Method for efficiently degrading lignocelluloses raw material

A technology of lignocellulose and cellulase, which is applied in the direction of fermentation, can solve the problems of reducing production cost and energy consumption, reducing the crystallinity of cellulose raw materials, and shortening the pretreatment time, so as to shorten the pretreatment time and improve the pretreatment time. Treatment effect, effect of relieving environmental stress

Active Publication Date: 2013-08-14
HENAN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

[0006] The purpose of the present invention is to provide a high-efficiency degradation of lignocellulosic raw materials in view of the limitations of long pretreatment time, high production cost, large energy consumption and low enzymatic hydrolysis efficiency of the current lignocellulosic material pretreatment technology. method, in the pretreatment process, the cationic surfactant cetyltrimethylammonium bromide is assisted, which significantly improves the pretreatment efficiency, shortens the pretreatment time, reduces the production cost and energy consumption, and Effectively reduce the crystallinity of cellulose raw materials, adding non-ionic surfactants in the enzymatic hydrolysis process of lignocellulosic raw materials can significantly improve the enzymatic hydrolysis effect of cellulase

Method used

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  • Method for efficiently degrading lignocelluloses raw material
  • Method for efficiently degrading lignocelluloses raw material

Examples

Experimental program
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Embodiment 1

[0032] Take 0.08g of cetyltrimethylammonium bromide, add 25mL of NaOH solution with a mass fraction of 1.75%, stir until it is completely dissolved, add 1g of raw material of peanut shell powder, and then under normal pressure and microwave power of 640W, Heat treatment for 19 minutes, then filter and separate, wash the filter residue with tap water until the filtrate is neutral, and dry at 65°C; take 0.5g of the residue, add 10mg of cellulase and 5mg of PEG6000, and then add 0.1mol / L lemon with a pH of 4.8 Acid-sodium citrate buffer 25mL was hydrolyzed for 36h at a water bath temperature of 45°C and a speed of 160r / min, and then centrifuged at a speed of 5000r / min for 10min, using 3,5-dinitrosalicylic acid method for the determination of reducing sugar content in the supernatant. The results are shown in Table 1

Embodiment 2

[0034]Take 0.10g of cetyltrimethylammonium bromide, add 25mL of NaOH solution with a mass fraction of 1.75%, stir until it is completely dissolved, add 1g of raw material of peanut shell powder, and then under normal pressure and microwave power of 640W, Heat treatment for 22 minutes, then filter and separate, wash the filter residue with tap water until the filtrate is neutral, and dry at 65°C; take 0.5g of the residue, add 10mg of cellulase and 10mg of PEG6000, add 0.1mol / L citric acid with a pH of 4.8 - Sodium citrate buffer solution 25mL, under the condition of water bath temperature 45℃, rotation speed 160r / min, hydrolyze for 48h, then centrifuge 10min under the condition of rotation speed 5000r / min, use 3,5-dinitrosalicylic acid method The reducing sugar content in the supernatant was determined. The results are shown in Table 1

Embodiment 3

[0036] Take 0.08g of cetyltrimethylammonium bromide, add 25mL of NaOH solution with a mass fraction of 1.75%, stir until it is completely dissolved, add 1g of raw material of peanut shell powder, and then under normal pressure and microwave power of 640W, Heat treatment for 22 minutes, then filter and separate, wash the filter residue with tap water until the filtrate is neutral, and dry at 65°C; take 0.5g of the residue, add 10mg of cellulase and 10mg of PEG6000, add 0.1mol / L citric acid with a pH of 4.8 - Sodium citrate buffer solution 25mL, under the condition of water bath temperature 45℃, rotation speed 160r / min, hydrolyze for 48h, then centrifuge 10min under the condition of rotation speed 5000r / min, use 3,5-dinitrosalicylic acid method The reducing sugar content in the supernatant was determined. The results are shown in Table 1

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Abstract

The invention discloses a method for efficiently degrading lignocelluloses raw material. The method comprises the following steps of: simple smashing of raw material as well as enzymolysis of normal pressure microwave unite alkali liquor and cationic surface active agent disposal raw material and nonionic surfactant auxiliary cellulose, wherein the roughly smashed lignocelluloses raw material, the cationic surface active agent and the alkali liquor are mixed, after microwave processing at normal temperature, liquid and solid are separated, and furthermore, the nonionic surfactant auxiliary cellulose is added for solid enzymolysis so as to obtain a sugar liquid. According to the method, the advantages of the physical method and the chemical pretreatment method are complementary, so that the degree of crystallinity of the cellulose raw material is effectively lowered, the pretreatment time is shortened, and the pretreatment effect is improved; furthermore, alkali liquor with low concentration is beneficial to effective recycle in industrial production, pretreatment of lignocelluloses raw material at normal temperature can also lower the energy consumption in the pretreatment process obviously, and sugar liquid with higher concentration can be obtained in shorter hydrolysis time as the nonionic surfactant auxiliary cellulose is used for enzymolysis.

Description

technical field [0001] The invention belongs to the field of lignocellulosic material pretreatment technology and its application and the field of new energy development, and specifically relates to a method for efficiently degrading lignocellulosic raw materials. Background technique [0002] my country is one of the countries with the fastest economic development in the world. The demand for energy has continued to grow at a high speed for a long time. With the increasing energy gap in the world, the depletion of non-renewable energy such as fossil energy and the increasing environmental pressure, countries around the world have also accelerated the search for The pace of alternative energy, among which bioethanol has attracted the attention of countries all over the world for its resource sustainability, technical feasibility and environmental friendliness. Lignocellulose can be saccharified to obtain fermented sugar solution, which is then used to ferment industrial produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 陈复生方志锋刘昆仑王洪杰张丽芬郭珍李蒙李彦磊王萌蕾李润洁李文高艳秀陈亚敏杨颖莹杨趁仙朱婷伟刘少博杨光胜王书展
Owner HENAN UNIVERSITY OF TECHNOLOGY
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