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Technology of preparing acid-soluble bovine tendo calcaneus collagen by freeze thawing-ultrasonic method

An ultrasonic method, the technology of bovine Achilles tendon, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problem of low extraction rate and achieve the effect of improving extraction rate, excellent performance, and fewer types

Inactive Publication Date: 2013-10-02
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soak the Achilles tendon with an organic acid such as acetic acid to extract the collagen, the extraction rate is very low, so the acid method is often used in combination with the enzyme method to extract collagen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment one 1. Pretreatment: buy fresh beef Achilles tendon, and use a stainless steel knife to remove the fascia and fat on the tendon. Cut the beef Achilles tendon with fascia and fat removed into small pieces about 1 cm long. Soak in normal saline for 18 hours, replace the normal saline and soak for another 2 hours. Take out the small pieces of Achilles tendon and rinse them with pure water until the rinse solution is relatively clear. The small pieces of beef Achilles tendon after washing were filtered with a stainless steel filter, wrapped with plastic wrap, and placed in a refrigerator at -20°C for 18 hours. Take out the small pieces of beef Achilles tendon after freezing, and cut it into thin slices with a thickness less than 0.5mm. Spread the beef Achilles tendon slices on the plastic wrap, place them on the ultra-clean table for ventilation, and dry them in the air. After three days, collect the dried tendon sheets for use;

[0014] 2. Swelling: Add 300...

Embodiment 2

[0018] Embodiment two 1. Pretreatment: buy fresh beef Achilles tendon, and use a stainless steel knife to remove the fascia and fat on the tendon. Cut the beef Achilles tendon with fascia and fat removed into small pieces about 1 cm long. Soak in saline for 24h. Take out the small pieces of Achilles tendon and rinse them with pure water until the rinse solution is relatively clear. The small pieces of beef Achilles tendon after washing were filtered with a stainless steel filter, wrapped with plastic wrap, and placed in a refrigerator at -20°C for 12 hours. Take out the small pieces of beef Achilles tendon after freezing, and cut it into thin slices with a thickness less than 0.5mm. Spread the beef Achilles tendon slices on the plastic wrap, place them on the ultra-clean table for ventilation, and dry them in the air. After three days, collect the dried tendon sheets for use;

[0019] 2. Swelling: Add 400mL of 30% acetic acid solution and 3g of bovine Achilles tendon sli...

Embodiment 3

[0023] Embodiment three 1. Pretreatment: buy fresh beef Achilles tendon, and use a stainless steel knife to remove the fascia and fat on the tendon. Cut the beef Achilles tendon with fascia and fat removed into small pieces about 1 cm long. Soak in normal saline for 24 hours, replace the normal saline and soak for another 2 hours. Take out the small pieces of Achilles tendon and rinse them with pure water until the rinse solution is relatively clear. The small pieces of beef Achilles tendon after washing were filtered with a stainless steel filter, wrapped with plastic wrap, and placed in a refrigerator at -20°C for 18 hours. Take out the small pieces of beef Achilles tendon after freezing, and cut it into thin slices with a thickness less than 0.5mm. Spread the beef Achilles tendon slices on the plastic wrap, place them on the ultra-clean table for ventilation, and dry them in the air. After three days, collect the dried tendon sheets for use;

[0024] 2. Swelling: Add ...

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Abstract

The invention relates to a technology of preparing acid-soluble bovine tendo calcaneus collagen by a freeze thawing-ultrasonic method. The technology comprises the specific steps of: (1) bovine tendo calcaneus pretreatment: removing impurities from fresh bovine tendo calcaneus, soaking with normal saline, washing with pure water, freezing, slicing and airing, (2) swelling: putting and soaking aired bovine tendo calcaneus slices in an acetic acid solution for swelling, (3) beating: adding pure water into a swelled bovine tendo calcaneus suspension, performing mechanical beating to obtain crude slurry, (4) freeze thawing-ultrasonic: freezing the beaten bovine tendo calcaneus crude slurry, then performing ultrasonic treatment, repeating the freeze thawing-ultrasonic process to obtain a crude collagen extract, and (5) purification: performing filtering, centrifuging, salt precipitation, dialysis and freeze drying on the crude collagen extract to obtain the collagen product. The extraction method of the bovine tendo calcaneus collagen is simple and convenient and low in cost, the extraction rate of the collagen is increased without using enzyme, the purity of the obtained collagen is high, and a natural structure of the collagen is reserved. The prepared collagen can be applied to industries of medicine, cosmetics and the like.

Description

technical field [0001] The invention relates to a process for preparing acid-soluble bovine Achilles tendon collagen by a freeze-thaw-ultrasonic method, in particular to a process for extracting natural collagen by a non-enzymatic method. The invention belongs to the technical field of collagen extraction in organisms. Background technique [0002] The skin, bones, and tendons of vertebrates contain a large amount of collagen. There are various types, but type I collagen is the most abundant and widely used. The source of bovine Achilles tendon is rich, and the content of type I collagen is very high, which is a good source of raw material for extracting type I collagen. Type I collagen molecules are composed of three peptide chains coiled to form a unique triple helical structure, each chain contains a large number of repeated Gly-X-Y amino acid sequences, and the content of proline and hydroxyproline is relatively high. Collagen molecules contain a large number of hydro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/14
Inventor 穆畅道周颖郭佶慜李德富林炜
Owner SICHUAN UNIV
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