Method for determining total solanesol in tobaccos and tobacco products

A technology for tobacco products and total solanesol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable batch operation, consumption of organic solvents, low detection efficiency, etc., achieve less solvent consumption, reduce pollution, and accurately The effect of high precision and precision

Inactive Publication Date: 2013-11-20
TOBACCO RES INST CHIN AGRI SCI ACAD
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Problems solved by technology

Existing tobacco solanesol is generally determined by Soxhlet extraction with non-polar solvents (n-hexane, petroleum ether, etc.), the extract is saponified under heating and reflux in a water bath, and the saponified extract is obtained by liquid-liquid

Method used

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  • Method for determining total solanesol in tobaccos and tobacco products
  • Method for determining total solanesol in tobaccos and tobacco products
  • Method for determining total solanesol in tobaccos and tobacco products

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[0018] A method for measuring total solanesol in tobacco and tobacco products, the steps comprising:

[0019] 1. Simultaneous ultrasonic extraction and saponification of tobacco solanesol: take tobacco or tobacco products and grind it into tobacco powder, place it in a centrifugal glass tube, add extraction solvent and saponification agent respectively, and simultaneously extract and saponify tobacco solanesol by ultrasonic;

[0020] 2. Washing and separation of tobacco solanesol extract: the extract is cooled to room temperature, a certain volume of water is added, fully shaken, and centrifuged;

[0021] 3. Pipette 0.500mL of the upper layer solution, dilute the mobile phase to 5.000mL, and filter it with a 0.22um organic filter into a liquid chromatography ampoule for testing.

[0022] 4. Perform liquid chromatography on the obtained liquid to be tested.

[0023] Further, in the step 1, tobacco or tobacco products include various types of modulated tobacco leaves, green tob...

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Abstract

The invention discloses a method for determining total solanesol in tobaccos and tobacco products. The method comprises the following steps of: 1, carrying out simultaneous supersonic extraction and saponification on tobacco solanesol, specifically, taking the tobaccos or the tobacco products and grinding the tobaccos or the tobacco products into tobacco powder; placing the tobacco powder into a centrifugal glass tube and adding an extraction solvent and a saponification agent respectively; simultaneously carrying out the supersonic extraction and the saponification on the tobacco solanesol; 2, washing and separating a tobacco solanesol extracting solution, specifically, cooling the extracting solution to a room temperature and adding a certain volume of water; completely vibrating and centrifuging; 3, taking 0.500mL of an upper-layer solution and diluting a flowing phase until the volume is 5.000mL; filtering the solution to a liquid chromatogram ampere bottle by using a 0.22-micron organic filtering membrane to be detected; and 4, carrying out liquid chromatogram determination on the obtained solution to be detected. According to the method disclosed by the invention, the extraction, the saponification and the separation are all finished in the centrifugal tube; the method has the advantages of small solvent dosage, simplicity in operation, cost saving, pollution reduction and improvement of working efficiency; the method is suitable for analyzing samples in a large batch, and the accuracy and the precision of determined results are high.

Description

technical field [0001] The invention relates to a method for detecting tobacco active substances, in particular to a method for measuring total solanesol in tobacco and tobacco products. Background technique [0002] Tobacco is the plant with the highest content of solanesol. Solanesol has antibacterial, anti-inflammatory, cardiovascular disease and anti-ulcer effects. It is an irreplaceable intermediate in the synthesis of coenzyme Q10 and vitamin K2, and can be used to synthesize the anticancer synergist SDB . [0003] At present, the detection of tobacco solanesol generally adopts chromatographic analysis method. Due to the large molecular weight and high boiling point of solanesol, the determination of solanesol by gas chromatography requires complex derivatization treatment. Liquid chromatography is a commonly used method for solanesol. [0004] Solanesol exists in tobacco in two forms: free alcohol and compounded state (mainly acetate, palmitate, linoleate). Therefore...

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Application Information

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IPC IPC(8): G01N30/06
Inventor 杜咏梅张怀宝侯小东李丹丹付秋娟刘翠翠蒯雁
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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