Method for determining total solanesol in tobaccos and tobacco products

Abstract

The invention discloses a method for determining total solanesol in tobaccos and tobacco products. The method comprises the following steps of: 1, carrying out simultaneous supersonic extraction and saponification on tobacco solanesol, specifically, taking the tobaccos or the tobacco products and grinding the tobaccos or the tobacco products into tobacco powder; placing the tobacco powder into a centrifugal glass tube and adding an extraction solvent and a saponification agent respectively; simultaneously carrying out the supersonic extraction and the saponification on the tobacco solanesol; 2, washing and separating a tobacco solanesol extracting solution, specifically, cooling the extracting solution to a room temperature and adding a certain volume of water; completely vibrating and centrifuging; 3, taking 0.500mL of an upper-layer solution and diluting a flowing phase until the volume is 5.000mL; filtering the solution to a liquid chromatogram ampere bottle by using a 0.22-micron organic filtering membrane to be detected; and 4, carrying out liquid chromatogram determination on the obtained solution to be detected. According to the method disclosed by the invention, the extraction, the saponification and the separation are all finished in the centrifugal tube; the method has the advantages of small solvent dosage, simplicity in operation, cost saving, pollution reduction and improvement of working efficiency; the method is suitable for analyzing samples in a large batch, and the accuracy and the precision of determined results are high.

Description

technical field [0001] The invention relates to a method for detecting tobacco active substances, in particular to a method for measuring total solanesol in tobacco and tobacco products. Background technique [0002] Tobacco is the plant with the highest content of solanesol. Solanesol has antibacterial, anti-inflammatory, cardiovascular disease and anti-ulcer effects. It is an irreplaceable intermediate in the synthesis of coenzyme Q10 and vitamin K2, and can be used to synthesize the anticancer synergist SDB . [0003] At present, the detection of tobacco solanesol generally adopts chromatographic analysis method. Due to the large molecular weight and high boiling point of solanesol, the determination of solanesol by gas chromatography requires complex derivatization treatment. Liquid chromatography is a commonly used method for solanesol. [0004] Solanesol exists in tobacco in two forms: free alcohol and compounded state (mainly acetate, palmitate, linoleate). Therefore...

AI Technical Summary

Problems solved by technology

Existing tobacco solanesol is generally determined by Soxhlet extraction with non-polar solvents (n-hexane, petroleum ether, etc.), the extract is saponified under heating and reflux in a water bath, and the saponified extract is obtained by liquid-liquid extraction Total solanesol determination solution, the method is cumbersome, the steps are complicated, the measurement period is long, the detection efficiency is low, it is not conducive to batch operation, and consumes a large amount of organic solvents

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