Novel use of porphyrin modified electrode

A technology for modifying electrodes and new uses, applied in the field of electrochemical analysis, can solve the problems of poor selectivity and reproducibility of ascorbic acid, dopamine and uric acid, which cannot be detected at the same time

Inactive Publication Date: 2013-09-18
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing electrodes cannot simultaneously detect ascorbic acid, dopamine and uric acid with poor selectivity and reproducibility, and provide a new application of a porphyrin-modified electrode

Method used

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  • Novel use of porphyrin modified electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Polishing treatment of glassy carbon electrode: the glassy carbon electrode is polished into a mirror surface with 0.3 μm and 0.05 μm aluminum oxide suspension in turn, and then ultrasonically cleaned with 95% ethanol and double distilled water in turn to obtain a polished surface. glassy carbon electrode. Determination of the charge transfer resistance value (Rct) of the bare electrode: After the treated glassy carbon electrode was blown dry with nitrogen gas with a purity of 99.99%, it was inserted into 5 mL containing 5 mM potassium ferricyanide probe molecule with a molar concentration of 0.1 M potassium chloride electrolyte solution, and using a three-electrode system with glassy carbon electrode as working electrode, platinum wire as counter electrode, and Ag / AgCl as reference electrode for AC impedance scanning, the obtained figure 2 curve a in

[0027] Rinse the polished glassy carbon electrode with twice distilled water, dry it with nitrogen gas with a purity...

Embodiment 2

[0029] The trans-TPP (NH 2 ) 2 The modified glassy carbon electrode was placed in 1.5mL of 1mM HCl aqueous solution, and then 3mL of 5mM NaNO was added dropwise. 2 aqueous solution, kept at 0°C, protected by nitrogen gas, reacted for 15 minutes, and carried out the second diazotization (during this process, NaNO 2 The dosage should be excessive, so that the trans-TPP(NH 2 ) 2 fully converted to TPP). Take out the glassy carbon electrode, rinse it with absolute ethanol, dry it with nitrogen with a purity of 99.99%, and soak it in 0.1-3 mg / mL ɑ-Al 2 o 3 After about 6 hours in the aqueous solution, rinse with absolute ethanol, twice distilled water, and blow dry with nitrogen with a purity of 99.99%, to obtain TPP / ɑ-Al 2 o 3 Modified glassy carbon electrodes. Insert 5 mL of 0.1 M potassium chloride electrolyte solution containing 5 mM potassium ferricyanide probe molecules, and use a modified glassy carbon electrode as the working electrode, platinum wire as the counter e...

Embodiment 3

[0033] Detection of ascorbic acid (AA), dopamine (DA) and uric acid (UA) at a porphyrin-modified electrode

[0034] The bare glassy carbon electrode and TPP / ɑ-Al 2 o 3 The modified glassy carbon electrode was rinsed with double distilled water, dried with nitrogen gas with a purity of 99.99%, and placed in a phosphate buffer solution (PH=7) of 0.1-0.6mM ascorbic acid (AA). The three-electrode system with platinum wire as the counter electrode and Ag / AgCl as the reference electrode was scanned for cyclic voltammetry. The potential window was -0.3-0.7V, and the scan rate was 100mV / s. Two different cyclic voltammograms were obtained for plotting. Compared, the result is as image 3 As shown, TPP / ɑ-Al 2 o 3 Modified glassy carbon electrode (curve a) and bare glassy carbon electrode (curve b), from image 3 TPP / ɑ-Al can be seen in the 0.1-0.6mM AA oxidation process 2 o 3 Compared with the bare electrode, the peak potential of the modified electrode has shifted by ΔP=220-230m...

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Abstract

The invention discloses a novel use of a porphyrin modified electrode. The porphyrin modified electrode is obtained by decorating porphyrin and alpha-Al2O3 onto a glassy carbon electrode, and the porphyrin modified electrode is used for simultaneously detecting ascorbic acid, dopamine and uric acid. Since a porphyrin ring forms a pi-pi conjugated structure with aromatic nucleus in the ascorbic acid, the dopamine and the uric acid, and pyridine N of the porphyrin ring can form a hydrogen bond with hydroxyl and amino in the ascorbic acid, the dopamine and the uric acid, so that the porphyrin modified electrode has excellent chemical catalytic effect on the three micromolecule substances, the micromolecule substances can be rapidly and accurately separated in the cyclic voltammetry, and the porphyrin modified electrode can be used for simultaneously detecting the ascorbic acid, the dopamine and the uric acid and can detect simply, sensitively and rapidly.

Description

technical field [0001] The invention relates to the field of electrochemical analysis, in particular to a new application of a porphyrin-modified electrode. Background technique [0002] Ascorbic acid (AA), dopamine (DA) and uric acid (UA) are small molecular substances in the living body. It is of great significance to analyze the content of these three substances in the body. Among various analysis methods, the electrochemical method is simple because of its process. , fast response time and high sensitivity have received the most attention. Based on the fact that ascorbic acid, dopamine, and uric acid all contain active groups that are easily oxidized, however, the redox of these substances on the bare electrode is always irreversible and requires a high overpotential; Therefore, the selectivity and reproducibility are very poor, and it is difficult to separate their electrochemical signals, and it is impossible to realize the simultaneous detection of the three. Theref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 卢小泉冯彦俊李锦书陕多亮胡琛娴艾瑞霞连环
Owner NORTHWEST NORMAL UNIVERSITY
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