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Method for extracting high pure glabridin from glycyrrhiza glabra

A technology of licorice glabra and glabridin, which is applied in the direction of organic chemistry, can solve the problems that the purity of glabridin is not high enough, affects the purity of glabridin, and its content is low, and achieves low cost, high purity and short process flow Effect

Active Publication Date: 2013-10-23
天津尚美化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the content of glabridin in glabridin is extremely low, and separation and purification itself is a technical difficulty; and there are many impurities with very similar properties to glabridin, which affect the purity of glabridin, resulting in glabridin in the final product. Purity is not high enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting high-purity glabridin from Glycyrrhizae glabra, comprising the steps of:

[0021] (1) Add the mixed solution to the dry powder of Glycyrrhizae glabra at a ratio of 1:8 by mass, and extract it ultrasonically at 30°C, 50Hz, and 100w for 20min; the mixed solution is acetic acid with a volume ratio of 3:3:4 A mixture of ethyl ester, acetone and absolute ethanol;

[0022] (2) suction filtration, the filtrate reclaims the mixed solution, and the solid is freeze-dried to a fluffy state, and the solid in the fluffy state is dissolved in an appropriate amount of ethanol solution with a volume ratio of 70% (the solid can be completely dissolved in the ethanol solution as the criterion). Centrifuge at 10°C at 3000 rpm for 15 minutes to remove the precipitate to obtain a clear and transparent solution;

[0023] (3) The solution obtained in step (2) is added to the macroporous resin column, and the loading speed is 2BV / h. The macroporous resin in the macropo...

Embodiment 2

[0027] A method for extracting high-purity glabridin from Glycyrrhizae glabra, comprising the steps of:

[0028] (1) Add the mixed solution to the dry powder of Glycyrrhizae glabra in a mass ratio of 1:6, and extract it ultrasonically at 50°C, 30Hz, and 80w for 10min; the mixed solution is acetic acid with a volume ratio of 1:3:3 A mixture of ethyl ester, acetone and absolute ethanol;

[0029] (2) suction filtration, the filtrate reclaims the mixed solution, and the solid is freeze-dried to a fluffy state, and the solid in the fluffy state is dissolved in an appropriate amount of ethanol solution with a volume ratio of 50% (the solid can be completely dissolved in the ethanol solution as the criterion). Centrifuge at 5000 rpm at 4°C for 5 minutes to remove the precipitate to obtain a clear and transparent solution;

[0030] (3) The solution gained in step (2) is added to the macroporous resin column, and the loading speed is 1.5BV / h. The macroporous resin in the macroporous r...

Embodiment 3

[0034] A method for extracting high-purity glabridin from Glycyrrhizae glabra, comprising the steps of:

[0035] (1) Add the mixed solution to the dry powder of Glycyrrhizae glabra in a mass ratio of 1:7, and extract it ultrasonically at 40°C, 50Hz, and 120w for 25min; the mixed solution is acetic acid with a volume ratio of 2:2:4 A mixture of ethyl ester, acetone and absolute ethanol;

[0036] (2) suction filtration, the filtrate reclaims the mixed solution, and the solid is freeze-dried to a fluffy state, and the solid in the fluffy state is dissolved in an appropriate amount of ethanol solution with a volume ratio of 80% (the solid can be completely dissolved in the ethanol solution as the criterion). Centrifuge at 15°C at 4000 rpm for 10 minutes to remove the precipitate to obtain a clear and transparent solution;

[0037] (3) adding the solution obtained in step (2) to the macroporous resin column, the loading speed is 3BV / h, and the macroporous resin in the macroporous ...

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PUM

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Abstract

The invention discloses a method for extracting high pure glabridin from glycyrrhiza glabra. The method comprises the following steps of (1) adding a mixed solution in dry powder of glycyrrhiza glabra; extracting for 10-30 min under ultrasonic frequency of 30-80 Hz and ultrasonic power of 80-200 W at a temperature of 10-50 DEG C, wherein the mixed solution is prepared by ethyl acetate, acetone and absolute ethyl alcohol; (2) suction filtering, The method is simple in operational technology, short in process flow and low in cost. The mixed solution of ethyl acetate, acetone and absolute ethyl alcohol is used as an extraction solution, and a mixed resin is used for extraction and purification, so that the purity of the obtained glabridin is high.

Description

technical field [0001] The invention belongs to the field of extracting traditional Chinese medicines, and in particular relates to a method for extracting glabridin from glabra. technical background [0002] Glabridin is one of the main flavonoids in Glycyrrhizae glabra. It shows a strong anti-free radical oxidation effect in the cytochrome P450 / NADPH oxidation system, which can significantly inhibit the free radicals produced in the metabolic process in the body, so as to prevent oxidation-sensitive biological macromolecules (low-density lipoprotein LDL, DNA) and cell walls are oxidatively damaged by free radicals. Thereby, some pathological changes related to free radical oxidation can be prevented, such as atherosclerosis, cell aging and so on. In addition, glabridin still has a certain effect of lowering blood fat and blood pressure, and shows good prospects for medicinal development in the prevention and treatment of arteriosclerosis and cardiovascular diseases. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04
Inventor 郭毅群
Owner 天津尚美化妆品有限公司
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