Fluorescence imaging system and application thereof

Abstract

The invention relates to the field of an optical technology, in particular to a short wave near infrared quantum dot imaging system, which comprises a laser light path, wherein the laser light path comprises a laser, a coupler, a single-mode optical fiber and a beam expanding module; a near infrared beam is emitted by the laser to pass through the coupler in the laser light path, is introduced into the single-mode optical fiber and then is irradiated to an objective table on which a sample is carried through the beam expanding module; and excited fluorescence of the sample passes through a filter set and an imaging objective and then reaches a detector. According to the short wave near infrared quantum dot imaging system, the near infrared laser can be used as a light source to irradiate into the body of a small animal, and quantum dots in the body are excited to emit fluorescence, so that deep tissues, organs and cells in the body of the living small animal can be quickly, efficiently and nondestructively observed in real time, and high-efficiency technical support is provided for research in the aspects such as tumor cells and stem cells in the future; and the appearance characteristics of other materials of the near infrared bands can also be observed.

Description

technical field

[0001] The invention relates to the field of optical technology, in particular to a fluorescence imaging system with an operating wavelength range in the near-infrared band and an application thereof. Background technique

[0002] At present, bioluminescence and fluorescence imaging are mainly used for in vivo imaging of small living animals. Bioluminescence is to use luciferase gene to mark cells or DNA, and in vivo fluorescence imaging technology mainly has three marking methods: fluorescent protein labeling, fluorescent dye labeling and quantum dot labeling. In comparison, quantum dots, as a new type of nano-fluorescent probe, have many advantages such as wide excitation spectrum, narrow fluorescence emission spectrum, adjustable fluorescence spectrum, high quantum yield, high photochemical stability and not easy to decompose.

[0003] Due to the different tissue penetration of different wavelengths, the absorption of near-infrared wavelengths by hemoglob...

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