Technology for preparation of succinic acid finished product by bioconversion of cassava raw material

A technology of biotransformation and succinic acid, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of high production costs, environmental pollution, low yield, etc., achieve low prices and reduce environmental pollution Effect

Active Publication Date: 2013-10-23
山东兰典生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, succinic acid is mainly prepared from petrochemical raw materials. Not only the production cost is too high, but also the pollution to the environment is relatively large, which limits its application.
[0004] There have also been some methods for preparing succinic acid by microbial fermentation, but the raw materials used are mostly food crops, the cost is high, and the yield is not high, and the effect is not very satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A kind of technology that prepares succinic acid finished product with cassava raw material biotransformation, adopts following steps:

[0062] A. Crushing of cassava pieces

[0063] Select high-quality cassava pieces, grind them with a sieve aperture Φ1.5mm grinder, and put them in the reaction kettle;

[0064] B. Liquefaction of cassava

[0065] Add water to the reaction kettle, the weight ratio of water to cassava pieces is 2:1, start stirring at a speed of 100r / min, turn on the power and heat to 80°C;

[0066] Add amylase with an amylase activity of 10000U / ml into the reaction kettle, the weight ratio of amylase to cassava pieces is 1:200, and keep the constant temperature at 80°C for 0.5h;

[0067] C. Saccharification of cassava

[0068] a. Turn off the reactor heater, turn on the cooling water, lower the cassava liquefaction solution to 55°C, add glucoamylase with an activity of 10000U / ml, the weight ratio of glucoamylase to cassava block is 1:40, and turn on t...

Embodiment 2

[0102] A kind of technology that prepares succinic acid finished product with biotransformation of cassava raw material, comprises the steps:

[0103] A. Crushing of cassava pieces

[0104] Select high-quality cassava pieces, grind them with a sieve aperture Φ1.5mm grinder, and put them in the reaction kettle;

[0105] B. Liquefaction of cassava

[0106] Add water into the reaction kettle, the weight ratio of water to cassava pieces is 2.5:1, start stirring at a speed of 120r / min, turn on the power and heat to 90°C;

[0107] Add amylase with an amylase activity of 10000U / ml into the reaction kettle, the weight ratio of amylase to cassava pieces is 1:170, and keep the temperature at 90°C for 1.0h;

[0108] C. Saccharification of cassava

[0109] a. Turn off the reactor heater, turn on the cooling water, lower the cassava liquefaction solution to 60°C, add glucoamylase with an activity of 10000U / ml, the weight ratio of glucoamylase to cassava block is 1:50, and turn on the re...

Embodiment 3

[0142] A kind of technology that prepares succinic acid finished product with biotransformation of cassava raw material, comprises the steps:

[0143] A. Crushing of cassava pieces

[0144] Select high-quality cassava pieces, grind them with a sieve aperture Φ1.5mm grinder, and put them in the reaction kettle;

[0145] B. Liquefaction of cassava

[0146] Add water into the reaction kettle, the weight ratio of water to cassava pieces is 3:1, start stirring at a speed of 150r / min, turn on the power and heat to 100°C;

[0147] Add amylase with amylase activity of 10000U / ml to the reaction kettle, the weight ratio of amylase to cassava pieces is 1:150, and keep the temperature at 100°C for 1.5h;

[0148] C. Saccharification of cassava

[0149] a. Turn off the reactor heater, turn on the cooling water, lower the cassava liquefaction solution to 65°C, add glucoamylase with an activity of 10000U / ml, the weight ratio of glucoamylase to cassava block is 1:60, and turn on the reactor...

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Abstract

Provided is a technology for preparation of succinic acid finished product by bioconversion of cassava raw material. The technology comprises the following steps: A, crushing of cassava pieces; B, liquification of cassava; C, saccharification of cassava; D, disinfection of fermentation tanks and material supplementing tanks; E, preparation of fermentation medium; F, disinfection of the fermentation medium; G, inoculated fermentation of the medium; H, filtration and decoloration of the fermentation solution; I, ion exchange of the fermentation liquor after decoloration; J, evaporation and concentration of succinic acid solution; K, crystallization of the succinic acid concentrated solution; and L, desiccation of the succinic acid crystals. The technology takes reasonable technology steps, pretreats non-grain crop cassava, selects specific strains, ferments the cassava raw material solution and obtains succinic acid. During the preparation process, the conversion rate of sugar to acid is extremely high and cassava is used fully.

Description

technical field [0001] The invention relates to the technical field of succinic acid preparation, in particular to a process for preparing succinic acid finished products through biotransformation of cassava raw materials. Background technique [0002] Succinic acid is a very important chemical raw material, mainly used in the preparation of pentaheterocyclic compounds such as succinic anhydride. Also used in the preparation of alkyd resins (alkyd resins produced from succinic acid have good flexibility, elasticity and water resistance), paints, dyes (diphenyl ester of succinic acid is an intermediate of dyes, and amino Anthraquinone reacts to produce anthraquinone dye), food flavoring agent (succinic acid can also be used as food sour agent for seasoning of wine, feed, candy, etc.), photographic materials, etc. In the pharmaceutical industry, it can be used to produce sulfa drugs, vitamin A, vitamin B and other antispasmodics, expectorants, diuretics and hemostatic drugs. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C07C55/10C07C51/42C12R1/19
Inventor 张乐红高金龙
Owner 山东兰典生物科技股份有限公司
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