Method for separating and purifying astaxanthin from antarctic krill shells

A technology for separation and purification of Antarctic krill, applied in chemical instruments and methods, organic chemistry, organic dyes, etc., can solve problems such as high production costs, protein waste, and industrial production restrictions

Inactive Publication Date: 2013-09-04
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The large-scale production of algae and yeast requires high-yield strains, strict environmental conditions and efficient wall-breaking measures, and the production cost is relatively high; while crustaceans are less developed and utilized due to the low content of astaxanthin
Moreover, whole Antarctic krill shrimps are mostly used as raw materials, which easily causes waste of protein during the extraction and purification process; at the same time, because most of the purification methods are high-efficiency liquid phases, industrial production is limited

Method used

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  • Method for separating and purifying astaxanthin from antarctic krill shells

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Take 100g of Antarctic krill shells, the moisture content is about 78.2%. Adjust the pH to 8.2, add Alcalase enzyme at an enzyme-to-substrate ratio of 3000U / g, and shake in a dark water bath at 55°C for 2.5h; the enzymolysis solution is filtered through gauze to obtain 74.2g of shrimp shell residue and filtrate, and the filtrate is centrifuged (4°C, 6000rap / min, 20min), to obtain 16.5g of enzymatic hydrolysis supernatant and pigment precipitate. Add acetone to the shrimp shell residue at a material-to-liquid ratio (w:v) of 1:5, shake in a water bath at 40°C in the dark for 3.5 hours, and filter to obtain the extract; Add ethanol, shake in a dark water bath at 40°C for 2.0 h, and filter with suction to obtain an extract. Combine the extracts of the shrimp shell residue and the pigment precipitation, concentrate at 40°C to remove the solvent, and obtain 0.70 g of crude pigment oil. Then dilute the crude pigment oil with absolute ethanol to a concentration of 0.1 g / mL, ...

Embodiment 2

[0026] Take 100g of Antarctic krill shells with a moisture content of 75.3%. Adjust the pH to 8.5, add Alcalase enzyme at an enzyme-to-substrate ratio of 3000U / g, and shake in a dark water bath at 55°C for 3.0h; the enzymolysis solution is filtered through gauze to obtain 72.6g of shrimp shell residue and filtrate, and the filtrate is centrifuged (4°C, 7000rap / min, 20min), to obtain 20.8g of enzymatic hydrolysis supernatant and pigment precipitate. Add acetone to the shrimp shell residue at a material-to-liquid ratio (w:v) of 1:4, shake in a water bath at 40°C in the dark for 4.0 hours, and filter to obtain the extract; Add ethanol, shake in a dark water bath at 40°C for 1.5 hours, and filter with suction to obtain the extract. Combine the extracts of shrimp shell residues and pigmentation, and concentrate at 40°C to remove the solvent to obtain 0.75 g of crude pigment oil. Then dilute the crude pigment oil with absolute ethanol to a concentration of 0.1 g / mL, add 1 / 5 volum...

Embodiment 3

[0029] Take 100g of Antarctic krill shells with a moisture content of 79%. Adjust the pH to 8.0-8.5, add Alcalase enzyme at an enzyme-to-substrate ratio of 3000U / g, and shake in a dark water bath at 55°C for 3.5h; the enzymolysis solution is filtered through gauze to obtain 70.5g of shrimp shell residue and filtrate, and the filtrate is centrifuged (4°C, 8000rap / min, 20min), to obtain 18.2g of enzymatic hydrolysis supernatant and pigment precipitate. Add acetone to the shrimp shell residue with a material-liquid ratio (w:v) of 1:6, shake in a water bath at 40°C for 3.0 hours, and filter to obtain the extract; Add ethanol, shake in a dark water bath at 40°C for 2.5 hours, and filter with suction to obtain the extract. Combine the extracts of the shrimp shell residue and the pigment precipitation, concentrate at 40°C to remove the solvent, and obtain 0.72 g of crude pigment oil. Then dilute the crude pigment oil with absolute ethanol to a concentration of 0.1 g / mL, add 1 / 5 vol...

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Abstract

The invention discloses a method for separating and purifying astaxanthin from antarctic krill shells. The method comprises the following steps of carrying out enzymolysis treatment on antarctic krill shells serving as raw materials through alcalase so as to obtain enzyme-hydrolyzed protein liquid, krill shell residues and pigment precipitates, extracting pigments from the krill shell residues and the pigment precipitates by respectively using acetone and ethanol, combining extracts, saponifying the combined extracts by using NaOH-ethanol so as to obtain saponified liquid, adsorbing free astaxanthin in the saponified liquid by using AB-8 macroporous resin, carrying out further separation and purification on the adsorbed free astaxanthin through a silica gel column, and carrying out freeze-drying so as to obtain high-purity astaxanthin powder. According to the method, the krill shells are processed by using the alcalase, so that the release of the astaxanthin is facilitated, and meanwhile, the protein is protected; and a method that macroporous adsorption resin and silica gel column chromatography are combined is adopted, so that the realization of large-batch production is facilitated.

Description

technical field [0001] The invention relates to a method for producing biological products from shrimp shells, in particular to a method for separating and purifying astaxanthin from Antarctic krill shells. Background technique [0002] In recent years, with the increasingly serious problem of resources, the development and utilization of pelagic resources has become a subject of research by various countries. It is estimated that the biomass of Antarctic krill is 379 million tons, almost all over the Antarctic sea area, and the resources are extremely rich. Countries pay close attention to the biomass and living habits of Antarctic krill in various sea areas, and the fishing volume is increasing year by year. Antarctic krill has high development and application value. In addition to being rich in protein, lipids and minerals, it also contains a variety of active substances, such as protein-digesting enzymes, astaxanthin and chitin. At present, domestic and foreign studies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C09B61/00
Inventor 姜启兴宋素梅王海鸥于沛沛夏文水许艳顺
Owner JIANGNAN UNIV
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