Enzyme feed additive

A technology for feed additives and culture strains, applied in enzyme feed additives, enzymes in the field of feed applications, can solve the problems of cellulase production and application limitations, high production costs, poor enzyme stability, etc., to achieve the improvement of cultured food Safe, beneficial to environmental protection, and the effect of enhancing disease resistance

Inactive Publication Date: 2013-09-25
张惠蓉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the physical and chemical properties and mechanical properties of bacterial cellulase are superior to those of plant cellulase, the production and application of cellulase are hindered by the low enzyme production, low activity, poor enzyme stability and high production cost of bacteria at home and abroad. heavily restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Cultivation of Chaetomyces thermophilic-grade slant:

[0019] The thermophilic Chaetomium was inoculated on the primary slant medium by conventional methods, and cultivated at 30° C. for 24 hours to obtain a single colony of the thermophilic Chaetomium; the medium was a mixture prepared in the following ratio ( g / L): beef peptone: 5.0, yeast extract: 15.0, glucose: 3.0, MgSO 4 ·7H 2 O: 0.5, K 2 HPO 4 : 1.0, MnSO 4 : 20ppm, CaCO 3 : 5.0, agar 20, pH 7.0;

[0020] 2. Secondary liquid culture of Chaetomium thermophiles

[0021] Inoculate the single colony of the first-level thermophilic Chaetomium cultured in step 1 into the second-level culture medium by a conventional method, with a liquid volume of 20ml / 500ml, and cultivate it for 25 hours at 35°C and 180rpm / min to obtain a second-level thermophile Chaetomium seed liquid; the medium is a mixed solution (g / L) prepared in the following proportions: soybean peptone: 5.0, yeast extract: 5.0, glucose: 3.0, MgSO 4 ...

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PUM

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Abstract

Belonging to the field of bioengineering, the invention relates to application of enzymes in feed, in particular to an enzyme feed additive. The invention solves the disadvantage of a low utilization rate in traditional poultry feed. Employment of the enzyme feed additive provided in the invention can improve the nutritional value of original feed, prevent diseases and reduce feed consumption. The additive is prepared by using beef peptone, yeast extract, glucose and the like as a carbon source to perform fermentation, and then conducting drying. With no toxic or side effect, no drug resistance, and no residue, the enzyme feed additive can tolerate machining and the intestinal environment, and is shelf-stable.

Description

technical field [0001] The invention relates to the application of enzymes in feed, belonging to the field of bioengineering, in particular to an enzyme feed additive. Background technique [0002] The quail (Coturnix coturnix) is a smaller species of pheasant. There are many advantages to raising quail at home. It can get better economic benefits than raising quail, and the quail economy matures quickly, and quail farmers can start to make profits in less than two months. Its turnover rate is unmatched by any poultry. Although the eggshell of quail eggs is thin, the structure is tight. The inner shell membrane is tougher than quail eggs, and there is a layer of yellow oil on the shell surface. It will not be bad when stored for 50-60 days in hot summer, and it is more durable than quail eggs. [0003] With the development of the economy, people's living standards are also constantly improving, and people's demand for poultry is increasing, and the intensive and mechanized...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165C12N1/14C12R1/645
Inventor 张惠蓉
Owner 张惠蓉
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