Method for producing reagent grade peptone

A production method and technology for peptone, applied in the biological field, can solve problems such as unsatisfactory yield of peptone, and achieve the effects of wide range of use, improved quality, and enhanced enzymatic hydrolysis effect.

Active Publication Date: 2015-02-11
GUIZHOU XINHUA BIOTECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The yield of peptone produced by this scheme is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Material selection and soaking: Select the raw materials according to the following weight percentage ratio and put them into the high-pressure steam pot, mix the raw materials and add 2 times the volume of water to soak for 3 hours:

[0030] Animal fresh bones or bone dregs 48%; animal offal dregs 34%; bean dregs 18%;

[0031] (2) High-temperature and high-pressure cooking: introduce steam into the pot, cook for 4 hours, and the pressure is 0.2Mpa;

[0032] (3) Separation and extraction: After cooking, reduce the pressure in the pot and drain the soup, skim off the foam and slick oil, separate the oil from the soup, and keep the soup;

[0033] (4) Protease hydrolysis: lower the temperature of the soup to 44°C, adjust the pH of the soup to 7.5 with a pH-adjusting base, weigh 7% of the total weight of the raw material for protease, dissolve it with 2 times the volume of the soup, add Carry out the hydrolysis reaction in the soup, keep the enzymolysis at a constant t...

Embodiment 2

[0040] (1) Material selection and soaking: Select the raw materials according to the following weight percentage ratio and put them into the high-pressure steam pot, mix the raw materials and add 2 times the volume of water to soak for 2 hours:

[0041] Fresh animal bones or bone dregs 45%; animal viscera dregs 35%; bean dregs 15%;

[0042] (2) High-temperature and high-pressure cooking: introduce steam into the pot, cook for 4 hours, and the pressure is 0.2Mpa;

[0043] (3) Separation and extraction: After cooking, reduce the pressure in the pot and drain the soup, skim off the foam and slick oil, separate the oil from the soup, and keep the soup;

[0044] (4) Protease hydrolysis: lower the temperature of the soup to 43°C, adjust the pH of the soup to 7.0-8.0 with a pH-adjusting base, weigh 6% of the total weight of the raw material for protease, and dissolve it with 2 times the volume of the soup , added to the soup for hydrolysis reaction, kept at a constant temperature of...

Embodiment 3

[0051] (1) Material selection and soaking: Select the raw materials according to the following weight percentage ratio and put them into the high-pressure steam pot, mix the raw materials and add 2 times the volume of water to soak for 2 hours:

[0052] Fresh animal bones or bone dregs 46%; animal offal dregs 34%; bean dregs 20%;

[0053] (2) High-temperature and high-pressure cooking: introduce steam into the pot, cook for 4 hours, and the pressure is 0.2Mpa;

[0054] (3) Separation and extraction: After cooking, reduce the pressure in the pot and drain the soup, skim off the foam and slick oil, separate the oil from the soup, and keep the soup;

[0055] (4) Protease hydrolysis: lower the temperature of the soup to 43.5°C, adjust the pH of the soup to 7.0-8.0 with a pH-adjusting alkali, weigh 6.5% of the total weight of the raw material for protease, and dissolve it with 2 times the volume of the soup , added to the soup for hydrolysis reaction, kept at a constant temperatur...

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PUM

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Abstract

The invention discloses a method for producing reagent grade peptone. The method comprises the following steps: selecting materials and soaking, performing high-temperature high-pressure digesting, performing separation and extraction, performing protease hydrolysis, heating and inactivating again, neutralizing, separating and filtering, and continuously condensing and drying at low temperature. The process is reasonable, low in cost, high in yield and short in production period, and the peptone product prepared by the method is high in quality, wide in application range, reaches the reagent grade standard and can be widely applied to the fields of modern biomedical research, detection of various bacteria, genetic engineering, fermentation in bioengineering, edible fungus cultivation and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a production method of peptone. Background technique [0002] Peptone is a pale yellow powder obtained by hydrolyzing meat, casein or gelatin with acid or protease and drying. It has a special smell of meat. Peptone can also be formed after protein is decomposed by acid, alkali or protease. Peptone is rich in organic nitrogen compounds, and also contains some vitamins and sugars, which can provide nutrients such as C source, N source, and growth factors for microorganisms, so it can be used as the main raw material for microbial culture media. Peptone is widely used in the fields of antibiotics, pharmaceutical industry, fermentation industry, biochemical products and microbiological research. Peptone can also be used to treat digestive tract diseases. Different organisms require specific amino acids and peptides, so there are various peptones. Generally speaking, the proteins used...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 陈俊锦
Owner GUIZHOU XINHUA BIOTECH DEV CO LTD
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