Method for predicating metabolizable energy level of pig diets by metabonomics technology

A metabolizable energy value and equation technology, which is applied in the field of predicting the metabolic energy level of pig diets, can solve the problems of slow progress, inability to operate recipes, and lack of establishment of dynamic regression prediction equations for plasma metabolic markers, so as to improve pig production efficiency. Effect

Active Publication Date: 2013-08-14
NINGBO TECH BANK
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  • Application Information

AI Technical Summary

Problems solved by technology

Due to the particularity of the research objects, artificial recipe manipulation is not possible, and the identification of markers relies on clinical nutrition investigation and analysis, and the progress is very slow
[0006] So far, there is no report on the use of metabolomics data to establish a dynamic regression prediction equation between plasma metabolic markers and dietary exergy values ​​(metabolizable energy, digestible energy, and net energy, etc.) to accurately predict the dietary exergy value of piglets

Method used

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  • Method for predicating metabolizable energy level of pig diets by metabonomics technology
  • Method for predicating metabolizable energy level of pig diets by metabonomics technology
  • Method for predicating metabolizable energy level of pig diets by metabonomics technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1. Effects of Different Dietary Metabolizable Energy Levels on Piglet Growth Performance

[0056] 1. Experimental animals

[0057] A total of 180 healthy Duroc×Landrace×Large White ternary growing pigs were selected for the test, with half male and half female. The average weaning age was 28 days, and the test officially started after one week of transition. The initial body weight of the experiment was 13.4±2.1kg. Based on the initial body weight, it was divided into 5 treatments according to the randomized block experimental design, each treatment had 6 repetitions, each pen was a repetition, and each repetition was 6 pigs. Male and female are kept separately.

[0058] 2. Feeding management

[0059] The all-in and all-out feeding management mode is adopted, the temperature of the pig house is controlled at 24-27°C, and the light program is 12h light / 12h dark. Each circle (1.5×3.0m 2 ) are equipped with 1 nipple drinker and 2 feeding troughs. Net bed feedi...

Embodiment 2

[0080] Example 2, Screening of dietary energy markers based on plasma metabolome profile

[0081] 1. Sample processing

[0082] The isolated piglet plasma sample in Example 1 was taken out from the -80°C refrigerator and then thawed on ice. Take 100 μL of plasma from each part, add 400 μL of metabolite extraction solution (mixed with methanol and acetonitrile at a volume ratio of 1:1), vortex and shake for 5 minutes, extract at -20°C for 1 hour, and centrifuge at 4°C and 13,000 rpm 10min. Carefully pipette 200 μL of the supernatant into a new centrifuge tube, blow dry with nitrogen at room temperature, and redissolve in 200 μL of 80% methanol solution. After shaking for 5 s, centrifuge again to take the supernatant, put it into a sample bottle, and perform high-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (HPLC Q-TOF MS) detection.

[0083] 2. Conditions for HPLC Q-TOF MS detection

[0084] Instruments: HPLC Q-TOF MS detection system...

Embodiment 3

[0114] Example 3. Establishment of the Prediction Equation for the Metabolizable Energy Level of Weaned Piglets' Diets

[0115] The purpose of this example is to find out the metabolic markers associated with production performance and nutritional parameters and their changing rules through the analysis of plasma metabolic end products, and to explore how to determine the metabolites in the plasma of weaned piglets fed different levels of metabolizable energy. Markers predict the effective energy level of piglets, establish a dynamic regression prediction equation between plasma metabolic markers and effective energy value, and try to accurately predict the effective energy value of piglets through only one or several plasma metabolic markers. It has important theoretical and practical significance to meet the nutritional needs of pigs under different environmental, physiological and pathological conditions, and to improve pig production efficiency.

[0116] 1. Relative conten...

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Abstract

The invention discloses a method for predicating the metabolizable energy level of pig diets by a metabonomics technology. The method is characterized in that the relative content of one to seven of eight plasma metabolic markers of a pig to be tested is plugged in equations with one to seven unknown quantities established in the invention to obtain the predicted value of metabolizable energy of the diets of the pig to be tested, wherein the plasma metabolic markers include lysophosphatidylcholine (18:3/0:0), myristoyl lysophosphatidylcholine (14:0/0:0), glycerophosphorylethanolamine (18:0/0:0), lysophosphatidylcholine (20:4/20:4), proline, phosphatidylcholine (18:2/0:0), arginylphenylalanylarginine and glycerophosphorylethanolamine (18:1(9Z)/0:0), and the relative deviation between the predicted value obtained from the equation with seven unknown quantities and an actual value does not exceed +/-1 percent. The method has significant theoretical and practical significances for evaluating the nutritional status and optimizing the formula in time, meeting the nutritional requirements of pigs under physiological and pathological conditions in different environments and improving the production efficiency of pigs.

Description

technical field [0001] The invention relates to a method for predicting the metabolizable energy level of pig diets by using metabolomics technology. Background technique [0002] Energy is an important nutrient component of feed. Animals' nutritional needs or nutritional supply are based on energy, and energy feed also occupies the largest proportion of feed cost. In recent years, due to the application of corn and other raw materials in the production of industrial alcohol and other aspects, there has been a shortage of energy feed and the price has become more and more expensive. Therefore, how to rationally utilize energy in feed, which not only meets the energy needs of piglets, but also avoids the waste of energy feed, is particularly important. [0003] In the current intensive pig production, the normal turnover of pigs on the production line is usually achieved according to the all-in-all-out process, and the same batch of pigs is fed with the same level of metabol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王军军林刚臧建军王晓秋李德发李溱戴兆来
Owner NINGBO TECH BANK
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