Rhodamine 6G-based Cu<2+> fluorescence probe molecule and preparation method thereof
A technology of fluorescent probe and rhodamine hydrazide, which is applied in the field of metal ion analysis and detection, achieves the effects of simple preparation method, strong selectivity and high reaction efficiency
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[0029] Example 1 Fluorescent probe molecules of the invention L Synthetic method:
[0030] Step 1: Rhodamine 6G hydrazide is synthesized according to the method of J. AM. CHEM. SOC. 2005, 127, 16760-16761.
[0031] Step 2: Add rhodamine hydrazide (214.0mg, 0.5mmol) and methyl 3-aldehyde-2-hydroxybenzoate (90.1mg, 0.5mmol) to 10ml of methanol solution. After mixing well, add a few drops of glacial acetic acid . Stir and reflux at 68°C for 2h, cool to room temperature, a light pink precipitate is formed, filtered, and the precipitate is washed several times with a 1:1 mixed solution of methanol and ether and then dried in vacuum. The obtained solid was 253.8 mg, and the yield was 86%.
[0032]
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[0033] Example 2 The fluorescent probe molecule of the present invention L is Application experiments in detecting copper ions:
[0034] Prepare 5mM aqueous solutions of various metal salts with deionized water. Weigh 2.95mg of the fluorescent probe molecule of the present invention L, Dissolve it in 25ml of N,N-2-methylformamide solution (DMF) to prepare a 0.2mM solution. Add 3ml of N,N-2-methylformamide to the cuvette, and then add 75μL of 0.2mM of the above-configured fluorescent probe molecule of the invention to it L Dilute it to 5μM. After mixing uniformly, use 521nm as the excitation wavelength and measure the fluorescence value in the range of 538nm-700nm.
[0035] The fluorescent probe molecule of the present invention L Selectivity: 5μM fluorescent probe molecule of the invention L There is only weak fluorescence at 559nm. Add 10 equivalents of various metal ions to the system, as shown figure 1 As shown, only the addition of copper ions caused a significant fluorescen...
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