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Urease inhibitor composition

A technology of urease inhibitor and composition, applied in the field of urease inhibitor composition, urease inhibitor composition with gossypol as active substance

Active Publication Date: 2013-12-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gossypol is low-toxic and has no residue. However, except for a small amount of gossypol used as a contraceptive, it is still in the research stage and initial stage as a raw material for other drugs. There is no report on the use of gossypol as a urease inhibitor in fertilizers.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of Urease Inhibitors

[0037] Instrument: WGZ-2XJ bacterial turbidimeter (instrument) (Shanghai Xinrui Instrument Co., Ltd.), HH-2 digital display constant temperature water bath (Changzhou Aohua Instrument Co., Ltd.), DHG 9070A electric heating constant temperature blast drying oven ( Shanghai Jinghong Experimental Equipment Co., Ltd.)

[0038] Reagents: gossypol acetate (≥98.0%, Shaanxi Ciyuan Biotechnology Co., Ltd.), PVP (K15), PVP (K25), PVP (K30) (Aladdin Reagent (Shanghai) Co., Ltd.), dichloromethane (Sinopharm Group Chemical Reagent Co., Ltd.), absolute ethanol (Nanjing Chemical Reagent Co., Ltd.), urea (Guangdong Guanghua Chemical Factory Co., Ltd.), urease (activity>4U / mg, Shanghai Jinsui Biotechnology Co., Ltd.). All reagents used in the experiment were of analytical grade.

[0039] The following are some embodiments of the present invention:

[0040] Inhibitor 1 The preparation process:

[0041] S1. Accurately weigh the solids ...

Embodiment 2

[0077] Example 2 Performance testing of inhibitors

[0078] 1. Dispersion experiment of urease inhibitor composition in water, detection of sedimentation performance

[0079] Take 0.01g of samples (inhibitors 1-9 prepared in Example 1) in a beaker, add 5mL of distilled water, ultrasonically disperse until the solution is clear, and then transfer all the samples to the sample bottle. After the bacterial turbidity meter is preheated for 30 minutes, perform zero adjustment and calibration, put it into the sample bottle filled with liquid, read the turbidity value (in MCF) and the corresponding voltage value (in mV) at regular intervals, and draw the graph . The sedimentation curve of gossypol acetate dispersion is shown in the appendix figure 1 shown. attached by figure 1 It can be seen that within 0 to 7 days, the analysis of the turbidity and its corresponding voltage data shows that PVP basically has no effect on the change of the turbidity of the dispersion. In the PVP...

Embodiment 3

[0080] Example 3 Urease inhibitory performance test of urease inhibitor composition

[0081] 1. Preparation of urease solution Accurately weigh 0.0190 g of urease, dissolve it in distilled water in a beaker, transfer the solution to a 25 mL volumetric flask, dilute to volume, and filter. Pipette 20 mL of the filtrate into a 100 mL volumetric flask, and distilled water to volume.

[0082] 2. Preparation of reaction solution In each 50 mL volumetric flask, add different concentrations of inhibitor solution and 3 mL urease solution, and place the volumetric flask in a water bath at 37 °C for pre-incubation. After 1 h, add a little pH7.0 phosphate buffer solution and 1 mL of 10% urea to the volumetric flask, and adjust the phosphate buffer solution to volume, and place it in a water bath at 37 °C for 0.5 h.

[0083] 3. Detection method With no urea added as a reference, it is determined by indophenol blue spectrophotometry. Take 2 mL of the solution from each volumetric flask ...

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Abstract

The invention relates to the technical field of fertilizers, and specifically relates to a urease inhibitor composition. The composition is prepared from gossypol acetate and polyvinylpyrrolidone (PVP). According to the invention, a significant synergistic effect between PVP and gossypol acetate is found, and an appropriate compatibility proportion is found, so that good synergistic and solubilizing effects are achieved, and compared with the gossypol acetate, the obtained composition is convenient to use, powder is easier to store, the application effect is better, and the technical defects that existing urease inhibitors are uneasily dispersed in water, inconvenient to use and high in toxicity, and have residues are overcome, therefore, the urease inhibitor composition has an important practical popularization and application value, and provides powerful technical support for applying gossypol acetate as a urease inhibitor for inhibiting soil urease to coated slow release fertilizers or used with fertilizers.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a urease inhibitor composition, more specifically, to a urease inhibitor composition with gossypol as an active substance. Background technique [0002] Urea has many excellent properties. It is currently the most widely used nitrogen fertilizer in agricultural production in the world and in my country. It accounts for about 50% of the world's nitrogen fertilizer production and consumption market. It is also used by ruminants such as cattle, sheep, and rabbits to synthesize their own proteins ( MCP) is the cheapest and widely used non-protein nitrogen (NPN) feed, and is also a metabolite of a large number of nitrogen-containing compounds in humans and animals. As a chemical fertilizer, it can promote ear size and grain weight, nourish roots and leaves, strengthen stalks and prevent lodging. Urea itself is non-toxic and can exist stably in aqueous solution, but urease (Urease)...

Claims

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Application Information

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IPC IPC(8): C05G3/08C05G3/90
Inventor 卢其明吴佩琪陈敏廖俊健
Owner SOUTH CHINA AGRI UNIV
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