Fluorescent microsphere suitable for rapid immunology detection and preparation method thereof

Abstract

The invention relates to a fluorescent microsphere in the field of biology engineering technology, and especially relates to a fluorescent microsphere suitable for rapid immunology detection and a preparation method thereof. In the first step, polymer particles comprising fluorescence molecules, and an olefin monomer having functional groups that have modification functions, are used as raw materials for the inner core and the outer shell respectively; and polymer microspheres, external surfaces of which have functional groups having modification functions, are prepared through a microemulsion polymerization method. In the second step, according to a ratio of 1% (w / v) to 5% (w / v), the polymer microspheres prepared in the first step are slowly injected into an acetone or dichloromethane solution comprising fluorescence molecules; the polymer microspheres are swelled and the fluorescence molecules penetrate into the polymer microspheres; and then the fluorescent microspheres are prepared after steps of centrifuging to remove supernate, cleaning, adding water and suspending. The preparation method is simple. The product can be used for preparing precise and quantified immune chromatography test strips, and is suitable for detection technology fields of medical diagnosis, biological detection, environmental detection, food safety monitoring, and detection of different-species.

Description

technical field [0001] The invention relates to a fluorescent microsphere in the technical field of bioengineering and a preparation method thereof, in particular to a fluorescent microsphere suitable for immunological rapid detection and a preparation method thereof. Background technique [0002] In biological and medical testing, immunoassays are often used, including radioimmunoassays, enzyme-linked immunoassays, and immunochromatography. Radioimmunoassay and enzyme-linked immunoassay require expensive equipment, professional operators and complicated operation steps, and it is difficult to obtain test results quickly. Immunochromatography is often used for rapid qualitative or semi-quantitative detection because of its simple operation, rapidity and low cost. [0003] Immunochromatography technology is a unique immunoassay method that appeared in the early 1980s. It usually uses strip-shaped fiber chromatography materials as the solid phase, and the sample solution swim...

AI Technical Summary

Problems solved by technology

The disadvantage of emulsion polymerization is that the emulsifier added in the polymerization process affects the performance of the product

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