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Preparation method of tuna skin collagen micro-molecular peptides

A technology of small molecule peptides and tuna, which is applied in the field of extraction and preparation of fish skin collagen, can solve the problems of a large amount of fresh water and energy, which do not conform to the mainstream, and achieve the goal of improving extraction rate and purity, reducing production costs, and reducing production energy consumption Effect

Inactive Publication Date: 2013-12-04
NINGBO BOFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hot water extraction method needs to use a large amount of fresh water and energy, which is not in line with the mainstream of national and world economic development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0008] Example 1: After soaking and cleaning the raw tuna skin, first use a pulverizer to pulverize it into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 30 microns, and mix with 6 times the volume of water evenly Then enter the high-speed centrifuge at 12,000 rpm for 30 minutes to remove non-collagenous water-soluble substances, and the collagen-containing precipitate and 3 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The compound enzyme is mainly composed of trypsin, papain and collagenase, the added amount is 0.5% of the raw material, the reaction conditions are temperature 37°C, pH 8.0, and reaction time 2 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 3KD. Those with a molecular weight less than 3KD pass through the ultrafiltration...

example 2

[0009] Example 2: After soaking and cleaning raw tuna skin, first use a pulverizer to pulverize it into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 50 microns, and mix with 2 times the volume of water evenly Then enter the high-speed centrifuge at 8000 rpm for continuous centrifugation for 30 minutes to remove non-collagen water-soluble substances, and the collagen-containing precipitate and 5 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The compound enzyme mainly consists of trypsin, papain and collagenase, the added amount is 2% of the raw material, the reaction conditions are temperature 50°C, pH 7.5, and reaction time 4 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 3KD. Those with a molecular weight less than 3KD pass through the...

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Abstract

The invention discloses a preparation method of tuna skin collagen micro-molecular peptides. The preparation method comprises the following steps: immersing and cleaning tuna skins, crushing the tuna skins by using a crusher, adding to an industrial grade colloid mill, carrying out ultramicro crushing to a particle size of 30-50mum, uniformly mixing with water having a volume 2-6 times the volume of obtained particles, adding to a centrifuge, continuously centrifuging at 8000-12000rpm for 30min, removing the obtained supernatant, adding the obtained precipitate and water having a volume 3-5 times the volume of the obtained precipitate to a biological enzymatic hydrolysis pot, carrying out enzymatic hydrolysis under conditions comprising a temperature of 37-50DEG C and a pH value of 7.5-8.0 for 2-4h, processing the obtained enzymatic hydrolysis product by a 3KD ultrafilter membrane, carrying out nanofiltration membrane concentration of the part having a molecular weight below 3KD in the product, and carrying out spray drying to obtain the finally-finished collagen micro-molecular peptides polypeptides, wherein the addition amount of a composite enzyme in the above enzymatic hydrolysis reaction is 0.5-2% of the amount of a raw material. The part having a molecular weight above 3KD in the enzymatic hydrolysis product returns to the biological enzymatic hydrolysis pot for enzymatic hydrolysis.

Description

technical field [0001] The invention belongs to a method for extracting seafood, in particular to a method for extracting and preparing fish skin collagen. technical background [0002] At present, tuna skin is used as the leftovers of the tuna processing process, as a waste or to produce low-value feed, and its economic value is low. As a high-quality marine economic fish, tuna has a huge market demand and a large amount of tuna skin produced. Rich in high-quality active collagen. Collagen, as an important biological tissue structural protein, has attracted more and more attention and rise, with huge market potential and high economic value, especially collagen small molecule active peptides, which are widely used in medicine, beauty and health care and food industry. The traditional process of collagen extraction can be divided into five categories, namely acid method, alkali method, enzymatic method, salt method and hot water extraction method. The basic principle is t...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K2/00C07K1/34
Inventor 张建芳
Owner NINGBO BOFENG BIOTECH