Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue

A technology for test strips and secreted proteins, which is used in biological tests, material inspection products, etc., to achieve the effects of simple operation, good stability, and intuitive and reliable result judgment.

Inactive Publication Date: 2013-08-21
SHANGHAI TENTH PEOPLES HOSPITAL
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no reports on medical devices for early diagnosis of tongue cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue
  • Application of CRISP (cysteine-rich secretory protein)1 kit for diagnosing carcinoma of tongue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Immunohistochemistry

[0017] Experimental materials: normal tongue tissue, tongue cancer tissue; the primary antibody is goat anti-human CRISP1 antibody, and the second antibody is donkey anti-goat Dylight594 antibody.

[0018] experimental method:

[0019] Tissue chip is to combine a large number of tissue samples orderly on the surface of a tiny substrate, and use immunohistochemistry for detection.

[0020] 1. Fix the tissue with PFA fixative for 5 minutes and wash with PBS for 10 minutes

[0021] 2. Antigen retrieval: Expose the epitope with 0.01M citrate solution. Heat the repair solution in a microwave on high heat for 3 minutes until it boils (4 minutes), and then continue to microwave for 1 minute or twice on low heat. Cool to room temperature and wash with PBS for 10 minutes. Break the membrane with 0.5% Triton for 10 minutes.

[0022] 3. Block non-specific proteins

[0023] 1) Soak in PBS for 3 minutes × 3 times

[0024] 2) 3% H 2 O 2 -Methanol (30% H 2 O 2 1...

Embodiment 2

[0036] Example 2 Enzyme-linked immunosorbent assay (Elisa)

[0037] A total of 90 cases, including 30 cases of tongue cancer (squamous cell carcinoma) group; 30 cases of other oral diseases (periodontal disease, pulp disease, oral candidiasis) group; 30 cases of healthy control group. Use the cysteine-rich secreted protein detection kit for detection, and the detection steps refer to the instruction manual of the cysteine-rich secreted protein quantitative enzyme-linked detection kit.

[0038] ELISA test results of CRISP1:

[0039] 1. The tongue cancer group is higher than the other two groups, while the other disease group has no difference with the healthy group (P=0.802).

[0040] Table 1 The diagnostic value of saliva CRISP1 in the diagnosis of tongue cancer

[0041] Diagnostic indicators AUC Sensitivity Specificity False positive rate False negative rate Youden Index Urine CRISP1 10 ng / ml0.809 88.1% 39.3% 60.7% 11.9%0.274 Urine CRISP1 40 ng / ml0.809 71.4% 87.1% 12.9% 29.6% 0...

Embodiment 3

[0044] Example 3 Preparation of gold-labeled antibody complex of test paper

[0045] 1. Preparation of colloidal gold

[0046] Add 100ml 0.005%-0.02% chloroauric acid solution into a round bottom flask and heat to boiling. After boiling, add freshly prepared 0.4%-2% trisodium citrate (Na 3 C 6 H 5 O 7 ·2H 2 O) 1-2.2ml aqueous solution, boil for 10-25 minutes, continue to stir and cool to room temperature. During this process, the color change of the solution can be seen: golden yellow→black→purple→dark blue→bright red. When the color of the solution is completely changed to transparent bright red, the required colloidal gold is obtained. After cooling, put it into a dialysis bag and dialyze the ultrapure water (1:5000) three times. Finally, transfer the dialyzed colloidal gold to a clean glass bottle with a screw cap, and store it at 4°C in a dark environment.

[0047] 2. The connection between gold colloid and protein

[0048] 1. The adsorption of colloidal gold to protein mainly de...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an application of CRISP (cysteine-rich secretory protein)1 test paper for diagnosing carcinoma of tongue to preparation of a medical instrument for diagnosing carcinoma of tongue. The test paper is characterized in that a gold colloid pad contains a gold-labelled anti-saliva CRISP1 antibody complex formed by binding colloidal gold and an anti-saliva CRISP1 antibody; the diagnosis sample of the test paper is saliva; and the detection threshold of the test paper is 10-40ng / ml, the concentration of the saliva CRISP1. The test paper is quick in checking, has high sensitivity, strong specificity and good stability, is simple and convenient to operate, dispenses with any instrument, is intuitive and reliable in result judgment, is easy to master and is simultaneously convenient for patients to self-monitor the conditions.

Description

Technical field [0001] The present invention relates to the application of a kit, in particular to the application of a CRISP1 kit for diagnosing tongue cancer. Background technique [0002] Early tongue cancer can be manifested as ulcer, exogenous and infiltrating three types. In some cases, the first symptom is only tongue pain, which can sometimes reflect to the temple or ears. The exophytic type can come from malignant transformation of papilloma. There may be no protrusions or ulcers on the infiltrating surface. Ulcer and infiltrating cancers are often accompanied by spontaneous pain and varying degrees of tongue movement limitation; exogenous general tongue movement disorders are not obvious and less spontaneous pain. In the advanced stage, tongue cancer can directly transcend the midline or invade the floor of the mouth, or infiltrate the lingual periosteum, bone plate or bone of the mandible. Later, it can extend to the base of the tongue or the anterior pharyngeal co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
Inventor 朱进安
Owner SHANGHAI TENTH PEOPLES HOSPITAL