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Setaria italica anti-rust gene coseparated molecular marker and detection method thereof

A technology of molecular markers and co-segregation, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of inability to assist breeding, and achieve planting cost saving, efficiency improvement, and accelerated breeding The effect of the process

Active Publication Date: 2013-11-06
GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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AI Technical Summary

Problems solved by technology

At present, there is only one research report on the rust-resistant gene AFLP marker published by our research group in 2010 on millet. The genetic distance between the marker and the rust-resistant gene is 7.4 cM, which cannot be used for assisted breeding in production.

Method used

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  • Setaria italica anti-rust gene coseparated molecular marker and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1. Obtaining a co-segregated molecular marker SNP+InDel / SLAF-75316 of a millet anti-rust gene

[0035] 1. Test materials

[0036] Using the source of high resistance to millet rust, Shilixiang, the high-susceptibility variety Yugu No. 1, and its hybrid F 1 , F 2 group.

[0037] 2. Identification of material rust resistance

[0038] Pair of parents Shilixiang and Yugu 1 and F 1 , F 2 Individual plants were identified for resistance inoculation. At the 5-leaf stage of millet, collect the virulent race A of millet rust 57 Monospora strain 93-5, prepare spore suspension (concentration of 1.0×10 5 individuals / mL) spray inoculated and moisturized for 20 h. After 12 days, when the susceptible plants were obviously onset, the onset of the parents and offspring were investigated, and the response type of each plant was determined. The survey standard of reaction type refers to the method of Liang Kegong et al., and is divided into 5 grades: grade 0 (immunity) - ...

Embodiment 2

[0081] Example 2: Marker genotype analysis of millet resistant materials using markers.

[0082] Millet susceptible materials include Kuanjiu, Xiaoxiangmi, Pin 103 (unified number is 27389), Pin 104 (27390), Pin 111 (27397), Pin 143 (27429), Pin 149 (27435), Pin 176 (27462) , Pin 206 (27609), Pin 207 (27610), Pin 208 (27611), Pin 211 (27614), Pin 212 (27615), Pin 213 (27616), one each, provided by the National Institute of Crop Science, Chinese Academy of Agricultural Sciences Preserved in the germplasm bank; Jingu rice was bred and preserved by the Millet Research Institute of Hebei Academy of Agricultural Sciences.

[0083] Millet disease-resistant materials include Ji Chuang No. 1, which is obtained by our research group by crossing with other millet varieties and breeding for many years, with ten lixiang as the male parent. It has the same stable anti-rust characteristics as ten lixiang.

[0084] We used this marker to conduct SNP+InDel / SLAF-75316 marker analysis on the a...

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Abstract

The invention relates to a setaria italica anti-rust gene coseparated molecular marker and a detection method thereof. The setaria italica anti-rust gene coseparated molecular marker is used for anti-rust resource utilization and anti-rust variety breeding. The setaria italica anti-rust gene coseparated molecular marker is SNP+InDel / SLAF-75316. The coseparated molecular marker SNP+InDel / SLAF-75316 is used for assistant selective breeding. Through combination with a field inoculation identification method, novel anti-rust variety selective breeding is promoted; anti-rust variety breeding efficiency is high; environmental pollution caused by pesticide-based rust prevention and treatment is reduced; a plantation cost is reduced; and obvious economic and ecological benefits are obtained.

Description

technical field [0001] The invention relates to a molecular marker for co-separation of anti-rust genes in millet and a detection method thereof, belongs to the field of molecular breeding, and is used for the utilization of anti-rust resources of millet and the selection and breeding of anti-rust varieties. Background technique [0002] Millet ( Setaria italica (L.) Beauv.) Drought-resistant, barren-resistant, food and grass, rich in nutrition, distributed in Asia, Europe and other places. The planting area and output of millet in China rank first in the world, and it is an important food crop in the north. [0003] Millet rust is the fungus millet rust ( Uromyces setariae-italicae Yoshino) is the main disease in millet production, generally reducing the yield by 10% to 30%. In the epidemic year, the plants are lodging and the grains are not harvested, which seriously affects the stable and high yield of millet. With the improvement of industrialization level and clim...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 白辉李志勇朱彦彬董志平全建章董立赵立强刘磊马继芳
Owner GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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