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Streptomycin high-yield strain and screening method thereof

A technology for high-yield strains and screening methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problem of low production capacity of streptomycin strains

Active Publication Date: 2013-09-11
HEBEI SHENGXUE DACHENG PHARMA
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Problems solved by technology

[0003] The purpose of the present invention is to provide a high-yielding bacterial strain of streptomycin, and provide a screening method for the strain at the same time, in order to improve the output of streptomycin and solve the problem that the production capacity of streptomycin is low in the production process

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  • Streptomycin high-yield strain and screening method thereof

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Embodiment 1

[0022] Example 1: DC622 strain screening

[0023] 1. Culture medium preparation

[0024] (1) The formula and preparation method of slant medium and solid double-disk medium are the same, that is, the ingredients are: 130g of yeast extract, 100g of glucose, 7g of dipotassium hydrogen phosphate, 6g of magnesium sulfate, and 230g of agar; Adjust the pH value of the culture medium to 6.7±0.1 with 20% sodium hydroxide solution, sterilize it at 0.11±0.01MPa, 121℃±1℃ for 20 minutes, and cool to room temperature for use.

[0025] (2) Liquid medium, calculated according to mass volume ratio, its composition is: yeast extract 70.0g, glucose 80.0g, potassium dihydrogen phosphate 10.0g, magnesium sulfate 0.6g, add water to 1000ml; use 20% sodium hydroxide Adjust the pH value of the medium to 7.0±0.1 with the solution; sterilize at 0.11±0.01MPa, 121°C±1°C for 20 minutes, and cool to room temperature for use.

[0026] (3) Sodium vanadate-containing solid double-disk medium formula: a...

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Abstract

The invention discloses a streptomycin high-yield strain and simultaneously provides a screening and fermenting method of the strain. The streptomycin high-yield strain provided by the invention is named streptomyces griseus (Streptomycesgriseus) DC622, is preserved in the Common Microorganism Center of China Committee for Culture Collection of Microorganisms (CGMCC) on June 14, 2012 and has a preservation number of CGMCCNO.6219. The screening method disclosed by the invention mainly comprises the following steps of: (a) obtaining original strain spore liquid; (b) carrying out mutagenesis of spore suspension; (c) screening high-phosphorus strains; (d) screening the high-yield strain; and (e) storing the high-yield strain. The invention also discloses a screening method of the DC622 strain. The average fermentation titer of the DC622 strain screened by the method can be up to 14908 U / mL.

Description

technical field [0001] The invention relates to a bacterial strain for producing antibiotics and a screening method thereof, in particular to a streptomycin high-yielding bacterial strain and a preparation method thereof. Background technique [0002] Streptomycin is the first aminoglycoside antibiotic discovered by Waksman (Waksman S1A1) in 1944. It has a strong antibacterial effect on Mycobacterium tuberculosis and many Gram-negative bacteria (G-) such as Escherichia coli , Klebsiella pneumoniae, Enterobacter, Salmonella, and Brucella also have certain antibacterial effects. The strains currently used for streptomycin fermentation are mainly Streptomyces griseus, Streptomyces bikini and the like. Streptomyces griseus, its appearance color is gray as the name says, the spore peduncle is straight and short, the single colony grows plumply, in the shape of plum blossom or steamed bread, with a diameter of 3-4 mm, the substrate hyphae are transparent, and light brown pigment ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00C12R1/545
Inventor 贾晓乐刘丽芳
Owner HEBEI SHENGXUE DACHENG PHARMA
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