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Method for extracting total alkaloids from fritillaria pallidiflora

A technology of total alkaloids and Igbemo, which is applied in the field of medicine and can solve problems such as related reports that have not yet been seen

Inactive Publication Date: 2013-08-21
乌鲁木齐欧易生物医学科技有限公司
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  • Claims
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AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the enrichment and preparation process of the total alkaloids of I.

Method used

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Embodiment Construction

[0022] 1) Single factor, orthogonal test design screening:

[0023] Taking the content of total alkaloids and the extraction rate as the investigation indicators, the single factors such as pH value, reflux time, reflux times, and ethanol volume fraction that affect the extraction efficiency of total alkaloids of I. Factor Three Level L 9 (3 4 ) to conduct an orthogonal test to seek the optimum extraction process conditions for the total alkaloids of I. japonicus.

[0024] The optimal extraction process conditions for the total alkaloids of I. fritillaria are as follows: take an appropriate amount of I. fritillaria powder and pass through an 80-mesh sieve, add 8 to 14 times the amount of 40 to 80% acidic ethanol with a pH value of 1 to 4, and reflux extraction 2 to 4 times, 30 to 90 minutes each time, filter, combine the filtrates, recover under reduced pressure until there is no alcohol smell, and obtain the extract;

[0025] The above extract was basified with 5% sodium h...

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Abstract

The invention discloses a method for extracting total alkaloids from fritillaria pallidiflora, which comprises the following steps of: drying and grinding fritillaria pallidiflora; adding acid ethanol and performing reflux extraction for 2-4 times; filtering and performing reduced-pressure recovery until ethanol smell disappears; performing alkalization; respectively letting NaCl and distilled water pass through any macroporous adsorption resin of AB-8, HPD450 and X-5 to remove inorganic salt and water-soluble impurities; respectively letting 30-50% ethanol solution and 60-90% ethanol solution pass through the macroporous adsorption resin to elute off alkaloids adsorbed on the resin; collecting the elution liquid and performing reduced-pressure recovery and concentrating to obtain extract; and drying at 40-60 DEG C for 72 hours to obtain the total alkaloids of fritillaria pallidiflora. The method disclosed by the invention avoids the problem that a traditional preparation method utilizes organic solvents such as petroleum ether, chloroform and the like, has the advantages of high content of total alkaloids, high extraction efficiency and reasonable and feasible technology, shortens the production period and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting total alkaloids from Iberia japonicus, belonging to the technical field of medicine. Background technique [0002] It is the dried bulb of Fritillaria pallidiflora or F.walujewii, a plant of Liliaceae. It has the effects of clearing away heat and nourishing the lungs, reducing phlegm and relieving cough. It is comparable to the medicinal material Chuan Fritillaria in terms of clinical application. Its wild species only grows in Xinjiang, China. At present, the large-scale plantation of Iberia japonicus has become an important way to boost the income of local farmers and herdsmen in Xinjiang. It is a characteristic medicinal material in Xinjiang. [0003] In recent years, pharmacists have focused on the extraction and separation of chemical components, pharmacological activity and content determination in the research on Ignebum in recent years. Its main components are isosteroid alkaloids and glycoside...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8966
Inventor 张超杨志华陈慕芝
Owner 乌鲁木齐欧易生物医学科技有限公司
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