Method for improving inductive generation efficiency of induced pluripotent stem cells

A technology of pluripotent stem cells and stem cells, applied in the field of improving the efficiency of induction of pluripotent stem cells, can solve the problems of low induction efficiency and achieve the effect of improving efficiency, quality and high induction efficiency
CN103173490BInactive Publication Date: 2015-01-07GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Publication Date
2015-01-07
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a method for improving the inductive generation efficiency of induced pluripotent stem cells, and especially relates to a method for improving the inductive generation efficiency of the induced pluripotent stem cells through using modified histone gene jhdm1a. Jhdm1a and a stem cell induction factor are utilized to improve the induction efficiency and the induction quality of the induced pluripotent stem cells. The stem cell induction factor can be an Oct4 and Klf4 combination, or an Sox2, Oct4 and Klf4 combination, or an Oct4 and Sox2 combination, or individual Oct4. The method also comprises a step that the cells are exposed to vitamin C, so the induction efficiency of the induced pluripotent stem cells is higher than that of methods which do not use vitamin C. The method uses less stem cell induction factor, so the latent carcinogenicity is reduced, a high induction efficiency is obtained, and the induced pluripotent stem cells having a reproduction system transmission capability and a high quality can also be obtained.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] The present invention is a divisional application of a Chinese invention patent application with the patent application number 201110323779.7 and the filing date of October 21, 2011, entitled "A Method for Improving the Efficiency of Inducing Pluripotent Stem Cells". technical field

[0002] The invention relates to a method for improving the efficiency of inducing and generating pluripotent stem cells. In particular, it relates to a method for improving the efficiency of inducing and generating pluripotent stem cells by modifying histone genes jhdm1b and jhdm1a. Background technique

[0003] my country is a country with a large population in the world. Every year, organ defects, failures, and dysfunctions caused by trauma, disease, aging, and genetics also rank first in the world. Therefore, the research on stem cells and regenerative medicine has attracted the general attention of quite a few scientific research institutes and all walks of life.

[0004] Cell transp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More