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Low-temperature biogas leavening agent and preparation method thereof

A technology of biogas fermentation and microbial agent, which is applied in the field of low-temperature biogas fermentation agent and its preparation, can solve the problems of long initial start-up time for biogas production, lack of methanogenic bacteria in biogas tanks, poor gas production stability, etc., and achieve rapid start-up and good fermentation. Quick start of fermentation promotion and low production cost

Active Publication Date: 2013-08-28
江苏新申奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] A Chinese patent for a methanogenic compound bacterial agent and its preparation method, the patent number is 200910187435.0 discloses the following content: a methanogenic compound bacterial agent and its preparation method solves the problem of the lack of efficient methanogenic bacteria in existing biogas digesters, which leads to The long-standing problem of biogas initial start-up time and poor gas production stability, it adopts the Methanosarcina acetophilus DSM-No.2834, Methanobacillus forminum DSM-No. Bacillus DSM-No.1125, Methanolobus Tyndallus DSM-No.2278, and Methanolobus kenzii DSM-No.6752 were inoculated in liquid methanogen culture medium under anaerobic conditions, and were separately expanded and cultivated step by step. The final methanogen bacteria solution is mixed together according to the volume ratio, which is the liquid methanogenic composite bacterial agent; the liquid methanogenic composite bacterial agent can also be prepared into a solid methanogenic composite bacterial agent
[0006] The above-mentioned patented technologies all disclose different preparation methods of bacterial agents, but they all have the problems of low efficiency and slow response in special winter environments.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A composite biogas fermentation bacterial agent, the composite biogas fermentation bacterial agent includes mixed microorganisms, antioxidants and protective agent carriers, the composition of mixed microorganisms in parts by weight is as follows: Methanococcus mazei DSM101328, Methanobacterium subterraneum DSM110747, Methanogenium frigidum DSM1645815, Methanosarcina acetivorans DSM28348, Methanosarcina siciliae, DSM119168, Methanosaeta harundinacea DSM-1720610;

[0030] The strains were all purchased from the German Culture Collection of Microorganisms, located in Braunschweig, Germany;

[0031] The antioxidant is formulated according to the mass percentage of the following components: 0.5% of cysteine ​​hydrochloride, 5% of sodium sulfide, 3% of ascorbic acid, and the balance is sand.

[0032] The protective agent carrier is formed by proportioning the following ingredients: 0.5% of sandy soil, 2% of bentonite, 5% of diatomaceous earth, 20% of cornstarch, and the rest...

Embodiment 2

[0043] A composite biogas fermentation bacterial agent, the composite biogas fermentation bacterial agent includes mixed microorganisms, antioxidants and protective agent carriers, the composition of mixed microorganisms in parts by weight is as follows: Methanococcus mazei DSM101325, Methanobacterium subterraneum DSM110746, Methanogenium frigidum DSM1645810, Methanosarcina acetivorans DSM28345, Methanosarcina siciliae, DSM1191610, Methanosaeta harundinacea DSM-1720614;

[0044] The antioxidant is formulated according to the mass percentage of the following components: 0.5% of cysteine ​​hydrochloride, 5% of sodium sulfide, 3% of ascorbic acid, and the balance is sand.

[0045] The protective agent carrier is formed by proportioning the following ingredients: 0.5% of sandy soil, 2% of bentonite, 5% of diatomaceous earth, 20% of cornstarch, and the rest is crushed straw.

example 1

[0047] 1 Cultivation of biogas fermentation bacteria: Methanococcus mazei (Methanococcus mazei), Methanobacterium subterraneum (subterranean methanobacillus), Methanosarcina siciliae (Methanosarcina siciliae, Methanosaeta harundinacea), respectively from the inclined plane Inoculate into the medium and culture at 22°C for 25 hours, then culture at 6°C for 86 hours at low temperature, remove the methane in the upper layer of the medium, and fill with carbon dioxide. Obtain biogas-related microbial fermentation liquid;

[0048] Inoculate Methanogenium frigidum (cold methanogens) and Methanosarcina acetivorans (acidophilic methane sarcini) into the culture medium from the slant respectively. After cultivating at 21°C for 24 hours, adjust the temperature after culturing at 2-4°C for 30 hours Continue culturing at 21°C for 40 hours, then adjust the temperature to 3°C for 18 hours, remove methane in the upper layer of the medium, and fill with carbon dioxide. Obtain biogas-related m...

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PUM

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Abstract

The technical problem to be solved in the invention is to overcome the defects in the prior art, so the invention provides a compound microorganism bacterium agent preparation, preservation and application technology through which biogas can be efficiently generated under the low-temperature condition. Water can be added into the compound bacterium agent and the mixture is directly added into a rural biogas digester after being uniformly mixed with low-temperature cellulose, so that the winter biogas can be kept stable. Besides, the invention further provides a compound bacterium agent preparation, preservation and application method.

Description

technical field [0001] The invention relates to a low-temperature biogas fermentation agent and a preparation method thereof, in particular to various low-temperature biogas fermentation microorganisms and their cultivation, preparation and storage methods, belonging to the field of agricultural technology. Background technique [0002] The state issued the "Agricultural Biomass Energy Industry Development Plan (2007-2015)", which focused on the development of rural biogas. Since the implementation of the rural biogas project, the state has invested 5 billion funds in the construction of rural biogas infrastructure. However, due to the long winter in the north, the low gas production of biogas digesters has plagued the development of the industry. Generally, the gas production of biogas digesters in winter accounts for about 10% of the annual gas production. 3%, so increasing the gas production in winter is a must to improve the utilization rate of biogas energy. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12R1/01
CPCY02E50/30
Inventor 马凯
Owner 江苏新申奥生物科技有限公司
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