Crocodile meat oral liquid and preparation method thereof
A technology for crocodile meat and oral liquid, which is applied to the field of crocodile meat oral liquid and its preparation, can solve problems such as the treatment of substances without bitter and fishy taste, and achieve the effects of good taste, high content and better taste.
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Embodiment 1
[0026] The screening of enzyme in the preparation method of embodiment 1 crocodile meat oral liquid
[0027] 1 Experimental materials
[0028] Main instruments: analytical balance, acidity meter, magnetic stirrer (Hangzhou Instrument Electric Co., Ltd.), electric heating constant temperature water bath (HH, SII-NI4B Beijing Jingke Huarui Co., Ltd.), DS-1 high-speed tissue masher (Shanghai Specimen Model factory), TDZ5-WS centrifuge (Changsha Xiangzhi Centrifuge Instrument Co., Ltd.), high performance liquid chromatography, UV-visible detector, data workstation, ultrasonic cleaner.
[0029] Reagents: 2 catties of fresh crocodile meat (Thai crocodile, Crocodylus siamensis Schneider, purchased from Guangzhou Crocodile Park), papain (enzyme activity 400U / mg, Guoao Biological Company sigma), flavor protease (enzyme activity 20U / mg, Guangzhou Hua Qisheng Biotechnology Co., Ltd. (Amresco), pepsin (10U / mg, sigma), compound protease (120U / mg, LOT#KY201202, Guangzhou Qiyun Biotechnology...
Embodiment 2
[0089] Inclusion condition screening in the preparation method of embodiment 2 crocodile meat oral liquid
[0090] 1 Experimental materials
[0091] Experimental reagent: β-cyclodextrin
[0092] Main instruments: electric heating constant temperature water bath, analytical balance
[0093] 2 Experimental methods
[0094] single factor experiment
[0095] 2.1 Effect of temperature on inclusion effect
[0096] Get the crocodile meat enzymolysis solution obtained by enzymatic hydrolysis under the best enzymolysis conditions of Example 1, utilize the sensory evaluation standard in Example 1 to evaluate its bitter fishy smell, its bitterness is 4.9 points, and fishy smell is 5.0 points, to the enzymolysis solution 2% (w / v) of β-cyclodextrin was added to the mixture, the inclusion time was fixed at 1h, and the influence of temperature on the effect of debittering and deodorizing was investigated.
[0097] Table 7. Effect of β-cyclodextrin inclusion temperature on the effect
...
Embodiment 3
[0106] Fermentation condition screening in the preparation method of embodiment 3 crocodile meat oral liquid
[0107]1 Experimental materials
[0108] Experimental reagent: yeast powder
[0109] Main instruments: electric heating constant temperature water bath, analytical balance
[0110] 2 Experimental methods
[0111] single factor experiment
[0112] 2.1 The influence of the dosage of yeast powder
[0113] Get the solution after inclusion obtained in Example 2, utilize the sensory evaluation standard in Example 1 to evaluate its bitter fishy smell, its bitter taste is 6.0 points, and fishy smell is 5.4 points, respectively add different A large amount of yeast powder was fermented at a constant temperature at 40°C for 1 hour. After the fermentation, the supernatant was separated by centrifugation, and the enzyme was inactivated in a boiling water bath for 5 minutes.
[0114] Table 9. The effect of the amount of yeast powder on the effect of debittering and deodorizing...
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