Method for quantifying fibrinolytic active ingredients in animal medicines
A technology of active ingredients and animal medicinal materials, applied in the field of quantification of fibrinolytic active ingredients, can solve the problems of restricting the use of animal medicines and the inability to accurately provide animal medicines, and achieve remarkable effects and simple processes
Inactive Publication Date: 2013-08-14
JIANGSU TIANZHAO PHARMA
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Problems solved by technology
Modern Chinese medicine has not been able to accurately provide the content determination method in the finished preparations of animal drugs (such as ants, ground beetles), which greatly limits the use of animal drugs. This patent introduces the method of in vitro protein activity determination into the process of drug content determination for the first time , providing a new method for the quality research and production control of the same type of preparation
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0012] Take an appropriate amount of bovine fibrinogen, grind it finely, weigh it accurately, use borax buffer solution (take 32.42g of sodium tetraborate and 5.84g of sodium chloride, add 500ml of distilled water, heat to dissolve, adjust the pH value to 7.8 with 7mol / L hydrochloric acid ) made into a solution containing 2mg per 1ml, that is. Take a sterilized petri dish, add 10ml of fibrinogen solution, quickly add 0.5ml of thrombin solution (made with the above borax buffer solution containing 40 units of bovine thrombin per 1ml), mix well, and let it stand at room temperature for 10 minutes. Fibrin standard plate. Take an appropriate amount of urokinase reference substance, weigh it accurately, and use sterile water to make a solution containing 8U per 1ml as the reference substance solution.
[0013] Take an appropriate amount of the content of this product, weigh it accurately, make a solution containing 30 mg per 1 ml with sterile water, shake for 20 minutes, centrifug...
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The invention relates to a method for quantifying fibrinolytic active ingredients in animal medicines. The method comprises the following steps of: (1) taking a proper amount of bovine blood fibrinogen, grinding into fine powder, accurately weighing, and preparing a solution which contains 2mg of fine powder per ml by using a borax buffer solution; (2) taking disinfected culture dishes, respectively adding 10ml of fibrinogen solution, quickly adding 0.5ml of thrombin solution, uniformly mixing, and standing for 10 minutes at room temperature; (3) taking a proper amount of urokinase reference substance, accurately weighing, and preparing a solution which contains 8U of urokinase reference substance per ml by using sterilized water to be used as a reference substance solution; (4) taking a proper amount of content, accurately weighing, preparing a solution which contains 30mg of content per ml by using sterilized water, shaking for 20 minutes, centrifuging, and taking supernatant liquid to be used as a test solution; and (5) taking a fibrin plate, respectively sucking and dropping 10mu l of reference substance solution and test solution on the fibrin plate, putting the fibrin plate in an electrothermostat at 37 DEG C for 18 hours, taking out the fibrin plate, respectively measuring the long diameter and short diameter of the plaque of each point, and then calculating the quantity of the fibrinolytic active ingredients according to a molten ring area.
Description
technical field [0001] The invention relates to a method for quantifying fibrinolytic active ingredients in animal medicinal materials such as ants and ground beetles. technical background [0002] The fibrinolytic system refers to the reaction system in which plasminogen is transformed into plasmin by the action of specific activators, leading to the continuous dissolution of fibrin in the body, mainly including plasminogen, plasmin, plasminogen activator and plasminogen. Lysogen activator inhibitors. [0003] Liver fibrosis is a repair response of the liver to chronic damage caused by various factors, characterized by excessive deposition of extracellular matrix (ECM) in the liver, and the degradation of ECM is regulated by various factors. Recent studies have shown that The plasminogen activation system can participate in the degradation process of ECM by regulating the activity of matrix metalloproteinases (MMPs). [0004] Plasmin can directly degrade fibrinogen and fi...
Claims
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IPC IPC(8): C12Q1/56
Inventor 乐智勇夏培元刘任刘丽玲辛小娜张兵兵
Owner JIANGSU TIANZHAO PHARMA