Method for determining benzo[a]pyrene in main stream smoke total particulate matters of cigarette

Abstract

The invention discloses a method for determining benzo[a]pyrene in main stream smoke total particulate matters of a cigarette, which comprises the following steps: (1) preparing a cured tobacco or blended type cigarette sample according to GB / T19609, balancing, smoking through a smoking machine, and collecting smoke total particulate matters of the cigarette on a Cambridge filter disc; (2) taking out the Cambridge filter disc, putting into a conical flask, adding a deuterated internal standard, and extracting benzo[a]pyrene in the smoke particulate matters of the cigarette by using a mixed solution of tetrahydrofuran and acetonitrile (1:10) as solvent; and (3) passing the filtrate obtained after extraction through an organic filter membrane, putting into a chromatographic vial, analyzing through UPLC-APPI-MS / MS, performing MRM mode scanning, and performing quantitative determination through a deuterated internal standard method. The method is simple in pretreatment, favorable in reproducibility and high in analysis sensitivity, and can perform accurate qualitative and quantitative analysis on benzo[a]pyrene in the smoke particulate matters of the cigarette.

Description

Technical field: [0001] The invention relates to a method for measuring benzo[a]pyrene in the total particulate matter of cigarette mainstream smoke, and belongs to the technical field of methods for measuring chemical components of tobacco. Background technique: [0002] Cigarettes are burned under the condition of high temperature and lack of oxygen, which will produce a variety of condensed ring aromatic hydrocarbons, about 200 of which have been identified so far, of which benzo[a]pyrene is the representative compound with the strongest carcinogenicity. Therefore, measuring the content of benzo[a]pyrene in mainstream cigarette smoke is of great significance to the research on smoking and health. [0003] The determination of benzo[a]pyrene in mainstream cigarette smoke in foreign countries began in the 1960s. At present, the methods for determining benzo[a]pyrene mainly include fluorescence spectrophotometry, gas chromatography, high performance liquid chromatography and...

AI Technical Summary

Problems solved by technology

However, there are two problems in these methods: 1) The pretreatment process of the sample is complicated, usually through the steps of extraction, concentration, purification, and reconcentration. The complicated pretreatment process is time-consuming and reduces the analysis efficiency on the one hand. It will inevitably bring more links and factors that cause experimental errors, and the parallelism of the experimental results is poor; 2) The detection limit and sensitivity are poor, because the content of benzo[a]pyrene in cigarettes is at the level of ng level, and the content of cigarette smoke There are thousands of substances in the granular phase. In such a complex background, it is difficult for conventional chromatographic analysis instruments to accurately analyze them qualitatively and quantitatively, including gas chromatography-mass spectrometry. The samples have only a small response signal and are prone to interference; 3) The analysis time is long, because there are thousands of components in the particulate matter of cigarette smoke, and the prerequisite for accurate qualitative and quantitative analysis is a good resolution, so usually The analysis time is long, such as the current commonly used GC / MS method, each sample takes about an hour

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