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Method for extracting maslinic acid and oleanolic acid containing mixture from olea europaea L. pomace

A technology of olive fruit and oleanolic acid, which is applied in the direction of medical preparations containing active ingredients, food science, drug combination, etc., can solve the problems of no commercial supply, complicated extraction process, high production cost, etc., and achieve green reagents Environmental protection, simple process route and low production cost

Active Publication Date: 2013-06-26
TIANYUAN OIL OLIVE TECH DEV LONGMAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this process has potential industrial value, it has not yet formed a commercial supply due to the complex extraction process and high production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh 20 g of shade-dried olive pomace, put it in a 500 mL round bottom flask, add 240 mL of 80% ethanol, extract in a water bath under normal pressure for 1 h, and pour out the extract. Add 200mL of 80% ethanol to the dregs, extract under normal pressure reflux for 1h, pour out the extract, and discard the dregs. The two extracts were combined and centrifuged. Concentrate the supernatant under reduced pressure to near dryness, add 50% ethanol aqueous solution to dissolve and set the volume to 250mL, so that the concentration is equivalent to 0.08Kg of olive pomace per liter, and mix well to obtain the sample solution.

[0021] Weigh 7 g of the macroporous adsorption resin, and wet-load it into a glass column with an inner diameter of 1.0 cm and a column height of 12 cm (diameter-to-height ratio 1:12) (1BV=4 mL). Take 100mL of the sample solution and put it on the column for adsorption, once at a flow rate of 15BV / h, and discard the adsorption residue. Add 10BV of wat...

Embodiment 2

[0023] Weigh 1 Kg of shade-dried olive pomace, put it into a multi-effect extraction tank, add 10 L of 95% ethanol to extract for 1.5 h, and pour out the extract. Add 8 L of 95% ethanol to the dregs to extract for 1.5 h, pour out the extract, and discard the dregs. The two extracts were combined and centrifuged. After the supernatant was concentrated under reduced pressure to recover ethanol, 70% ethanol was added to make the volume up to 5L, so that the concentration was equivalent to 0.2Kg of olive pomace per liter, and mixed well to obtain the sample solution. Weigh 8Kg of macroporous adsorption resin, and put it into a resin column (1BV=0.98L) with an inner diameter of 5.0cm and a column height of 50cm (diameter-to-height ratio 1:10) by wet method. Take 4L of the sample solution and put it on the column, absorb it at a flow rate of 20BV / h, put the effluent solution on the column for re-adsorption, cycle the adsorption twice, and discard the adsorption residue. Add 10BV o...

Embodiment 3

[0025] Change the desorption solution to be acetone, and other conditions are the same as in Example 1 to obtain the extract extract, and use high performance liquid chromatography (HPLC) to measure the maslinic acid content of 33.4%, and the oleanolic acid content of 25.5%.

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PUM

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Abstract

The invention discloses a method for extracting a maslinic acid and oleanolic acid containing mixture from olea europaea L. pomace. The method comprises the following steps of: extracting an extract from the olea europaea L. pomace in an 80-95wt% (weight percent) ethanol aqueous solution, concentrating the extract, redissolving the extract in a 20-70wt% ethanol aqueous solution, adsorbing by using a macroporous adsorption resin, removing water-soluble impurities by using a water-washing resin column, desorbing by using a desorbing solution, mixing the desorbing solution, recovering the desorbing solution, and drying to obtain a maslinic acid and oleanolic acid containing extract. The mixture extracted from the olea europaea L. pomace contains more than 30% of maslinic acid and more than 23% of oleanolic acid.

Description

technical field [0001] The invention relates to a method for extracting a mixture containing maslinic acid and oleanolic acid from olive pomace. Background technique [0002] (Olea europaea L.) is a broad-leaved plant of the genus Oleaceae Oleaceae, also known as foreign olive. It is a world-renowned tree species for woody oil and fruit. There are 15 species distributed in China, mainly distributed in Gansu, Yunnan, Sichuan, Guizhou, etc. land. Planting olives is mainly to use the oil in the fruit to process olive oil. However, after the olives are processed to obtain olive oil with high nutritional value, the pomace has not been well utilized in our country. The pomace is mostly discarded directly. Not only seriously pollute the environment, but also cause a waste of resources to a certain extent. Therefore, it is very necessary to comprehensively develop and utilize olive pomace. [0003] Olive pomace is composed of olive peel, fruit stone and residual pulp, which main...

Claims

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Application Information

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IPC IPC(8): A61K36/63A61P31/04A61P29/00A61P3/10A61P3/06A61P31/18A61P35/00A61P39/06A23L1/29A61K131/00A23L33/00
Inventor 邸多隆黄新异王着
Owner TIANYUAN OIL OLIVE TECH DEV LONGMAN
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