Preparation method of fibroin microsphere

A technology of silk fibroin and microspheres, applied in animal feed, animal husbandry, applications, etc., can solve the problems of different release mechanisms and time, and achieve the effects of slow and uniform degradation, excellent biocompatibility, and high embedding efficiency

Inactive Publication Date: 2013-10-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Microspheres with different preparation methods have different effects when embedding different drugs, and the release mechanism and time are also different. The design of the microsphere preparation method needs to be based on the characteristics of the drug. At present, there is no high embedding efficiency. Degradation and slow and uniform release, excellent biocompatibility, suitable for the preparation of silk fibroin microspheres for embedding and slow-release nanoparticle drugs, polypeptide drugs and enzyme drugs

Method used

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  • Preparation method of fibroin microsphere
  • Preparation method of fibroin microsphere

Examples

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Effect test

Embodiment 1

[0019] Prepared in the present embodiment are silk fibroin microspheres of slow-release nano-silver, and the steps of the preparation method of the silk fibroin microspheres are as follows: 1000 grams of silkworm cocoon shells are mixed with 0.5wt% Na 2 CO 3 The aqueous solution degumming is performed twice, 30 minutes each time, the degumming temperature is 100°C, and the bath ratio is 1:100. After degumming, it is fully washed with water and dried at 60°C to obtain silk fibers. with CaCl 2 Aqueous solution dissolves silk fiber, silk fiber: CaCl 2 2H 2 O: water=1g:25g:25ml, the dissolution temperature is 100°C, and the dissolution time is 5min. The solution obtained after dissolving is filtered and injected into a cellulose dialysis membrane for dialysis for 3 days. The molecular weight cut-off of the cellulose dialysis membrane used is 8000-14000. Concentrate the silk fibroin solution to 4wt%, add 0.3wt% nano-silver, dropwise add 5% diglycidyl ether, and mix slowly and ...

Embodiment 2

[0023] Prepared in the present embodiment is the silk fibroin microsphere of slow-release growth factor, and the steps of the preparation method of this silk fibroin microsphere are as follows: 1000 grams of silkworm cocoon shells are mixed with 0.5wt% Na 2 CO 3 The aqueous solution degumming is performed twice, 30 minutes each time, the degumming temperature is 100°C, and the bath ratio is 1:100. After degumming, it is fully washed with water and dried at 60°C to obtain silk fibers. with CaCl 2 Aqueous solution dissolves silk fiber, silk fiber: CaCl 2 2H 2 O: water=1g:25g:25ml, the dissolution temperature is 100°C, and the dissolution time is 5min. The solution obtained after dissolving is filtered, poured into a cellulose dialysis membrane for dialysis for 3 days, and the molecular weight cut-off of the cellulose dialysis membrane used is 8000-14000. The silk fibroin solution was concentrated to 4 wt%. Cool the sterilized silk fibroin solution to about 0°C, add 0.5wt% ...

Embodiment 3

[0026] Prepared in the present embodiment are silk fibroin microspheres of slow-release antimicrobial peptides, and the steps of the preparation method of the silk fibroin microspheres are as follows: 500 grams of silkworm cocoon shells are washed with 0.5wt% Na 2 CO 3The aqueous solution degumming is performed twice, 30 minutes each time, the degumming temperature is 100°C, and the bath ratio is 1:100. After degumming, it is fully washed with water and dried at 60°C to obtain silk fibers. Dissolve silk fiber with calcium chloride, water and ethanol solution with a molar ratio of 1:8:2, the dissolution temperature is 70°C, the bath ratio is 1:25, and the time is 10 minutes. The solution obtained after dissolving is filtered and poured into a cellulose dialysis membrane for dialysis for 3 days. The molecular weight cut-off of the cellulose dialysis membrane used is 8000-14000. Concentrate the silk fibroin solution to 5wt%, add 1wt% antibacterial peptide, add 5wt% ethanol drop...

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Abstract

The invention relates to a preparation method of a fibroin microsphere, and the fibroin microsphere can be taken as a carrier of medicaments for slow release. At present, there is not a preparation method of a demand-satisfying fibroin microsphere; and wherein the demands are that the fibroin microsphere has high embedding efficiency, and the medicament can be released slowly and uniformly along with the degradation of the prepared fibroin microsphere. The preparation method comprises the following steps: adding the medicament needing to embed and release into a regenerated fibroin solution with a concentration of 3-10 wt%, then dropwise adding a coagulating agent with a concentration of 2-10 wt%, and slowly stirring at a rotary speed of 80-120 r / min to obtain a mixed solution; spraying the mixed solution into a space with a temperature of -20 DEG C to -80 DEG C and coagulating, collecting icy particles, continually preserving the icy particles in a space with a temperature of -20 DEG C to -80 DEG C for more than 2 hours, then unfreezing the icy particles at 0 DEGC to room temperature, washing and removing the coagulating agent at 0 DEG C to room temperature, collecting the fibroin microsphere by centrifuging or filtering. The fibroin microsphere prepared by the method is high in embedding efficiency and excellent in biological compatibility, and the medicament is released slowly and uniformly along with the degradation of the fibroin microsphere.

Description

technical field [0001] The invention relates to a preparation method of silk fibroin microspheres, and the silk fibroin microspheres can be used as carriers for sustained release of medicines. Background technique [0002] Silk fibroin is composed of 18 kinds of amino acids. Materials made of silk fibroin have excellent biocompatibility and can be slowly degraded in vivo. Therefore, the application of silk fibroin microspheres in drug sustained-release carriers has attracted extensive attention. In the preparation of silk fibroin materials in the form of microspheres, there are currently emulsification (Yaowalak Srisuwanl), coagulation precipitation (Joydip Kundu), spray drying (Joo-Hong Yeo), spray freezing-freeze drying-crosslinking (Esther wenk) and other methods. . For example, the publication date is August 20, 2008, and the Chinese patent publication number CN101244277 discloses a preparation method of silk fibroin drug-loaded microspheres. Assembly technology, after...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K9/16A61L15/32A61L15/44A23K1/16
Inventor 吴豪翔王彦胡豆豆闵思佳
Owner ZHEJIANG UNIV
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