Low-temperature biogas leavening agent and preparation method thereof
A technology of biogas fermentation and microbial agent, which is applied in the field of low-temperature biogas fermentation agent and its preparation, can solve the problems of long initial start-up time for biogas production, lack of methanogenic bacteria in biogas tanks, poor gas production stability, etc., and achieve rapid start-up and good fermentation. Quick start of fermentation promotion and low production cost
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Embodiment 1
[0029] A composite biogas fermentation bacterial agent, the composite biogas fermentation bacterial agent includes mixed microorganisms, antioxidants and protective agent carriers, the composition of mixed microorganisms in parts by weight is as follows: Methanococcus mazei DSM101328, Methanobacterium subterraneum DSM110747, Methanogenium frigidum DSM1645815, Methanosarcina acetivorans DSM28348, Methanosarcina siciliae, DSM119168, Methanosaeta harundinacea DSM-1720610;
[0030] The strains were all purchased from the German Culture Collection of Microorganisms, located in Braunschweig, Germany;
[0031] The antioxidant is formulated according to the mass percentage of the following components: 0.5% of cysteine hydrochloride, 5% of sodium sulfide, 3% of ascorbic acid, and the balance is sand.
[0032] The protective agent carrier is formed by proportioning the following ingredients: 0.5% of sandy soil, 2% of bentonite, 5% of diatomaceous earth, 20% of cornstarch, and the rest...
Embodiment 2
[0043] A composite biogas fermentation bacterial agent, the composite biogas fermentation bacterial agent includes mixed microorganisms, antioxidants and protective agent carriers, the composition of mixed microorganisms in parts by weight is as follows: Methanococcus mazei DSM101325, Methanobacterium subterraneum DSM110746, Methanogenium frigidum DSM1645810, Methanosarcina acetivorans DSM28345, Methanosarcina siciliae, DSM1191610, Methanosaeta harundinacea DSM-1720614;
[0044] The antioxidant is formulated according to the mass percentage of the following components: 0.5% of cysteine hydrochloride, 5% of sodium sulfide, 3% of ascorbic acid, and the balance is sand.
[0045] The protective agent carrier is formed by proportioning the following ingredients: 0.5% of sandy soil, 2% of bentonite, 5% of diatomaceous earth, 20% of cornstarch, and the rest is crushed straw.
example 1
[0047] 1 Cultivation of biogas fermentation bacteria: Methanococcus mazei (Methanococcus mazei), Methanobacterium subterraneum (subterranean methanobacillus), Methanosarcina siciliae (Methanosarcina siciliae, Methanosaeta harundinacea), respectively from the inclined plane Inoculate into the medium and culture at 22°C for 25 hours, then culture at 6°C for 86 hours at low temperature, remove the methane in the upper layer of the medium, and fill with carbon dioxide. Obtain biogas-related microbial fermentation liquid;
[0048] Inoculate Methanogenium frigidum (cold methanogens) and Methanosarcina acetivorans (acidophilic methane sarcini) into the culture medium from the slant respectively. After cultivating at 21°C for 24 hours, adjust the temperature after culturing at 2-4°C for 30 hours Continue culturing at 21°C for 40 hours, then adjust the temperature to 3°C for 18 hours, remove methane in the upper layer of the medium, and fill with carbon dioxide. Obtain biogas-related m...
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