Biosensor construction and detection method based on molecular motor, magnetic enrichment and double-probe hybridization

A molecular motor, biotin technology, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc., can solve problems such as inability to detect multiple times, unfavorable comparisons, and limited detection sensitivity.
CN103308690AInactive Publication Date: 2013-09-18INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
Publication Date
2013-09-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a detection method of biomacromolecule, and in particular relates to a method for detecting the biomacromolecule by utilizing the signal amplification characteristic of the ATP molecular motor in terms of time and space and high temperature resistance thereof, high specificity of double-antibody hybridization and double-probe hybridization, high flexibility and fastness of magnetic enrichment and magnetic separation, and the high flexibility of the chemiluminiscence detection technology, and reduction of the non-specific hybridization by the single-stranded nuclease degradation and mismatched double-strand. A novel biosensor construction and detection method is capable of separating the signal molecule from the reaction system, storing the signal molecule to measure for multiple times and facilitating the comparison of the samples in different batches, and according to the method, the super-flexibility, fastness, high specificity and avoidance of amplification can be realized.
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Description

technical field

[0001] The invention relates to a detection method of biological macromolecules. In particular, it relates to a method for detecting biomacromolecules with high sensitivity using ATP molecular motors, dual-probe hybridization, magnetic enrichment and separation, and chemiluminescent detection techniques. Background technique

[0002] A biosensor is a device that uses a combination of biological elements and physical and chemical detection elements to detect an analyte. The double-antibody sandwich method is one of the traditional biomacromolecule detection methods. Its basic principle is to use a pair of specific probes (antibodies or nucleic acids) that can recognize different epitopes of the same molecule to detect through different signal output systems. For the detection of protein macromolecules, the commonly used method is enzyme-linked immunosorbent assay based on antigen-antibody binding. For nucleic acid macromolecules (including DNA, RNA, microRNA,...

Claims

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