Bacterium for degrading herbicide chlorimuron-ethyl and application of bacterium

A technology for chlorflusulfuron and a bacterial agent, which is applied in the field of bacteria for degrading the herbicide chlorflusulfuron, can solve the problems of aging of branches and stem bases, loss of agricultural production, delayed maturity and the like

Active Publication Date: 2013-10-02
优农道(北京)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The extensive use of chemical herbicides has greatly improved the efficiency of agricultural production, but at the same time it has also brought about great production and environmental problems. For example, the phytotoxicity of long-residual herbicides to subsequent crops is becoming more and more serious, which affects the adjustment of agricultural planting industry structure and brings great impact on agricultural production. severe loss of production
Chlorimuron-methyl has the advantages of less dosage, high activity, and good weeding effect, and is deeply loved by farmers. However, chlorimuron-methyl is a long-acting herbicide with a long resi...

Method used

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  • Bacterium for degrading herbicide chlorimuron-ethyl and application of bacterium
  • Bacterium for degrading herbicide chlorimuron-ethyl and application of bacterium
  • Bacterium for degrading herbicide chlorimuron-ethyl and application of bacterium

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1. Isolation and Identification of Enterobacter sp. LMHL1716CGMCC No.7772

[0039] 1. Isolation of chlorimuron-methyl-degrading bacteria LMHL1716

[0040] Add 10 g of soil samples (collected from farmland polluted by the herbicide chlorimuron-methyl in Shaanxi Province, China) to 100 mL of enrichment culture solution containing 20 mg / L of chlorimuron-methyl, and culture for 7 days at 28°C and 180 r / min with shaking to enrich chlorine rimsulfuron-methyl-degrading bacteria. After the first round of enrichment, 10 mL of the enrichment solution was transferred to 100 mL of the enrichment medium containing 40 mg / L of chlorimuron-methyl, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture for 5 times, the concentrations of chlorimuron-methyl were 20, 40, 60, 80 and 100 mg / L in turn.

[0041] The screening of degrading bacteria adopts the plate transparent circle method. Add chlorimuron-methyl to inorga...

Embodiment 2

[0063] Example 2, Quantitative Determination of Enterobacter sp. LMHL1716CGMCC No.7772 Degradation Chlorsulfuron-methyl Ability

[0064] 1. Preparation of standard curve for the determination of chlorimuron-methyl

[0065] Use methanol to prepare chlorimuron-methyl standard substance (purchased from Fluka Company) into a series of standard solutions with concentrations of 10, 20, 40, 60, 80, and 100 mg / L, and use high performance liquid chromatography (HPLC) to determine different concentrations of chlorimuron-methyl The peak area of ​​the standard was repeated three times. With the concentration of chlorimuron-methyl as the abscissa and the peak area as the ordinate, draw the chlorimuron-methyl standard curve.

[0066] The detection conditions are as follows:

[0067] Detection system: Agilent1100Series. Chromatographic column: C18Diamosil TM Reversed-phase column, 250mm×4.6mm, particle size 5μm. Chromatographic conditions: mobile phase: acetonitrile: methanol: water: gl...

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Abstract

The invention discloses a bacterium for degrading a herbicide chlorimuron-ethyl and application of the bacterium. The bacterium is enteriobacter sp. LMHL1716, which is preserved in China General Microbiological Culture Collection Center by a preservation number of CGMCC No.7772. The enterobacter sp. LMHL1716 CGMCC No.7772 reaches a degrading rate of 70.44% on 45mg/L of chlorimuron-ethyl in an organic salt culture medium within 7d, showing that the strain can effectively degrade the chlorimuron-ethyl; the bacterium has a wide application prospect in an aspect of repairing chlorimuron-ethyl pollution of soil.

Description

technical field [0001] The invention relates to a bacterium for degrading the herbicide chlorimuron-methyl and its application. Background technique [0002] Chlorimuron-methyl, English common name chlorimuron-ethyl, chemical name 2-(4-chloro-6-methoxypyrimidin-2-ylcarbamoylsulfamoyl) methyl benzoate, structural formula as shown in formula 1: [0003] [0004] Formula 1. [0005] Chlorimuron-methyl is an ultra-efficient sulfonylurea herbicide, and its target is ALS (acetolactate synthase) in plants, which inhibits the biosynthesis of branched-chain amino acids valine and isoleucine, resulting in the substrate α- The accumulation of butanone hinders the DNA synthesis in interphase of cell division, stops mitosis, and the cells cannot grow normally. Chlorimuron-methyl can be absorbed by the roots, stems, and leaves of plants, and conducts up and down in the plant body. It is a selective pre-emergence and post-emergence herbicide. It is mainly used in farmland such as soyb...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02B09C1/10C12R1/01A62D101/04A62D101/28
Inventor 孙建光杨汉高淼孙燕华
Owner 优农道(北京)科技有限公司
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