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Storage method of lily powder

A technology for lily pollen and pollen, which is applied in the directions of botanical equipment and methods, plant preservation, application, etc., can solve the problems of high preservation cost, complicated and cumbersome procedures, and short lifespan maintenance time of lily pollen, and can ensure uniformity. and stability, constant humidity effect and stable effect

Inactive Publication Date: 2013-08-14
YANGZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. At temperatures such as 5°C and -18°C, the lifespan of lily pollen is relatively short, only 30-80 days, and the length of pollen lifespan is unstable, which cannot meet the requirements of breeders for the anniversary or more of lily pollen. long-term storage requirements
[0006] 2. Storage at ultra-low temperature requires ultra-low temperature refrigerators or liquid nitrogen, and the cost of storage is high. When using it, the stored pollen must be awakened gradually at different temperatures, and the procedure is complicated and cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1) Configuration of constant humidity salt solution - sodium nitrate saturated solution

[0024] Take 100ml of pure water, add 110g of sodium nitrite to it, and heat to dissolve.

[0025] 2) Take 200ml of the prepared solution and add it to the bottom of a glass desiccator with an inner diameter of 100mm (GB / T15723-1995), until the liquid level is 1-2cm away from the porcelain plate of the desiccator, and seal it for later use;

[0026] 3) Select lily flowers that bloom on the day, collect pollen with a soft brush, and put the collected pollen into 00# medical gelatin hollow hard capsules (standard YBX-2000-2007 of China Pharmaceutical Packaging Association), and the amount of each capsule should not exceed 20mg , After covering the capsule cover, use a needle to pierce a small hole at both ends of the capsule, with a diameter of 0.5-0.7mm, to facilitate gas exchange.

[0027] 4) Put the pollen-filled capsule into an envelope or paper bag, seal it with a paper clip and...

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PUM

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Abstract

The invention discloses a storage method of lily powder. The storage method comprises the following steps of: (1), preparing a constant-humidity salt solution-sodium nitrite saturated solution; (2), adding the prepared solution to the bottom of a dryer until the liquid surface is 1-2cm away from the porcelain plate of the dryer, sealing for later use, wherein the relative humidity is 60%-70%; (3), filling collected pollen to a gelatin hollow capsule, covering the capsule cover, and puncturing a pore at each of the two ends of the capsule respectively for facilitating gas exchange; (4), placing the capsule filled with the pollen to an envelope or a paper bag, using a paper clip for sealing and placing on the porcelain plate of the dryer, wherein the sealing by a paper clip aims at facilitating gas exchange; and (5), sealing the dryer and placing to a refrigerator of 5 DEG C for storage for a long time. The storage method of the lily powder is simple and easy to implement; and the service life of the lily powder stored by the method can achieve one year or longer.

Description

technical field [0001] The invention relates to a method for storing lily pollen. Background technique [0002] Pollen is the microspore of a flowering plant. When it germinates, it produces a male gametophyte containing three haploid nuclei, which contains all the genetic information of the plant, and is an important material for plant hybrid breeding and germplasm resource preservation. Pollen viability refers to the ability of pollen to survive, grow, germinate or develop, and is one of the key factors for the success of hybrid breeding. Pollen lifespan is the period during which pollen remains viable under certain conditions. [0003] Lily (Lilium spp.) is an important bulbous flower. The natural lifespan of lily pollen is short, and cross breeding often encounters problems such as parental flowering and long-distance hybridization. Breeders need to solve the above problems through pollen storage; in addition, pollen storage is also an important technology for the pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N3/00
Inventor 高红胜陆志新路广黄金福马文高新年
Owner YANGZHOU UNIV
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