Boron nitride nanosheet/gold nanocluster composite material and its preparation and its application in biological analysis

A gold nanocluster and composite material technology, which is applied to boron nitride nanosheet/gold nanocluster composite material and its preparation method and application field in biological analysis, can solve problems such as application that have not been reported in literature, and achieve water solubility. Low, reproducible, and stable effects

Inactive Publication Date: 2015-09-02
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As far as we know, there is no literature report on the boron nitride nanosheet / gold nanocluster composite material and its preparation and application in bioanalysis

Method used

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  • Boron nitride nanosheet/gold nanocluster composite material and its preparation and its application in biological analysis
  • Boron nitride nanosheet/gold nanocluster composite material and its preparation and its application in biological analysis
  • Boron nitride nanosheet/gold nanocluster composite material and its preparation and its application in biological analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Embodiment 1. Preparation of the composite material of boron nitride nanosheet / gold nanocluster

[0024] Weigh 100 milligrams of hexagonal boron nitride powder, disperse in 50 milliliters of diethylene glycol diacrylate (PDDA, concentration is 1 milligram per milliliter) in the aqueous solution of phthalate, pass through probe type ultrasonic instrument (VC-750, 20 kHz, 750W, 30% amplification, US Sonics&Materials company) ultrasonic stripping for 4 hours. After the dispersion was left for 24 hours, centrifuge at 2000 rpm for 20 minutes, take 40 ml of the supernatant for later use, use 10 ml of the above supernatant and centrifuge at 10,000 rpm for 10 minutes, and take 1 ml of the lower layer , washed 3 times with secondary water to obtain boron nitride nanosheets (concentration of 1 ± 0.1 mg per ml). Disperse 1 ml of the above-prepared boron nitride nanosheets in 2-3 ml of gold nanocluster solution [Preparation method of gold nanocluster solution: 0.5 ml of HAuCl with ...

Embodiment 2

[0025] Embodiment 2. The preparation of the composite material of boron nitride nanosheet / gold nanocluster

[0026] Change "100 mg of hexagonal boron nitride powder" in Example 1 to "200 mg of hexagonal boron nitride powder", and the other conditions of preparation are the same as in Example 1, and the results are the same as in Example 1.

Embodiment 3

[0027] Embodiment 3. Preparation of the composite material of boron nitride nanosheets / gold nanoclusters

[0028] "Dispersed in the aqueous solution of 50 milliliters of diethylene glycol diacrylate of phthalate" of embodiment 1 was changed into "in the aqueous solution of 100 milliliters of diethylene glycol diacrylate of phthalate", prepared Other conditions are with embodiment 1, and result is with embodiment 1.

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Abstract

The invention discloses a boron nitride nano sheet / gold nano cluster composite material. The boron nitride nano sheet / gold nano cluster composite material is prepared through the following steps of: executing probe type ultrasonic stripping on hexagonal boron nitride in a water solution (PDDA) of phthalic acid diethylene glycol diacrylate for 4 hours, after standing the dispersion liquid for 24 hours, centrifuging the dispersion liquid for 20 minutes at the speed of 2000rpm (revolutions per minute), and obtaining the supernatant liquid for use; obtaining 10ml of the supernatant liquid, further centrifuging for 10 minutes at the speed of 10000rpm, obtaining the subnatant, centrifugally washing the subnatant for 3 times with the secondary water, and then, getting the boron nitride nano sheet; and then, mixing the boron nitride nano sheet with the gold nano cluster under self-assembly action, performing ultrasonic treatment for 30 minutes, centrifuging for 10 minutes at the speed of 10000rpm, obtaining the subnatant, and centrifugally washing the subnatant for 3 times with the secondary water, thus, getting the boron nitride nano sheet / gold nano cluster composite material. The composite material provided by the invention has stronger yellow fluorescence, has lower toxicity to HeLa cell, and can be used in detection analysis, such as bio-labeling.

Description

technical field [0001] The invention relates to a boron nitride nanosheet / gold nanocluster composite material, a preparation method thereof and an application in biological analysis. Background technique [0002] In recent years, gold nanoclusters have aroused great interest of scientific researchers. Due to quantum confinement and edge effects, gold nanoclusters exhibit many novel chemical and physical properties [see: (a) A.Lechtken, D.Schooss, J.R.Stairs, M.N.Blom, F.Furche, N.Morgner, O .Kostko, B.von Issendorff and M.M.Kappes, Angew.Chem.Int.Ed.46(2007), 2944.(b)B.S.Gonzalez, M.J.Rodriguez, C.Blanco, J.Rivas, M.A.Lopez-Quintela and J.M.G.Martinho , Nano Lett 10 (2010), 4217.]. In addition, it has low cytotoxicity, excellent water solubility, chemical inertness, fluorescence stability, better surface grafting and other characteristics, which make it have broad application prospects in optoelectronic devices, sensors, bioimaging and other fields [see: (c) T.H.Lee, J.I....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/63G01N21/64C12Q1/02B82Y30/00
Inventor 朱俊杰杨国海王圣冉那
Owner NANJING UNIV
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