Separation and preparation method for four glycoside chemical reference substances in Tibetan capillary artemisia

A technology for storing Yinchen and reference substances, which is applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., to achieve the effects of good stability and repeatability, shortening separation time, and reducing consumption of organic solvents
CN103408602AInactive Publication Date: 2013-11-27CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
Publication Date
2013-11-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a separation and preparation method for four glycoside chemical reference substances in Tibetan capillary artemisia. The method comprises steps of (1) subjecting a Tibetan capillary artemisia medicinal material to alcohol extraction and concentration to obtain a Tibetan capillary artemisia extract; (2) extracting with chloroform and n-butanol separately, after the Tibetan capillary artemisia extract is dissolved, to obtain a chloroform part, an n-butanol part and a water part; (3) drying the n-butanol part to obtain dry powder of the n-butanol part; (4) dissolving the dry powder of the n-butanol part and injecting into a preparative high-performance liquid chromatography, and obtaining a component group of the four compounds after elution, on-line ultraviolet detection and drying; (5) pumping the stationary phase and the mobile phase of a solvent system into a high-speed countercurrent chromatography, and separating the component group, after the high-speed countercurrent chromatography is balanced, to obtain a four-glycoside mixture with a purity larger than 90%; and (6) dissolving the mixture, adding into a gel separation column, eluting and drying to obtain the four glycoside chemical reference substances with purities larger than 98%. The method is short in separation time, low in organic solvent consumption, and good in stability and repeatability.
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Description

technical field

[0001] The invention relates to a preparation method of a chemical reference substance, in particular to a method for separating and preparing four kinds of glycoside chemical reference substances from Capum chinensis. Background technique

[0002] Tibetan Yinchen is used in Tibetan medicine to treat various diseases of the liver and gallbladder such as hepatitis, cholecystitis and gallbladder and jaundice. At present, it is impossible to stably obtain a chemical reference substance with a purity of more than 98% from Capillaris, which cannot meet the quality control requirements for the creation of new drugs and related preparations, which restricts the medicinal development and research of capillary.

[0003] Scholars at home and abroad have also separated and purified the chemical components of gentiopicroside, mangiferin, swertigin, and isoorientin. For example, the patent CN102503996A has separated and purified gentiopicroside from Cape chinensis, but no...

Claims

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