Unlock instant, AI-driven research and patent intelligence for your innovation.

Enzyme linked immunosorbent assay kit for detecting 21 sulfonamides and 11 quinolones and application of enzyme linked immunosorbent assay kit

An enzyme-linked immunosorbent reagent and drug technology, which is applied in the directions of microorganism-based methods, immunoglobulins, biochemical equipment and methods, etc., to achieve the effects of simple sample pretreatment process, strong practicability, and simple and easy testing methods.

Active Publication Date: 2013-06-26
CHINA AGRI UNIV
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the sulfonamide drug residue detection kit mainly focuses on the detection of 3 to 5 sulfonamide drugs, and the quinolone drug residue detection kit mainly focuses on the detection of 5 to 10 quinolones. ELISA kits for various sulfonamides and quinolones

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enzyme linked immunosorbent assay kit for detecting 21 sulfonamides and 11 quinolones and application of enzyme linked immunosorbent assay kit
  • Enzyme linked immunosorbent assay kit for detecting 21 sulfonamides and 11 quinolones and application of enzyme linked immunosorbent assay kit
  • Enzyme linked immunosorbent assay kit for detecting 21 sulfonamides and 11 quinolones and application of enzyme linked immunosorbent assay kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the preparation and application of the enzyme-linked immunosorbent assay kit that detects sulfa drug and / or quinolone drug

[0065] 1. Preparation of an enzyme-linked immunosorbent assay kit for detecting sulfonamides and / or quinolones

[0066] ELISA kits for the detection of sulfonamides and / or quinolones include:

[0067] (1) Pre-coated ELISA plate: ELISA plate coated with the original coating (the conjugate of sulfamethazine and carrier protein, and the conjugate of norfloxacin and carrier protein);

[0068] (2) Primary antibody: anti-sulfamethazine antibody working solution, and anti-norfloxacin antibody working solution, that is, anti-sulfamethazine antibody and anti-norfloxacin antibody are respectively diluted with antibody (0.02M phosphate buffer containing 0.05% Triton X-100 and 0.02% Proclin300) diluted to a protein concentration of 0.1 μg / mL.

[0069] (3) Enzyme-labeled secondary antibody: Alkaline phosphatase-labeled goat anti-mouse secondary...

Embodiment 2

[0152] Example 2, the ELISA kit precision, accuracy, specificity and shelf life experiments prepared in Example 1

[0153] In this example, the precision, accuracy, specificity, and shelf life of the enzyme-linked immunosorbent kit prepared in Example 1 will be tested. For the use method and result analysis of the kit involved, please refer to Step 2 of Example 1. step.

[0154] 1. Kit precision experiment

[0155] 1. Standard product precision test

[0156] Get three batches from the enzyme-linked immunosorbent assay kit prepared in embodiment 1 and carry out precision test, each batch respectively extracts 10 kits, each plate each extracts 20 microwells, measures 20 μ g / L standard solution (sulfamethazine standard product and ciprofloxacin standard solution, both concentrations are 20μg / L) absorbance value, calculate the coefficient of variation (CV%), the results are shown in Table 1.

[0157] Table 1 Standard precision test results (CV%)

[0158]

[0159] From the t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an enzyme linked immunosorbent assay kit for detecting 21 sulfonamides and 11 quinolones and application of the enzyme linked immunosorbent assay kit. The enzyme linked immunosorbent assay kit comprises an antibody 1, an antibody 2, a coating antigen 1, a coating antigen 2, an enzyme marker 1 and an enzyme marker, wherein the antibody 1 resists sulfonamides, the antibody 2 resists quinolones, the coating antigen 1 is a conjugate of sulfonamides haptens and carrier proteins, the coating antigen 1 is a conjugate of quinolones haptens and carrier proteins, the enzyme marker 1 is an enzyme-labeled secondary antibody resisting the antibody 1, and the enzyme marker 2 is an enzyme-labeled secondary antibody resisting the antibody 2. The enzyme linked immunosorbent assay kit provided by the invention is capable of simultaneously qualitatively or quantitatively detecting residues of 21 sulfonamides or 11 quinolones in a sample to be detected, and is simple in pretreatment process of the sample. A method is simple and easy to operate, and high in practicability, sensitivity and accuracy.

Description

technical field [0001] The invention relates to an enzyme-linked immunoassay kit for detecting sulfa drugs and / or quinolone drugs and application thereof. Background technique [0002] Sulfonamides are artificially synthesized sulfonamide derivatives, mainly used for the prevention and treatment of bacterial infectious diseases. Representative drugs are: sulfamethazine, sulfadiazine, sulfamethoxazole, sulfamethoxine, sulfaquinoxaline, etc. Sulfonamide drugs can inhibit Gram-positive bacteria and some negative bacteria, and can treat a variety of bacterial infections. They are widely used in veterinary clinics to treat various livestock and poultry diseases infected by sensitive bacteria. [0003] Quinolones (4-quinolones) are a relatively new class of synthetic antibacterial drugs, representative of which are ofloxacin, pefloxacin, enoxacin, ciprofloxacin, etc. Most of the molecules of this class of drugs contain fluorine atoms, so they are called fluoroquinolones. Quinol...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/566G01N33/577C07K16/44C12N5/20C12R1/91
Inventor 沈建忠王战辉蒋文晓史为民曹兴元张素霞李建成江海洋丁双阳
Owner CHINA AGRI UNIV