the reference wavelength is 1000nm;

[0084] (2) The monochromatic light of the first working wavelength, the second working wavelength and the reference wavelength are respectively irradiated on the blade to be tested and the reference reflector (the whiteboard is used as the reference reflector, and the reflectance is 100%) to generate Diffuse reflectance, detect and record the diffuse reflectance R at three wavelengths λ１ , R λ２ , R 参2 , get the reflectance R of chlorophyll a by the following formula a and the reflectance R of chlorophyll b b :

[0085] R a =R λ2 -r 2 × R 参2 ,

[0086] R b =R λ1 -r 3 × R 参2 ,

[0087] where r 2 、r 3 is a coefficient, and takes a value of 0.9 to 1.8;

[0088] (3) Calculate the chlorophyll reflectance function: the reflectance R of chlorophyll a and ...

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Abstract

The invention relates to a nondestructive measurement method of chlorophyll in a leaf. The method comprises the steps of irradiating the leaf and a reflector plate by rays with two (or three) specific wavelengths, and calculating a relative index (or actual content) of chlorophyll content according to the reflectivity, wherein the index (or actual content) and the total content of chlorophylls (or content of chlorophyll a, content of chlorophyll b, and total content) have obvious correlativity (or quantitative functional relation), so as to estimate and calculate or restore the content of chlorophylls (or calculating chlorophyll a, chlorophyll b and total chlorophyll content) in the leaf according to the correlation. The method disclosed by the invention is not limited by the thickness of the leaf while the error is rapidly amplified by the existing transmission-type colorimetric assay method when the thickness of the leaf is large and the absorptivity A is greater than 1.0. According to the scattered reflection measurement method disclosed by the invention, diffuse reflection is carried out at any level inside the leaf, and the effect of the thickness of the leaf is small. The method disclosed by the invention is low in detection cost of the chlorophyll; no consumable items are used; and the technical preparation process of a conversion model is not needed.

Description

technical field [0001] The invention relates to a detection and analysis method for chlorophyll, in particular to a method for non-destructive determination of chlorophyll in leaves. Background technique [0002] Chlorophyll content is an important indicator to measure plant photosynthesis and growth status. The research on chlorophyll content detection technology is of vital significance in agricultural production and forestry research. The traditional detection method of chlorophyll content is to extract chlorophyll first, and then measure its content by spectrophotometry. This method has many disadvantages, such as time-consuming, destructive to blades, inconvenient for field measurement, and inconvenient for continuous measurement. Now, the chlorophyll measuring instrument is widely used for measurement. Although this method makes up for the shortcomings of the traditional measurement method and can quickly and accurately measure the chlorophyll content without damage, ...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 段铁城胡建东胡小宁杨张青苏伟波叶优良王万章朱秀红刘存祥管泽民
Owner 河南农大迅捷测试技术有限公司
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