Microbiological oil sludge treatment process

A microbial treatment and biological treatment technology, applied in the direction of biological sludge treatment, sludge treatment, etc., can solve the problems of oil sludge pollution, high operating costs, secondary pollution, etc., and achieve the effect of low cost and low power consumption

Active Publication Date: 2013-12-18
JIANGSU BODA ENVIRONMENTAL PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the above methods have a large one-time investment, troublesome operation and management, and high operating costs. The most important thing is that the treatment is not thorough, which is easy to cause sec

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The sewage sludge near the hotel in Yixing City was selected to be screened by the following methods:

[0021] 1.1 Strain enrichment

[0022] Add a certain amount of oil sludge (2%) to the glucose medium in the Erlenmeyer flask, put it in a shaker, and cultivate it at 30° C. for 3 days to obtain a strain enrichment solution. The formula of glucose medium is: sodium chloride 1g, calcium chloride 0.5g, dipotassium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.5g, ferric chloride 0.02g, ammonium nitrate 1g, magnesium sulfate heptahydrate, 1g yeast extract , 2g glucose, 1L water, pH 7.0-7.2; sterilize at 110°C for 20min.

[0023] 1.2 strain screening

[0024] After diluting the enrichment solution, streak and separate on the glucose solid medium plate (2% agar is added to the glucose medium), culture at 30°C for 24 hours, pick a single colony that grows well on the plate and streak Inoculate on an alkane plate, culture at 30°C for 48 hours, pick out the two l...

Embodiment 2

[0026] Biological and biochemical tests were carried out on the Y-57 strain. The yeast YPD agar was cultured on the plate (30°C) for 3 days, and the colony could grow to 1-2 mm, and the color was white to cream. The initial colony was smooth, and after prolonged culture, the colony appeared wrinkled. When growing on solid or liquid medium, the cell shape is round or oval, and sometimes the cells are elongated and grow like false filaments, and the cell size is about 4-6×6-8 microns.

[0027] The yeast was identified as Candida lipolytica with the Biolog Microorganism Automatic Analyzer produced by Biolog Company of the United States.

[0028] Biological and biochemical tests were carried out on the P-101 strain. The bacterium was cultured aerobically on a nutrient agar plate for 24 hours. The bacteria grew vigorously, and the colonies were smooth, moist, translucent and light yellow colonies, and produced a strong smell. The Gram staining was negative bacillus , extremely man...

Embodiment 3

[0032] Candida lipolytica Y-57 (CGMCC NO.5790) and Pseudomonas putida P-10 (CGMCC NO.5801) were cultured in LB medium at 30°C, 200rpm shaker for 24 hours, and then according to 5 % of the inoculum was inoculated into the fermentation medium, stirred at 30°C, 200rpm, air volume 1:0.4-0.5, and tank pressure 0.05mpa for 36 hours to obtain Candida lipolytica Y-57 and CGMCC No.5790. Pseudomonas putida P-101 mixed culture solution with preservation number CGMCC No.5801.

[0033] Fermentation medium (g / L): glucose 10, liquid paraffin 15, yeast extract 3.0, magnesium sulfate 0.2, potassium chloride 0.2, potassium dihydrogen phosphate 2.0, urea 1.0, ammonium nitrate 1.5, Tween-80 0.5ml, trace Saline solution 1.0ml. pH natural.

[0034] Among them: every liter of trace salt solution contains 11.0 grams of zinc sulfate, 6.0 grams of manganese sulfate, 1.0 grams of ferrous sulfate, EDTA-Na 2 7.3 grams, 0.3 grams of cobalt sulfate, 40 mg of copper sulfate, 60 mg of boric acid, 1 mg of p...

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PUM

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Abstract

The invention belongs to the environment-friendly field and discloses a microbiological oil sludge treatment process. The microbiological oil sludge treatment process comprises four steps of oil sludge fluidization, biological treatment, sludge dehydration and biological induction, wherein the biological treatment step is to add candida lipolytica Y-57 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5790 and pseudomonas putida P-101 with the preservation number of CGMCC No.5801 into a biological reaction tank, wherein after aeration biological reaction, in terms of absolute dry mud, the oil content of the oil sludge is less than 2%, and the water contains less than 150mg/L of oil; floating oil on the upper part of water surface is recycled by an oil receiving device, fine sediment on the bottom is exhausted after reaching the standards, and the oil sludge enters the sludge dehydration step. According to the microbiological oil sludge treatment process disclosed by the invention, a microbiological treatment method, a biological induction method and a mechanical method are combined for application, so that the oil content of the sludge reaches 0.3%-standard to recover a sludge environment, thereby achieving a purpose of recycling and hazard-free treatment.

Description

technical field [0001] The invention belongs to the field of environmental protection and relates to a process for treating oil sludge by microorganisms. Background technique [0002] Oily sludge is oily solid waste produced during oil extraction, transportation, refining and oily sewage treatment. It contains a large amount of hydrocarbons, benzene series, phenols, anthracene and other substances. If it is not treated, it will not only pollute the environment, but also be a waste of resources. [0003] Each station in Huabei Oilfield has the problem of difficult disposal of oil sludge. Now it is necessary to treat the settled oil sludge in the settling tanks of each plant station so that it can be discharged safely and harmlessly. [0004] At present, there are many ways to deal with oil sludge, such as: landfill method, mechanical method, incineration method, composting method, land cultivation method, bioreactor method and plant method. Generally speaking, the above met...

Claims

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Application Information

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IPC IPC(8): C02F11/02
CPCY02W10/20
Inventor 刘关平王鹏杜郭君
Owner JIANGSU BODA ENVIRONMENTAL PROTECTION
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