Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for measuring clobetasol propionate in products used for promoting growth and development of hairs

A technology of clobetasol propionate and products, which is applied in the field of determination of clobetasol propionate in hair growth and development products, can solve problems such as hidden dangers to consumers' health and safety, allergic reactions, and low entry barriers, and achieves good results. Economic and social benefits, simple pre-processing, and high accuracy

Active Publication Date: 2013-07-10
GUANGZHOU QUALITY SUPERVISION & TESTING INST
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the low production technology content of the hair growth cosmetics industry, the low entry barriers to the industry, and the lack of corporate awareness of brand intellectual property rights and product quality and safety, the current hair growth cosmetics industry is in chaos. The main problems are as follows : 1. The product quality is uneven, and a large number of fake and inferior products are flooding the market
2. False and excessive advertising is serious
3. Illegal addition of prohibited drugs
[0005] However, due to the outstanding anti-inflammatory effect of clobetasol propionate, it is often compounded with ketoconazole, minoxidil and other drugs, and is illegally added to growth and development products
Such drugs stay on the skin, face, hair and other parts for a long time, which may cause allergic reactions or toxicity, and bring serious hidden dangers to the health and safety of consumers. Western medicine ingredients listed as banned substances

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring clobetasol propionate in products used for promoting growth and development of hairs
  • Method for measuring clobetasol propionate in products used for promoting growth and development of hairs
  • Method for measuring clobetasol propionate in products used for promoting growth and development of hairs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The establishment of clobetasol propionate reference substance chromatogram

[0030] Include the following steps:

[0031] (1) Preparation of clobetasol propionate standard solution

[0032] Using the mobile phase solution (acetonitrile / water = 1:1) as the solvent, prepare concentrations of 0.1mg / L, 0.2mg / L, 0.5mg / L, 2.0mg / L, 5.0mg / L, 10.0mg / L, 20.0mg / L clobetasol propionate standard solution.

[0033] (2) Ultra-high performance liquid chromatography detection

[0034] Precisely draw 5 μL of the standard solution from low to high concentration, and perform ultra-high performance liquid chromatography detection in sequence, the conditions are as follows:

[0035] Column: BEH C 18 (2.1x50mm, 1.7μm) column (Waters);

[0036] Column temperature: 40°C;

[0037] Mobile phase: acetonitrile and water, gradient elution, the program is: 0-1.5min, maintain 50% acetonitrile, 1.5-1.8min, the volume of acetonitrile increases from 50% to 70%; 1.8-2.5min, the volume ...

Embodiment 2

[0045] Example 2 Determination of Clobetasol Propionate Content in Hair Tonic Sample

[0046] Include the following steps:

[0047] (1) Preparation of test samples

[0048] Accurately weigh 0.5-1.0g (accurate to 0.001g) sample, place it in a 10mL colorimetric tube, add 3mL each of acetonitrile, saturated sodium chloride solution and n-hexane accurately in sequence, then fully homogenize on a homogenizer, freeze Centrifuge, use a needle to extract the clarified liquid in the middle layer, and transfer to a 10mL centrifuge tube. Add 3 mL of acetonitrile to the original colorimetric tube, and repeat the above steps. Combine the two extracted clarified solutions, add 0.2mL of 10% potassium ferrocyanide solution (v / v), then add 0.2mL of 20% zinc acetate solution (v / v), dilute to 10mL with water, mix well, and centrifuge , and the supernatant was filtered through a 0.45 μm filter membrane for UPLC determination.

[0049] (2) Determination by ultra-high performance liquid chromat...

Embodiment 3

[0059] Example 3 Determination of the content of clobetasol propionate in the hair growth liquid sample with a concentration of 4.0 mg / kg clobetasol propionate

[0060] Include the following steps:

[0061] (1) Preparation of test samples

[0062] Accurately take by weighing 0.5g of hair tonic sample in embodiment 2, add concentration in this sample and be 100 μ L of clobetasol propionate standard solution of 20.0mg / L, then the concentration of clobetasol propionate in this hair tonic sample is 4.0 mg / kg. Subsequent processing was the same as in Example 2 to obtain a test sample.

[0063] (2) Determination by ultra-high performance liquid chromatography

[0064] With embodiment 2.

[0065] (3) Determination

[0066] The obtained chromatogram is as Figure 4 shown. According to the sample quality represented by the test sample, the content of clobetasol propionate in the test sample is calculated from the standard curve of Example 1. The calculated content of clobetasol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for measuring clobetasol propionate in products used for promoting the growth and the development of hairs; the method comprises the steps of: preparing a sample to be tested; carrying out ultra-high performance liquid chromatography measurement on the sample to be tested; adopting a BEH C18 (2.1*50mm, 1.7mu m) chromatographic column with the column temperature of 35-45 DEG C; taking acetonitrile and water as moving phases, and carrying out gradient elution; controlling the constant flow speed to be 0.3-0.4mL / min; and taking Waters personal digital assistant (PDA) as a detector, wherein the detection wavelength is 230-240nm. After being used for detecting the content of the clobetasol propionate in the products used for promoting the growth and the development of the hairs, the method is low in detection limit, high in degree of accuracy, simple in pretreatment and very suitable for the rapid batch detection of the clobetasol propionate in the products used for promoting the growth and the development of the hairs; and the method provides the important technical support for the quality control and safety monitoring of cosmetic products, and has good economic and social benefits.

Description

technical field [0001] The invention belongs to the field of cosmetic analysis, in particular, the invention relates to a method for measuring clobetasol propionate in hair growth and development products. Background technique [0002] With the development of society and economy, the pace of people's life, work and study is constantly accelerating, people's psychological pressure is increasing, and the incidence of hair loss in the crowd is also increasing. Congenital alopecia, androgenetic alopecia, alopecia areata and other symptoms increasingly common occurrence. Hair loss is becoming more and more common, and hair growth and hair growth products for hair loss are emerging in the market. Advertisements for this type of product can be seen everywhere on the market, on TV, and on the Internet. [0003] However, due to the low production technology content of the hair growth cosmetics industry, the low entry barriers to the industry, and the lack of corporate awareness of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 席绍峰李慧勇王继才谭建华
Owner GUANGZHOU QUALITY SUPERVISION & TESTING INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com